| 论文编号: | |
| 论文题目: | Multiplex CRISPR/Cas9-mediated knockout of soybean LNK2 advances flowering time |
| 英文论文题目: | Multiplex CRISPR/Cas9-mediated knockout of soybean LNK2 advances flowering time |
| 第一作者: | Li, Zhaobo |
| 英文第一作者: | Li, Zhaobo |
| 联系作者: | 孔凡江 |
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| 发表年度: | 2021 |
| 卷: | 9 |
| 期: | 4 |
| 页码: | 767-776 |
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| 英文摘要: | Flowering time is an important agronomic trait for soybean yield and adaptation. However, the genetic basis of soybean adaptation to diverse latitudes is still not clear. Four NIGHT LIGHT-INDUCIBLE AND CLOCK-REGULATED 2 (LNK2) homeologs of Arabidopsis thaliana LNK2 were identified in soybean. Three single-guide RNAs were designed for editing the four LNK2 genes. A transgene-free homozygous quadruple mutant of the LNK2 genes was developed using the CRISPR (clustered regularly interspaced short palindromic repeats)/Cas9 (CRISPR-associated protein 9). Under long-day (LD) conditions, the quadruple mutant flowered significantly earlier than the wild-type (WT). Quantitative real-time PCR (qRT-PCR) revealed that transcript levels of LNK2 were significantly lower in the quadruple mutant than in the WT under LD conditions. LNK2 promoted the expression of the legume-specific El gene and repressed the expression of FT2a. Genetic markers were developed to identify LNK2 mutants for soybean breeding. These results indicate that CRISPR/Cas9-mediated targeted mutagenesis of four LNK2 genes shortens flowering time in soybean. Our findings identify novel components in flowering-time control in soybean and may be beneficial for further soybean breeding in high-latitude environments. (C) 2021 Crop Science Society of China and Institute of Crop Science, CAAS. Production and hosting by Elsevier B.V. on behalf of KeAi Communications Co., Ltd. |
| 刊物名称: | Crop Journal |
| 英文刊物名称: | Crop Journal |
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| 英文参与作者: | Z. B. Li, Q. Cheng, Z. R. Gan, Z. H. Hou, Y. H. Zhang, Y. L. Li, H. Y. Li, H. Y. Nan, C. Yang, L. N. Chen, S. J. Lu, W. Q. Shi, L. Y. Chen, Y. P. Wang, C. Fang, L. |
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Multiplex CRISPR/Cas9-mediated knockout of soybean LNK2 advances flowering time
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