Publication in refereed journal
香港中文大学研究人员 ( 现职)
| 陈功教授 (外科学系) |
| 唐志辉教授 (耳鼻咽喉-头颈外科学系) |
| 尹怀信教授 (耳鼻咽喉-头颈外科学系) |
| 陈碧云教授 (病理解剖及细胞学系) |
| Professor VLANTIS Alexander Chris (耳鼻咽喉-头颈外科学系) |
| 王珊珊博士 (耳鼻咽喉-头颈外科学系) |
全文
| 数位物件识别号 (DOI) http://dx.doi.org/10.1002/jcb.25539 |
引用次数
Web of Sciencehttp://aims.cuhk.edu.hk/converis/portal/Publication/1WOS source URL
Scopushttp://aims.cuhk.edu.hk/converis/portal/Publication/1Scopus source URL
其它资讯
摘要Oxidative stress-induced DNA damage is a known causing factor for many types of tumors, but information on the role of oxidants and antioxidants in thyroid tumors is limited. The aim of this study was to determine antioxidant levels in thyroid tumors. In this study, tumor and its matched non-tumor thyroid tissue samples were obtained from 53 patients with thyroid tumors. The levels of manganese superoxide dismutase (MnSOD), thioredoxin reductase 2 (TXNRD2), glutathione (GSH), glutathione peroxidase (Gpx), catalase (CAT), and 27 kd heat-shock protein (hsp27) were determined in both thyroid tissue samples and cultured thyroid cells by immunohistochemical staining and western blot. Hydrogen peroxide (H2O2) was used to generate oxidant stress in the cell culture experiments. We found that the levels of MnSOD, TXNRD2, GSH, Gpx, and Hsp27 were increased in both malignant and benign tumors, while the level of CAT was decreased. To verify the results of the tissue study, we treated cultured thyroid cells with H2O2 and found the same pattern of antioxidant changes. Hsp27 was also increased after H2O2 treatment. The expression of hsp27 was upregulated by 8.24-, 6.96-, and 3.09-fold in thyroid cancer, follicular adenoma, multinodular goiter, respectively. Collectively, our study demonstrated that the levels of hsp27 together with MnSOD, TXNRD2, GSH, and Gpx were significantly upregulated by H2O2 in thyroid tumors. The increase of these antioxidants is observed in both malignant and benign tumors, particularly in the former. The upregulation of antioxidants is likely a protective mechanism of tumor cells to maintain their survival and growth. J. Cell. Biochem. http://aims.cuhk.edu.hk/converis/portal/Publication/1http://aims.cuhk.edu.hk/converis/portal/Publication/17: 2473–248http://aims.cuhk.edu.hk/converis/portal/Publication/1, 20http://aims.cuhk.edu.hk/converis/portal/Publication/16. ? 20http://aims.cuhk.edu.hk/converis/portal/Publication/16 Wiley Periodicals, Inc.
着者Wang S., Yang S., Vlantis A.C., Liu S.Y.W., Ng E.K.W., Chan A.B.W., Wu J., Du J., Wei W., Liu X., Liu Z., Ng S.K., van Hasselt C.A., Tong M.C.F., Chen G.G.
期刊名称Journal of Cellular Biochemistry
详细描述DOI http://aims.cuhk.edu.hk/converis/portal/Publication/10.http://aims.cuhk.edu.hk/converis/portal/Publication/1002/jcb.25539.
出版年份20http://aims.cuhk.edu.hk/converis/portal/Publication/16
月份http://aims.cuhk.edu.hk/converis/portal/Publication/1http://aims.cuhk.edu.hk/converis/portal/Publication/1
日期http://aims.cuhk.edu.hk/converis/portal/Publication/1
卷号http://aims.cuhk.edu.hk/converis/portal/Publication/1http://aims.cuhk.edu.hk/converis/portal/Publication/17
期次http://aims.cuhk.edu.hk/converis/portal/Publication/1http://aims.cuhk.edu.hk/converis/portal/Publication/1
出版社John Wiley & Sons Inc.
出版地United States
页次2473 - 248http://aims.cuhk.edu.hk/converis/portal/Publication/1
国际标準期刊号0730-23http://aims.cuhk.edu.hk/converis/portal/Publication/12
语言英式英语
关键词27 kd HEAT-SHOCK PROTEIN, ANTIOXIDANTS, OXIDANTS, THYROID TUMORS
