| 第一作者： | Jiao, Kuibao |
|---|---|
| 英文第一作者： | Jiao, Kuibao |
| 联系作者： | Duan, Yadong；Li, Xingguo |
| 英文联系作者： | Duan, Yadong；Li, Xingguo |
| 发表年度： | 2023 |
| 卷： | 13 |
| 摘要： | As an excellent grafting material, Malus baccata (L.) Borkh is native to Liaoning, Jilin, Heilongjiang and other regions in China, with a strong adverse environmental adaptability. As a typical transcription factor, the NAC gene acts as a regulator in many molecular pathways responding to abiotic stress. However, research of NAC in the Malus baccata has just begun. In the present research, a new NAC transcription factor, MbNAC22, was obtained from the seedlings of Malus baccata, and its function in drought and salt treatments was studied by heterologous expression. The open reading frame of the MbNAC22 gene is 768, encoding 255 amino acids (aa). Through confocal microscopy, MbNAC22 was found to be located in the nucleus. The heterologous expression of MbNAC22 in Arabidopsis showed that it enhanced the viability of Arabidopsis under drought and salt treatments. Under stresses, the chlorophyll content of the plants decreased, but the decline of the overexpressed-MbNAC22 Arabidopsis was relatively low. Through phenotypic observation and determination of stress-related physiological indicators, it was found that compared with WT Arabidopsis, overexpressed-MbNAC22 Arabidopsis had a higher tolerance to stresses. Under stresses, the overexpression of MbNAC22 positively regulated ion-transport-related genes (AtNHX1 and AtSOS1), the key genes of the ABA pathway (AtNCED3 and AtDREB2A), the proline synthesis gene (AtP5CS2) and the drought-induced gene (AtERD11), while the expression of the leaf senescence-associated gene (AtSAG21) and programmed cell death related gene (AtAEP1) was inhibited. Therefore, we speculate that MbNAC22 responds positively to drought and salt stresses by regulating the expression of stress-related genes. |
| 刊物名称： | AGRONOMY-BASEL |
| 参与作者： | Jiao,KB(Jiao,Kuibao)[1];Han,JX(Han,Jiaxin)[2];Guo,BT(Guo,Baitao)[1];Wu,YQ(Wu,Yuqi)[1];Zhang,L(Zhang,Lei)[1];Li,YZ(Li,Yuze)[1];Song,Penghui[1];Han,Deguo[2];(Duan,Yadong[1],[3];Li,Xingguo[2] |
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MbNAC22, a Malus baccata NAC Transcription Factor, Increased Drought and Salt Tolerance in Arabidops
本站小编 Free考研考试/2024-01-13
MbNAC22, a Malus baccata NAC Transcription Factor, Increased Drought and Salt Tolerance in Arabidopsis
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