姓 名：刘 斐
性 别：女
毕业院校：中国科学技术大学（本科）/美国凯斯西储大学（博士）
最高学位：博 士
办公地址：逸夫楼 3036/教十一楼 A110办公电话：025-84395856
电子邮箱：feiliu24@njau.edu.cn
研究方向：（1）动物免疫及疫病防控技术
（2）食品安全与动物健康领域的快速现场检测
（3）微生物营养与代谢
（4）单分子检测技术研发
（5）智能成像与传感
个人主页：www.sinmolab.com

个人简介：
刘斐教授、博导。国家优秀青年基金获得者，全国青年联合会委员，教育部 “动物健康与食品安全”国际合作联合实验室副主任，中国畜牧兽医学会动物生理生化学分会理事。2004
年本科毕业于中国科学技术大学，随后赴美国凯斯西储大学攻读博士学位，毕业后于加州大学伯克利分校做博士后。2012
年被南京农业大学作为高层次人才引进。刘斐博士回国后先后入选国家优秀青年基金、江苏省“六大人才高峰计划（A 类）”、江苏省双创博士以及江苏省“333
高层次人才培养工程”第三层次培养对象（中青年学术技术带头人）。首届盛彤笙兽医科学奖青年教师奖获得者以及江苏省第四届创新创业大赛三等奖获得者。主持国家自然基金面上项目、国家自然基金优秀青年项目、国家科技
支撑计划子课题、国家重点研发计划子课题、江苏省社会发展面上项目、江苏省自然基金青年项目等多项课题。刘斐教授长期从事动物免疫及疫病防控技术、食品安全与动物健康领域的快速现场检测、微生物营养与代谢、单分子
检测技术研发、智能成像与传感等方面的基础前沿科学和工程技术研究工作。研究成果在 PNAS ， Biosens Bioelectron，ACS Appl. Mater.
Interfaces，Cell，Sensor Actuat B: Chem，Analytica Chimica Acta 等刊物发表 SCI 论文 33 篇，累计影响因子
187.97，累计引用次数超过 500 次。

科研项目：
1. 江苏省农业自主创新资金（SCX(19)3410），“一体化单病毒荧光检测系统的研发及在 POCT 中的应用”，2019/06-2021/05，30 万，主持，在研；
2. 国家自然基金面上项目（3187011172），“基于单颗粒示踪技术的猪流行性腹泻病毒侵染宿主细胞的分子机制研究”，2019/1-2022/12，59 万，主持，在研；

3. 国家重点研发计划项目子课题（2018YFD0500102），“畜禽重要病原共感染与协同致病机制”, 2018/06-2020/12，41 万，主持，在研；
4. 江苏省重点研发计划社会发展面上项目（BE2018709），“便携式单病毒荧光检测系统的研发及其在高致病性禽流感 H7N9 检测中的应用研究”，2018/7- 2021/6，50
万，主持，在研；
5. 国家自然基金优秀青年项目（31522056），“基础兽医学”，2016/1-2018/12，
130 万，主持，已结题；
6. 国家科技支撑计划项目子课题（2015BAD12B01），“病毒入侵的单分子分析”， 2015/1-2019/12，70 万，主持，在研；
7. 国家自然科学基金面上项目（31372399），“DExH/D-box RNA 解螺旋 RHA 重塑 RNA 结构与调控口蹄疫病毒 RNA
复制的分子机制”，2014/1-2017/12，82万，主持，已结题；
8. 江苏省自然科学基金青年项目（BK20130669），“DExH/D-box RNA 解螺旋 RHA 重塑RNA 结构与调控口蹄疫病毒RNA
复制的分子机制”，2013/7-2016/6， 20 万，主持，已结题。

