王鸣华
南京农业大学，植物保护学院，教授，博士生导师
电话：
e-mail：wangmha@njau.edu.cn
地址：江苏省南京市，卫岗1号，南京农业大学植物保护学院，210095
教育经历：
1999/9 - 2004/6，南京农业大学，农药学，博士，导师：蒋木庚
1983/9 - 1986/6，中国农业大学，农药学，硕士，导师：陈馥衡
1979/9 - 1983/6，兰州大学，有机化学，学士
工作经历：
2006/12 – 今，南京农业大学，植物保护学院，教授
2003/3 – 2006/12，南京农业大学，植物保护学院，副教授
1989/12 - 2003/3，南京农业大学，理学院，副教授
1986/7 - 1989/12，南京农业大学，理学院，讲师
研究方向：
1、农药残留免疫分析
2、手性农药环境行为与毒理
3、农药残留降解
主持或参加科研项目及人才计划项目情况：
1、国家重点研发计划，2016YFD**、经作体系农药沉降行为及减施因素、2016/01-2020/12、775万元、主持。
2、国家自然科学基金面上项目，**、烟碱类杀虫剂噬菌体展示多肽竞争物作用机制与定量构效关系研究、2015/01-2018/12、80万元、主持。
3、公益性行业（农业）科研专项经费项目，、农作物主要病虫害新药剂、新剂型研究与应用、2012/01-2016/12、60万元、参加。
4、“十二五”农村领域国家科技计划课题，2012BAD14B02、循环农业系统污染物减控关键技术研究、 2012/01-2016/12、20万元、参加。
5、国家863项目，2011AA100806、食品加工过程安全控制工程化技术研究及设备开发、2011/01-2015/12、66万元、主持。
6、国家公益性行业科研专项，**、外来入侵害虫西花蓟马防控技术研究与示范、2008/01-2010/12、150万元、主持。
7、国家863项目，2006AA10Z447、化学农药多残留免疫检测、2006/01-2008/12、15万、参加。
8、江苏省自然科学基金项目，BK**、拟除虫菊酯多残留免疫分析及免疫识别机制的研究、2005/06-2008/7、7.5万、主持。
9、农业部农药残留标准转化项目多项。
10、农业部农药登记残留试验项目多项。
论文（2012至今）
1. Hongjie You, Xiude Hua*, Lu Feng, Nana Sun, Qi Rui, Limin Wang, Minghua Wang*, Competitive immunoassay for imidaclothiz using upconversion nanoparticles and gold nanoparticles as labels. Microchim Acta 2017, 184: 1085–1092.
2. Xiude Hua, Yuan Ding, Jiachuan Yang, Ming Ma, Haiyan Shi, Minghua Wang*, Direct competitive ?uoroimmunoassays for detection of imidaclothiz in environmental and agricultural samples using quantum dots and europium as labels. Science of the Total Environment 2017, 583: 222–227.
3. Haiyan Shi, Enze Sheng, Ming Ma, Xiude Hua, Minghua Wang*, Development of an enhanced colloidal gold immunochromatographic assay for detection of imidaclothiz. Chinese Journal of Analytical Chemistry, 2017, 45: 403-408.
4. Mingming Tian , Qing Zhang, Xiude Hua, Bowen Tang, Beibei Gao, Minghua Wang*, Systemic stereoselectivity study of flufiprole: Stereoselective bioactivity, acute toxicity and environmental fate. Journal of Hazardous Materials 2016, 320: 487–494.
5. Enze Sheng, Haiyan Shi, Liangliang Zhou, Xiude Hua, Lu Feng, Tong Yu, Minghua Wang*, Dual-labeled time-resolved fluoroimmunoassay for simultaneous detection of clothianidin and diniconazole in agricultural samples. Food Chemistry 2016 192: 525–530.
6. Qing Zhang, Haiyan Shi, Beibei Gao, Mingming Tian, Xiude Hua, Minghua Wang*, Enantioseparation and determination of chiral phenylpyrazole insecticide ethiprole in agricultural and environmental samples and its enantioselective degradation in soils. Science of the Total Environment 2016, 542: 845–853.
7. Qing Zhang, Mingming Tian, Beibei Gao, Haiyan Shi, Xiude Hua, Minghua Wang*, Enantioseparation and determination of the triticonazole enantiomers in fruits, vegetables, and soil using efficient extraction and clean-up methods. J Chromatography B 2016, 1009-1010: 130–137.
