姓名：别小妹
性别：女
毕业院校：南京农业大学最高学位：博士
办公地址：食品科技学院 421 室办公电话：025-84396570
电子邮箱：bxm43@njau.edu.cn
研究方向：食品微生物与生物技术，食源性致病菌安全控制个人简介：
教授，博士生导师。研究方向为食品微生物与生物技术。2009.1-2010.1
为美国加利福尼亚大学戴维斯分校（UCDavis）微生物与分子遗传系访问学者。南京农业大学“钟山学者计划”学术骨干。曾经担任“食品加工与安全”专业学位授权点点长，食品质量与安全系系主任。国家重点研发计划
“食品安全关键技术研发”重点专项和国家自然科学基金面上项目等评审专家。主持国家自然科学基金面上项目、国家“863”计划项目、 "十三五"国家重点研发计划-子课题和江苏省科技项目等，发表 SCI
论文 100 余篇（通讯作者及合作作者）。参加获得教育部科技进步二等奖 1 项和江苏省高等教育教学成果二等奖 1
项。参加《食品微生物》（南农）和《食品安全》（浙大）国家级精品课建设。副主编教材《现代食品生物技术》和《果蔬贮藏加工及质量管理技术》两部。
本科教学：主讲“食品生物技术”、“食品微生物”、“微生物遗传育种学”和
“食品安全生物技术检测”等课程。
研究生教学：负责“现代食品生物技术”（中英文）、“食品质量安全检测新技

术”, 参加“食品科学技术进展”和“高级食品微生物学”等课程。
科研情况：
1、研究方向介绍
1）食源性致病菌安全控制：食源性致病菌快速分子检测、生物被膜形成机制、环境胁迫机制、耐药性和毒理等相关机制及安全控制研究。
2）微生物抗菌肽：芽孢杆菌和乳酸菌抗菌肽结构表征、抑菌机制和 NRPS 分子修饰、抗菌肽分子设计、食品防腐、乳酸菌改善肠道功能研究。
2、主持科研项目
1）国家自然科学基金面上项目：Bacillomycin D 复合酶系重组介导的新型抗菌脂肽合成规律探索（2019-2023）。
2）国家自然科学基金面上项目：基于枯草芽孢杆菌 fengycin 合成酶结构修饰的新型抗菌脂肽探索（2012-2016）。
3）国家自然科学基金面上项目：枯草芽孢杆菌菌株 fmbJ 新型抗菌物质结构鉴定及对桃软腐病作用机理（2006-2009）。
4）"十二五"国家科技支撑项目：食源性致病微生物快速检测试剂盒和仪器的研发与产业化-食品中主要致病菌微孔板生物芯片快速检测技术研究（2012-2015）。
5) "十三五"国家重点研发计划“: 食品中生物性及放射性危害物高效识别与确证关

键技术及产品研发”项目-子课题(2018-2021)。
6）国家“863”项目：功能性结构酯合成脂肪酶基因的定向进化及其高效表达
（2008-2011）。
7）江苏省农业自主创新项目:即食果蔬中食源性致病菌快速检测关键技术及装备开发（2018-2021）。