荣誉奖项：
1. 江苏省“六大人才高峰”计划 A 类资助，“单分子技术的研发及其在单病毒快速检测中的运用”（2017 年）
2. 张家港创新创业领军人才计划“针对畜牧场动物病毒的便携式单分子检测系统”（2017 年）
3. 首届盛彤笙兽医科学奖青年教师奖（2016 年）
4. 国家优秀青年基金获得者（2016 年）
5. 江苏省“333 高层次人才培养工程”第三层次培养对象（中青年学术技术带头人）（2016 年）
6. 江苏省第四届创新创业大赛三等奖“针对畜牧场动物病毒的便携式单分子检测系统”（2016 年）
7. 动物医学院“绩效金奖”（2015 年）
8. 勃林格殷格翰奖教金（2014 年）
9. 江苏省双创博士（2013 年）

发明专利：
已授权发明专利：
? 一种特异性纳米金 DNA 探针的制备方法 ZL201310495123.2
? 一种用于单分子病毒检测玻片组 ZL201721617727.X
已申请发明专利：
? 一种利用全内反射荧光显微镜检测单个病原分子的方法
201710122250.6

? 一种利用血糖仪定量检测生物标识物的方法 201610804250.X
? 一种检测乙肝病毒的试剂盒及乙肝病毒的检测方法 201810166722.2

近年代表性论著：
1. Dan Jian#, Bin Wang#, Huachuan Huang, Xin Meng, Cheng Liu, Liang Xue, Fei Liu*, Shouyu
Wang*, “Sunlight based handheld smartphone spectrometer” Biosensors and Bioelectronics (2019),
143,111632. (IF=9.518)
2. Xufei Feng, Wenjie Kang, Xuping Wu, Shouyu Wang, and Fei Liu*, “Quantitative Detection and
Real-Time Monitoring on Endogenous mRNA in Single Live Cell Level Using Ratiometric Molecular
Beacon” ACS Appl. Mater. Interfaces (2019), 11, 32, 28752-28761. (IF=8.456)
3. Wenjie Kang#, Huachuan Huang#, Ming Cai#, Yangyang Li, Wei Hou, Feng Yun, Xuping Wu, Liang
Xue, Shouyu Wang*, Fei Liu*, “On-Site Cell Concentration and Viability Detections using Smartphone
based Field-Portable Cell Counter”, Analytica Chimica Acta (2019), 1077, 216-224. (IF=5.256)
4. Yanke Shan#, Bin Wang#, Huachuan Huang#, Dan Jian, Xuping Wu, Liang Xue, Shouyu Wang*, Fei
Liu*, “On-site quantitative Hg2+measurements based on selective and sensitive fluorescence
biosensor and miniaturized smartphone fluorescence microscope” Biosensors and Bioelectronics
(2019), 162: 238-247. (IF=9.518)
5. Wei Hou#, Yangyang Li#, Wenjie Kang, Xin Wang, Xuping Wu, Shouyu Wang, Fei Liu*, “Real-time
analysis of quantum dot labeled single porcine epidemic diarrhea virus moving along the
microtubules using single particle tracking” Scientific Reports (2019), 9(1): 1307. (IF=4.011)
6. Qi Wei#, Yangyang Li#, Javier Vargas, Jian Wang, Qingtao Gong, Yan Kong, Zhilong
Jiang, Liang Xue, Cheng Liu, Fei Liu*, and Shouyu Wang*, “Principal component analysis-based
quantitative differential interference contrast microscopy” Optics Letters (2019), 44:
45-48. (IF=3.866)
7. Jiahao Li, Xin Wang, Yanke Shan, Huachuan Huang, Dan Jian , Liang Xue , Shouyu
Wang , Fei Liu*, “Handheld Inkjet Printing Paper Chip Based Smart Tetracycline Detector”
Micromachines (2019), 10(1), 27. (IF=2.426)
8. Yanke Shan, Yan Zhang, Wenjie Kang, Bin Wang, Jiahao Li, Xuping Wu, Shouyu Wang, Fei Liu*,
“Quantitative and Selective DNA Detection with Portable Personal Glucose Meter using
Loop-based DNA Competitive Hybridization Strategy” Sensors and Actuators B: Chemical (2019) 282:
197-203. (IF=6.393)
9. Yanke Shan, Qingtao Gong, Jian Wang, Jing Xu, Qi Wei, Cheng Liu, Liang Xue, Shouyu Wang, and
Fei Liu*, “Measurements on ATP induced cellular fluctuations using real-time dual view
transport of intensity phase microscopy” Biomedical Optics Express, 2019; 10(5): 2337–2354.
(IF=3.91)