8. Gao Beibei, Zhang Qing, Tian Mingming, Zhang Zhaoxian, Wang Minghua*, Enantioselective determination of the chiral pesticide isofenphos-methyl in vegetables, fruits, and soil and its enantioselective degradation in pak choi using HPLC with UV detection. Analytical and Bioanalytical Chemistry 2016, 408: 6719–6727.
9. Ming Ma, Mo Chen, Lu Feng, Hongjie You, Rui Yang, Anna Boroduleva, Xiude Hua, Sergei A. Eremin, Minghua Wang*, Fluorescence Polarization Immunoassay for Highly Efficient Detection of Imidaclothiz in Agricultural Samples. Food Anal. Methods 2016, 9:2471–2478.
10. Xiude Hua, Liangliang Zhou, Lu Feng, Yuan Ding, Haiyan Shi, Limin Wang, Shirley J. Gee, Bruce D. Hammock, Minghua Wang*, Competitive and noncompetitive phage immunoassays for the determination of benzothiostrobin. Analytica Chimica Acta 2015, 890: 150–156.
11. Baiping Zhao, Xiude Hua, Fei Wang, Weiliang Dong, Zhoukun Li, Yu Yang, Zhongli Cui, Minghua Wang*, Biodegradation of propyzamide by Comamonas testosterone W1 and cloning of the propyzamide hydrolase gene camH. Bioresource Technology 2015, 179: 144–149.
12. Qing Zhang, Xiude Hua, Yu Yang, Wei Yin, Mingming Tian, Haiyan Shi, Minghua Wang*, Stereoselective degradation of flutriafol and tebuconazole in grape. Environmental Science and Pollution Research 2015, 22: 4350 – 4358.
13. Wei Yin, Xiude Hua, Xiaofeng Liu, Haiyan Shi, Shirley J. Gee, Minghua Wang*, Bruce D. Hammock, Development of an enzyme-linked immunosorbent assay for thiacloprid in soil and agro-products with phage-displayed peptide. Analytical Biochemistry 2015, 481: 27–32.
14. Xiude Hua, Xiaofeng Liu, Wei Yin, Yazhong Xia, Qiujun Zhou, Yiwen Lu, Wei Li, Haiyan Shi, Fengquan Liu, Minghua Wang*, A sensitive monoclonal antibody-based enzyme-linked immunosorbent assay for the detection of bifenthrin in a chemical soil barrier. Science of the Total Environment 2015, 502: 246–251.
15. Meiyun Wang, Xiude Hua, Qing Zhang, Yu Yang, Haiyan Shi, Minghua Wang*, Enantioselective Degradation of Metalaxyl in Grape, Tomato, and Rice Plants. Chirality 2015, 27: 109–114.
16. Ming Li, Ming Ma, Xiude Hua, Haiyan Shi, Qiuxia Wang and Minghua Wang*, Quantum dots-basedfluoroimmunoassay for the simultaneous detection of clothianidin and thiacloprid in environmental and agricultural samples. RSC Advance 2015, 5: 3039–3044.
17. Qing Zhang, Xiude Hua, Haiyan Shi, Jisong Liu, Mingming Tian, Minghua Wang*, Enantioselective bioactivity, acute toxicity and dissipation in vegetables of the chiral triazole fungicide flutriafol. Journal of Hazardous Materials 2015, 284: 65–72.
18. Mingming Tian, Qing Zhang, Haiyan Shi, Beibei Gao, Xiude Hua, Minghua Wang*, Simultaneous determination of chiral pesticide flufiprole enantiomers in vegetables, fruits, and soil by high-performance liquid chromatography. Analytical and Bioanalytical Chemistry 2015, 407: 3499–3507.
19. Qing Zhang,Liangliang Zhou, Yu Yang, Xiude Hua, Haiyan Shi, Minghua Wang*, Study on the stereoselective degradation of three triazole fungicides in sediment. Ecotoxicology and Environmental Safety 2015, 117: 1–6.
20. Lujing Cong, Jing Guo, Jisong Liu, Haiyan Shi, Minghua Wang*, Rapid degradation of endosulfan by zero-valent zinc in water and soil. Journal of Environmental Managent 2015, 150:451–455.
21. Xiude Hua, Wei Yin, Haiyan Shi, Ming Li, Yanru Wang, Hong Wang, Yonghao Ye, Hee Joo Kim, Shirley J. Gee, Minghua Wang, Fengquan Liu*, Bruce D. Hammock, Development of phage immuno-loop-mediated isothermal amplification assays for organophosphorus pesticides in agroproducts. Analytical Chemistry 2014, 86: 8441?8447.