8）江苏省社会发展项目实时荧光 RNA 恒温扩增单增李斯特菌检测技术研究
（2012-2015）。
9）江苏省自然基金项目：功能性结构酯高效生产脂肪酶的分子定向进化研究
（2007-2010）。
10）江苏省科技项目：芽孢杆菌来源的高效广谱食品防腐剂的研究（2003-2006）。
科研成果：
[1] Wan JJ, GuoJP, Lu.ZX,Bie X M*, Lv F X, Zhao H Z. Development of a test kit
for visual loop-mediated isothermal amplification of Salmonella in spiked ready-to-eat
fruits and vegetables[J]. Journal of Microbiological Methods. 2020,169:105583.
[2] Wan JJ, Lu.ZX, Bie X M*, Lv F X, Zhao H Z. Improvement of a new selective enrichment broth for
culturing Salmonella in ready-to-eat fruits and vegetables[J]. Journal of Food Safety. 2020,
e12817:1-12.https://doi.org/10.1111/jfs.12817.
[3] Qiao JJ, Zhu MJ, Lu.ZX, Lv F X, Zhao H Z., Bie X M*.The antibiotics resistance mechanism and
pathogenicity of cold stressed Staphylococcus aureus. LWT-FOOD SCIENCE AND TECHNOLOGY.
2020,126:109274.
[4] Fan Y, Qiao JJ, Lu ZX, Feng ZY, Tao Y, Lv FX, Zhao HZ, Zhang C, Xiaomei Bie*
.Study of the Mechanism of Biofilm Formation in Listeria monocytogenes. Food Research
International. 2020,137:109405.
[5] Hai D; Yin XP, Lu ZX, Lv FX, Zhao HZ, Bie X M*.
Occurrence,drugresistance, and virulence genes of Salmonella isolated from chicken
and eggs. FOOD CONTROL. 2020, 113: 107109. https://doi.org/10.1016/j.foodcont.2020.107109.
[6] Ma Z, Zhang R J, Hai D, Lu Z X, Lv F X, Zhao H Z, Zhang C, McAllisterb T A, Stanfordc K, Bie X
M*. Antibio?lm activity and modes of action of a novel β-sheet
peptide against multidrug-resistant Salmonella enterica[J]. Food Research International. 2019,
125(9): 1-9.
[7] Ma Z, Bumunang E W, Stanford K, Bie X M*, Niu Y D, McAllister T A. Biofifilm formation
by Shiga toxin-producing Escherichia coli on stainless steel coupons as affected by temperature
and incubation time[J]. Microorganisms, 2019, 95: 7.
[8] Ma Z, Kim S, Bie X M*, Niu Y, McAllister T A. Effects of beef juice on biofilm formation by
Shiga

toxin-producing Escherichia coli on stainles steel[J]. Foodborne pathogens and disease, 2019, 12.
[9] Zhai L G, Liu H X, Chen Q M, Lu Z X, Chong Zhang, Lv F X, Bie X M*. Development of a real-time
nucleic acid sequence–based amplification assay for the rapid detection of Salmonella
spp. from food[J]. Brazilian Journal of Microbiology, 2019, 50: 1.
[10] Zhai L G, Li J J, Tao T T, Lu Z X, Lv F X, Bie X M*. Propidium monoazide-
real-time PCR amplification for viable Salmonella species and Salmonella Heidelberg in
pork[J]. Canadian Ournal Of Microbiology, 2019, 65: 7.
[11] Gao L, Guo J P, Fan Y, Ma Z, Lu Z X, Zhang C, Zhao H Z, Bie X M*. Mdule
and individual domain deletions of NRPS to produce plipastatin derivatives in Bacillus
subtilis[J]. Microbial Cell Factories, 2018, 17(1): 84.
[12] Ju X Y, Li J J, Zhu M J, Lu Z X, Lv F X, Zhu X Y, Bie X M* Effect of the
luxS gene on biofilm formation and antibiotic resistance by Salmonella serovar
Dublin[J]. Food Research International. 2018, 107: 385-393.
[13] Han J Z, Ma Z, Gao P, Lu Z X, Liu H X, Gao L, Lu W J, Ju X Y, Lv F X, Zhao H Z, Bie X M*. The
antibacterial activity of LI-F type peptide against methicillin-resistant Staphylococcus
aureus (MRSA) in vitro and inhibition of infections in murine scalded
epidermis[J]. Applied Microbiology and Biotechnology, 2018, 2018(102): 2301– 2311.
[14] Ju X Y, Zhu M J, Han J Z, Lu Z X, Zhao H Z, Bie X M*. Combined effects and cross-interactions
of different antibiotics and polypeptides in Salmonella bredeney[J]. Microbial Drug Resistance.
2018, 24(10): 1450-1459.
[15] Ma Z, Han J Z, Chang B X, Gao L, Lu Z X, Lv F X, Zhao H Z, Zhang C, Bie
X M*. Membrane-active amphipathic peptide WRL3 with in vitro antibiofilm capability and
in vivo efficacy in treating methicillin-resistant Staphylococcus aureus burn wound infections[J].
ACS Infectious Diseases, 2017, 3(11): 820-832.
[16] Han J Z, Gao P, Zhao S M, Bie X M*, Lu Z X, Zhang C, Lv F X. iTRAQ-based proteomic analysis of
LI-F type peptides produced by Paenibacillus polymyxa JSa-9 mode of action against
Bacillus cereus[J]. Journal of Proteomics, 2017, 150: 130-140.
[17] Liu J M, Zhai L G, Lu W J, Lu Z X, Bie X M*. Amino acid
decarboxylase-dependent acid tolerance, selected phenotypic, and virulence gene expression
responses of Salmonella

enterica serovar Heidelberg[J]. Food Research International, 2017, 92: 33-39.