10. Qingyun Xie, Jing Liu, Yanke Shan, Shouyu Wang, Fei Liu*, “Substrate Determinants
for Unwinding Activity of the DExH/D-Box Protein RNA Helicase A” Biochemistry (2018), 57:
6662-6668. (IF=2.952)
11. Junbao Hu, Xin Meng, Qi Wei, Yan Kong, Zhilong Jiang, Liang Xue, Fei Liu, Cheng Liu, Shouyu
Wang, “Numerical tilting compensation in microscopy based on wavefront sensing using transport of
intensity equation method” Journal of Optics (2018), online. (IF=2.753)
12. Aihui Sun, Yan Kong, Zhilong Jiang, Wei Yu, Fei Liu, Liang Xue, Shouyu Wang, Cheng Liu,
“Sub-aperture switching based ptychographic iterative engine （sasPIE） method for quantitative
imaging” Optics Communications (2018), 410: 514-519. (IF=1.961)
13. Ting Liu, Wenqi Wang Fei Liu Shouyu Wang, “Photochemical deposition fabricated highly
sensitive localized surface plasmon resonance based optical fiber sensor” Optics Communications
(2018), 427: 301-305. (IF=1.961)
14. Junbao Hu, Yan Kong, Zhilong Jiang, Liang Xue, Fei Liu, Cheng Liu, Shouyu Wang, “Adaptive
dual-exposure fusion-based transport of intensity phase microscopy” Applied Optics
(2018), 57: 7249-7258. (IF=1.973)
15. Zhilong Jiang, Yan Kong, Fei Liu Cheng Liu, Shouyu Wang, “Graphics processing unit (GPU) aided
wavefront-based autofocusing in microscopy” AIP Advances (2018), 8(10). (IF=1.579)
16. Jing Cao, Yan Zhang, Yanke Shan, Jingui Wang, Fei Liu*, Hongrui Liu, Gang Xing, Jing
Lei, and Jiyong Zhou,“A pH-dependent Antibacterial Peptide Release Nano-system Blocks Tumor Growth
in vivo without Toxicity” Scientific Reports (2017), 7(1): 11242. (IF=4.011)
17. Jing Xu, Xiaolin Tian, Xin Meng, Yan Kong, Shumei Gao, Haoyang Cui, Fei Liu, Liang Xue, Cheng
Liu, Shouyu Wang, “Wavefront-sensing-based autofocusing in microscopy” Journal of Biomedical Optics
(2017), 22: 1-7. (IF=2.555)
18. Yunbin Xu, Fei Liu, Juan Liu, Dandan Wang, Yan Yan, Senlin Ji, Jie Zan, Jiyong Zhou, “The
co-chaperone Cdc37 regulates the rabies virus phosphoprotein stability by targeting to Hsp90AA1
machinery” Scientific Reports (2016), 6: 27123. (IF=4.011)
19. Jianyu Fang, Haiyan Wang, Juan Bai, Qiaoya Zhang, Yufeng Li, Fei Liu, and Ping Jiang, “Monkey
Viperin Restricts Porcine Reproductive and Respiratory Syndrome Virus Replication” PloS One
(2016),11(5): e0156513. (IF=2.776)
20. Fulin Zhao, Qingyun Xie, Mingfei Xu, Shouyu Wang, Jiyong Zhou, Fei Liu*, “RNA
aptamer based electrochemical biosensor for sensitive and selective detection of
cAMP” Biosensors and Bioelectronics (2015), 66: 238-243. (IF=9.518)
21. Qingyun Xie, Fulin Zhao, Hongrui Liu, Yanke Shan, Fei Liu*, “A label-free and