22. Ming Li, Xiude Hua, Ming Ma, Jisong Liu, Liangliang Zhou, Minghua Wang*, Detecting clothianidin residues in environmental and agricultural samples using rapid, sensitive enzyme-linked immunosorbent assay and gold immunochromatographic assay. Science of the Total Environment 2014, 499: 1–6.
23. Xiude Hua, Xiaofeng Liu, Haiyan Shi, Yanru Wang, Hee Joo Kim, Shirley J. Gee, Minghua Wang*, Fengquan Liu, Bruce D. Hammock, Development of a heterologous enzyme-linked immunosorbent assay for organophosphorus pesticides with phage-borne peptide. RSC Advance 2014, 4: 42445–42453.
24. Xu Yan, Xiaojian Tang, Hongxia Li, Enze Sheng, Dongdong Yang, Minghua Wang*, Rapid Detection of Four Organophosphorous and neonicotinoid toxicants using bi-enzyme tracer-based competitive enzyme-linked immunosorbent assay. Food Analytical Methods, 2014, 7: 1186–1194.
25. Yulong Yuan, Xiude Hua, Ming Li, Wei Yin, Haiyan Shi, Minghua Wang*, Development of a sensitive indirect competitive enzyme-linked immunosorbent assay based on the monoclonal antibody for the detection of benzothiostrobin residue. RSC Advance 2014, 4: 24406–24411.
26. Dongdong Yang, Wei Yin, Lujing Cong, Minghua Wang*, Synthesis and characterization of a molecularly imprinted polymer for preconcentration of clothianidin in environmental samples. Analytcal Letter, 2014, 47(15): 2613–2627.
27. Meiyun Wang, Qing Zhang, Lujing Cong, Wei Yin, Minghua Wang*, Enantioselective degradation of metalaxyl in cucumber, cabbage, spinach and pakchoi. Chemosphere 2014, 95: 241–246.
28. Ming Li, Enze Sheng, Yulong Yuan, Xiaofeng liu, Xiude Hua, Minghua Wang*, Sensitive time-resolved fluoroimmunoassay for quantitative determination clothianidin in agricultural samples. Environmental Science and Pollution Research 2014, 21 (9): 5803–5809.
29. Qing Zhang, Mingming Tian, Meiyun Wang, Haiyan Shi, Minghua Wang*, Simultaneous enantioselective determination of triazole fungicide Flutriafol in vegetables, fruits, wheat, soil and water by reversed-phase high-performance liquid chromatography. Journal of Agricultural and Food Chemistry, 2014, 62: 2809?2815.
30. Chengying Lu, Xing Yin, Xiaofeng Liu, Minghua Wang*, Study of the Photodegradation Kinetics and Pathways of Hexaflumuron in Liquid Media. Photochemistry and Photobiology 2014, 90 (6): 1219–1223.
31. Ming Li, Xiaofeng Liu, Xiude Hua, Wei Yin, Qingkui Fang and Minghua Wang*, Fluorescence polarization immunoassay for highly efficient detection of clothianidin in agricultural samples. Analytical Methods 2014, 6: 6541–6547.
32. Haiyan Shi,Hongxia Li, Xiude Hua, Zuntao Zheng, Guonian Zhu, Minghua Wang*, Characterization of Multihapten Antigens on Antibody Sensitivity and Specificity for Parathion. Analytcal Letter 2014, 47 (16): 2699–2707.
33. Juhua Chang, Shaoying Liu, Shengli Zhou, Minghua Wang*, Guonian Zhu, Effects of butachlor on reproduction and hormone levels in adult zebrafish (Danio rerio). Experimental and Toxicologic Pathology 2013, 65 (1-2): 205–209.
34. Zhenjiang Liu, Ming Li, Haiyan Shi, Minghua Wang*, Development and Evaluation of an Enzyme-Linked Immunosorbent Assay for the Determination of Thiacloprid in Agricultural Samples. Food Analytical Methods 2013, 6 (2): 691–697.
35. Ming Li, Enze Sheng, Lujing Cong, Minghua Wang*, Development of Immunoassays for Detecting Clothianidin Residue in Agricultural Products. Journal of Agricultural and Food Chemistry 2013, 61: 3619–3623.