[18] Han J Z, Wang F, Gao P, Ma Z, Zhao S M, Lu Z X, Lv F X, Bie X M*. Mechanism of action of
AMP-jsa9, a LI-F-type antimicrobial peptide produced by Paenibacillus polymyxa
JSa-9, against Fusarium moniliforme[J]. Fungal Genetics and Biology, 2017, 104: 44-45.
[19] Han J, Zhao S, Ma Z, Gao L, Liu H, Muhammad U, Lu Z X, Lv F X, Bie X M*. The antibacterial
activity and modes of LI-F type antimicrobial peptides against Bacillus cereus in
vitro[J]. Journal of Applied Microbiology, 2017,123(3): 602-614.
[20] Gao L, Han J Z, Liu H X, Qu X X, Lu Z X, Bie X M*. Plipastatin and surfactin coproduction by
Bacillus subtilis pB2-L and their effects on microorganisms[J]. International Journal of General
and Molecular Microbiology, 2017:110(8): 1007-1018.
[21] Gao L, Liu H X, Ma Z, Han J Z, Lu Z X, Dai C, Lv F X,Bie X M*. Translocation of the
thioesterase domain for the redesign of plipastatin synthetase[J]. Scientific reports, 2016, 6:
38467.
[22] Ma Z, Yang J, Han J Z, Gao L, Liu H X, Lu Z X, Zhao H Z, Bie X M*.
Insights into the antimicrobial activity and cytotoxicity of engineered α-helical peptide
amphiphiles[J]. Journal of Medicinal Chemstry. 2016, 59(24): 10946-10962.
[23] Zhao S M, Han J Z, Bie X M*, Lu Z X, Zhang C, Lv F X. Purification and
characterization of plantaricin JLA-9: a novel bacteriocin against Bacillus spp.
produced by Lactobacillus plantarum JLA-9 from Suan-Tsai, a traditional Chinese
fermented cabbage[J]. Journal of Agricultural and Food Chemistry, 2016, 64 (13): 2754–2764.
[24] Liu H X, Gao L, Han J Z, Ma Z, Lu Z X, Dai C, Zhang C, Bie X M*. Biocombinatorial synthesis of
novel lipopeptides by COM domain-mediated reprogramming of the plipastatin NRPS
complex[J]. Frontiers in Microbiology, 2016, 7: 1081.
[25] Liu H X, Qu X X, Gao L, Zhao S M, Lu Z X, Zhang C, Bie X M*. Characterization of a Bacillus
subtilis surfactin synthetase knockout and antimicrobial activity analysis[J].
Journal of Biotechnology, 2016, 237: 1-12.
[26] Jiang J, Bie X M*, Gao L, Lu Z X, Liu H X, Zhang C, Lv F X, Zhao H Z. Identification of
novel surfactin derivatives from NRPS modification of Bacillus subtilis and its
antifungal activity against Fusarium moniliforme[J]. BMC Microbiol, 2016, 16(1): 31.
[27] Yu Q, Zhai L G, Bie X M, Lu Z X, Zhang C, Tao T T, Li J J, Lv F X, Zhao
H Z. Survey of five food-borne pathogens in commercial cold food dishes and their detection by
multiplex PCR[J].

Food Control, 2016,59(1): 862–869.