self-assembled electrochemical biosensor for highly sensitive detection of cyclic diguanylate
monophosphate (c-di-GMP) based on RNA riboswitch” Analytica Chimica Acta (2015), 882:22-26.
(IF=5.256)
22. Liang Xue, Javier Vargas, Shouyu Wang, Zhenhua Li, Fei Liu*, “Quantitative
interferometric microscopy cytometer based on regularized optical flow algorithm” Optics
Communications (2015), 350: 222–229. (IF=1.961)
23. Andrea A. Putnam, Zhaofeng Gao (共同一作), Fei Liu, Huijue Jia, Quansheng Yang & Eckhard
Jankowsky*, “Division of labor in an oligomer of the DEAD-box RNA helicase Ded1p” Molecular Cell
(2015), 59: 1-12. (IF=14.548)
24. Wei Zhang, Shangxin Song, Fei Liu, Yi Liu, Yuanshu Zhang, “Beta-casomorphin- 7 prevents
epithelial-mesenchymal transdifferentiation of NRK-52E cells at high glucose level: Involvement of
AngII-TGF-β1 pathway” Peptides (2015), 70: 37- 44. (IF=2.659)
25. Shouyu Wang, Keding Yan, Yanke Shan, Mingfei Xu, Fei Liu, Liang Xue, “Phase measurements of
erythrocytes affected by metal ions with quantitative interferometric microscopy”
Optical Engineering (2015), 54(12): 124105. (IF=1.209)
26. Jing Cao, Chao Feng, Yan Liu, Shouyu Wang, and Fei Liu*, “Highly sensitive and rapid
bacteria detection using molecular beacon–Au nanoparticles hybrid nanoprobes,”
Biosensors and Bioelectronics (2014), 57: 133–138. (IF=9.518)
27. Liang Xue, Shouyu Wang, Keding Yan, Nan Sun, Zhenhua Li, and Fei Liu*, “Fast pixel shifting
phase unwrapping algorithm in quantitative interferometric microscopy” Chinese Optics
Letters (2014), 12, 071801. (IF=1.907)
28. Liang Xue, Shouyu Wang, Keding Yan, Nan Sun, Pietro Ferraro, Zhenhua Li, and Fei Liu*,
“Gravity driven high throughput phase detecting cytometer based on quantitative
interferometric microscopy” Optics Communications (2014), 316: 5–9. (IF=1.961)
29. Liang Xue, Shouyu Wang, Keding Yan, Nan Sun, Zhenhua Li, and Fei Liu*,
“Quantitative interferometric microscopy with improved full-field phase
aberration compensation” Optical Engineering (2014), 53 (11), 113105. (IF=1.209)
30. Hee-Kyung Kim, Fei Liu, Jingyi Fei, Carlos Bustamante, Ruben L. Gonzalez Jr, and Ignacio
Tinoco Jr.*, “A frameshifting stimulatory stem loop destabilizes the hybrid state and impedes
EF-G-catalyzed translocation,” Proc. Natl. Acad. Sci. USA (2014), 111: 5538–5543. (IF=9.58)
31. Fei Liu*, Andrea Putnam, and Eckhard Jankowsky*, “DEAD-Box Helicases Form
Nucleotide-Dependent, Long-Lived Complexes with RNA” Biochemistry (2014), 53: 423–433. (IF=2.952)
32. Huijue Jia, Xuying Wang, Fei Liu, Ulf-Peter Guenther, Sukanya Srinivasan, James

T. Anderson, and Eckhard Jankowsky*, “The RNA Helicase Mtr4p Modulates Polyadenylation in
the TRAMP Complex” Cell (2011), 145: 890-901. (IF=36.216)
33. Fei Liu*, Andrea Putnama, and Eckhard Jankowsky*, “ATP hydrolysis is required for DEAD-box
protein recycling but not for duplex unwinding,” Proc. Natl. Acad. Sci. USA (2008), 105:
20209-20214. (IF=9.58)
Highlighted in: Editor’s Choice, Science 323, 310 (2009).