36. Zhenjiang Liu, Xu Yan, Xiuying Xu, Minghua Wang*, Development of chemiluminescence enzyme-linked immunosorbent assay for the simultaneous detection of imidaclothiz and thiacloprid in agricultural samples. Analyst 2013, 138 (11): 3280 – 3286.
37. Zhenjiang Liu, Xu Yan, Xiude Hua, Minghua Wang*, Time-resolved fluoroimmunoassay for quantitative determination of thiacloprid in agricultural samples. Analytical Methods 2013, 5: 3572–3576.
38. Kewei Ren, Yahui Jin, Mengmeng Cao, Minghua Wang*, Detection of Aryloxyphenoxypropionate Herbicides by Enzyme-linked Immunosorbent Assay. Analytcal Letter 2012, 45(8): 831–840.
39. Mengmeng Cao, Ming Li, Xu Yan, Minghua Wang*, Stereoselectivity of an enzyme-linked, immunosorbent assay for S-bioallethrin. Analytical Methods 2012, 4(2): 534–538.
40. Hu Zhang, Mingrong Qian, Xinquan Wang, Xiangyun Wang, Hao Xu, Qiang Wang, Minghua Wang*, HPLC–MS/MS enantioseparation of triazole fungicides using polysaccharide-based stationary phases. Journal of Separation Science 2012, 35(7): 773–781.
41. Juhua Chang, Wenjun Gui, Minghua Wang*, Guonian Zhu, Effects of butachlor on estrogen receptor, vitellogenin and P450 aromatase gene expression in the early life stage of zebra?sh. Journal of Environmental Science and Health, Part A 2012, 47(11): 1672–1677.
42. Zhenjiang Liu, Yahui Jin, Minghua Wang*, Determination of diniconazole in agricultural samples by sol-gel immunoaffinity extraction procedure coupled with HPLC and ELISA. PLos One 2012, 7(10): e46926.
43. Xu Yan, Haiyan Shi, Minghua Wang*, Development of an enzyme-linked immunosorbent assay for the simultaneous determination of parathion and imidacloprid. Analytical Methods 2012, 4 (12), 4053–4057.
44. Zhang Hu, Qian Mingrong, Wang Xinquan, Wang Xiangyun, Xu Hao, Qi Peipei, Wang Qiang, Wang Minghua*, Analysis of Tebuconazole and Tetraconazole Enantiomers by Chiral HPLC-MS/MS and Application to Measure Enantioselective Degradation in Strawberries. Food Analytical Methods 2012, 5(6): 1342–1348.
著作
1. 植物化学保护实验，北京大学出版社
2. 拟除虫菊酯化学，化学工业出版社
3. 农药营销管理学，中国农业出版社
4. 新杂环农药—杀虫剂，化学工业出版社
5. 新杂环农药—除草剂，化学工业出版社
6. 农药残留分析，中国农业出版社
7. 农药立体化学，化学工业出版社
专利
1. 王鸣华，赵百萍，华修德，一种可高效降解炔苯酰草胺的降解菌及其应用，ZL0.9。
2. 王鸣华；袁玉龙；华修德；施海燕，苯噻菌酯半抗原、人工抗原、特异抗体制备方法及其用途，ZL4.9。
3. 王鸣华；施海燕；李波，联苯菊酯抗原、抗体及其应用，ZL5.6。
4. 华修德，王鸣华，刘凤权，施海燕，噬菌体免疫环介导等温扩增检测法，ZL3.4。
5. 王鸣华，华修德，丁园， 氯噻啉抗体特异性结合的噬菌体展示多肽及其用途， 6.6。
6. 王鸣华，华修德，冯璐，施海燕，苯噻菌酯抗体特异性结合的多肽及其用途，2.8。
7. 华修德，王鸣华，周亮亮，丁园，苯噻菌酯免疫复合体特异性结合的多肽及其用途，4.7。
8. 王鸣华；华修德；殷维；施海燕，一种抗噻虫啉单克隆抗体及其用途，4.7。
9. 王鸣华；李明；施海燕，噻虫胺抗原、抗体及其应用，7.X。
10. 陆艺文；王鸣华；夏亚中；华修德；周秋君；李伟，抗联苯菊酯单克隆抗体及其用途，6X。
教学
1、农药学
2、农药加工与管理
3、农药研究新进展
4、植物保护研究方法
奖励
王鸣华（7/11），农产品与环境中农药、重金属快速检测技术与产品，中国植物保护学会，科学技术奖，一等奖，2016