[28] Li J J, Zhai L G, Bie X M*, Lu Z X, Kong X H, Yu Q, Lv F X, Zhang C, Zhao H Z. A novel visual
loop-mediated isothermal amplification assay targeting gene62181533 for the detection of
Salmonella spp. in foods[J]. Food Control, 2016, 60(2): 230–236.
[29] Mao S, Gao P, Lu Z X, Lv F X, Zhang C, Zhao H Z, Bie X M*. Engineering of a
thermostable β-1,3-1,4-glucanase from Bacillus altitudinis YC-9 to improve its catalytic
efficiency[J]. Journal of the Science of Food and Agriculture, 2016, 96(1): 109–115.
[30] Ying Q, Ansong E, Diamond A M, Lu Z X, Yang W C, Bie X M*. Quantitative proteomic analysis
reveals that anti-cancer effects of selenium-binding protein in vivo are associated with
metabolic pathways[J]. Plos One, 2015, 10(5): e0126285.
[31] Yao S L, Zhao S M, Lu Z X, Gao Y Q, Lv F X, Bie X M*. Control of agitation and aeration rates
for the production of surfactin in foam overflowing fed-batch culture with
industrial fermentation[J]. Revista Argentina de Microbiologia, 2015, 47(4): 344-349.
[32] Zhai L G, Kong X H, Lu Z X, Lv F X, Zhang C, Bie X M*. Detetion of Salmonella enterica serovar
Dublin by polymerase chain reaction in multiplex format[J]. Journal of
Microbiological Methods, 2014, 100: 52-57.
[33] Deng Y, Ju Y J, Lu Z X, Lv F X, Yan D, Bie X M*. Identification of
4″-isovaleryl-spiramycin III produced by Bacillus sp. fmbJ[J]. Archives of Microbiology, 2014, 196
(2): 87-95.
[34] Zhai L G, Yu Q, Bie X M*, Lu Z X, Lv F X, ZhangC, Kong X H, Zhao H Z. Development of a PCR
test system for specific detection of Salmonella Paratyphi B in foods[J]. FEMS
Microbiology Letters. 2014, 355: 83-89.
[35] Tang Q Y, Bie X M*, Lu Z X, Lv F X, Tao Y, Qu X X. Effects of fengycin from Bacillus subtilis
fmbJ on apoptosis and necrosis in Rhizopus stolonife[J]. Journal of Microbiology, 2014, 52:
675-680.
[36] Kong X H, Lu Z X, Zhai L G, Yao S L, Zhang C, Lv F X, Bie X M*. Mining and evaluation of new
specific molecular targets for the PCR detection of Salmonella spp. Genome[J]. World Journal of
Microbiology and Biotechnology, 2013, 29: 2219–2226.
[37] Mao S R, Lu Z X, Zhang C, Lv F X, Bie X M*. Purification, characterization, and heterologous
expression of a thermostable β-1,3-1,4-Glucanase from Bacillus altitudinis YC-9[J].
Applied Biochemistry and Biotechnology, 2013,169: 960–975.
[38] Gaidenko T A,Bie X M, Baldwin E P, Price C W*. Two Surfaces of a conserved
interdomain

linker differentially affect output from the RST sensing module of the Bacillus
subtilis

stressosome[J]. Journal of Bacteriology, 2012, 194(15): 3913-3921.

[39] Deng Y, Lu Z X, Lv F X, Zhang C, Wang Y, Zhao H Z, Bie X M*. Identification of LI-F
antibiotics and di-n-butyl phthalate produced by Paenibacillus polymyxa[J]. Journal of
Microbiological Methods, 2011, 85: 175?182.
[40] Zhou X H, Lu Z X, Lv F X, Zhao H Z, Wang Y, Bie X M*. Antagonistic action of Bacillus subtilis
strain fmbj on the postharvest pathogen Rhizopus stolonifer[J]. Journal of Food Science, 2011, 76:
254?259.
[41] Deng Y, Lu Z X, Bi H, Lv F X, Zhang C, Bie X M*. Isolation and
characterization of peptide antibiotics LI-F04 and polymyxin B6 produced by Paenibacillus
polymyxa strain JSa-9[J]. Peptides, 2011, 32: 1917?1923.
[42] Gaidenko T A, Bie X M, Baldwin E P, Price C W*. Substitutions in the presumed
sensing domain of the Bacillus subtilis stressosome affect its basal output but not
response to environmental signals[J]. Journal of Bacteriology, 2011, 193: 3588–3597.
[43] Bie X M, Lu Z X, Lv F X. Identification of fengycin homologues from Bacillus
subtilis with ESI-MS/CID[J]. Journal of Microbiological Methods, 2009, 79: 272–278.
[44] Lu Y J, Dong X, Liu S, Bie X M*. Characterization and identification of a
novel marine Streptomyces sp. produced antibacterial substance[J]. Marine
Biotechnology, 2009, 11: 717–724.
[45] Bie X M, Lu Z X, Lv F X. Preservative effect of the antimicrobial substance
from Bacillus subtilis fmbJ on pasteurized milk during storage[J]. Food Science and Technology
International, 2006, 12(3): 189-194.
[46] Bie X M, Lu Z X, Lv F X, Zeng X X. Screening the main factors affecting
extraction of the antimicrobial substance from Bacillus subtilis fmbJ using the
Plackett–Burman method[J]. World Journal of Microbiology and Biotechnology, 2005, 21: 925–928.