Publication in refereed journal
香港中文大学研究人员 ( 现职)
| 尹怀信教授 (耳鼻咽喉-头颈外科学系) |
| 陈基湘教授 (微生物学系) |
| Professor VLANTIS Alexander Chris (耳鼻咽喉-头颈外科学系) |
| 陈功教授 (外科学系) |
全文
| 数位物件识别号 (DOI) http://dx.doi.org/10.1016/j.canlet.2003.09.035 |
引用次数
Web of Sciencehttp://aims.cuhk.edu.hk/converis/portal/Publication/30WOS source URL
其它资讯
摘要Human papillomavirus type 16 (HPV 16) plays an etiological role in human laryngeal carcinoma. Apoptosis is closely associated with various biological processes including oncogenesis. This study investigated how HPV 16 oncoproteins E6 and E7 affect apoptosis in human laryngeal cancer cells. We established two human laryngeal cancer cell lines that expressed HPV 16 E6 and E7, respectively. Using these two cell lines, we found that both E6 and E7 exhibited an inhibitive effect on apoptosis induced by tumor necrosis factor alpha and cycloheximide. In both transfected cell lines, the expression of pro-apoptotic Bak was reduced and that of anti-apoptotic Bcl-2 was over-expressed. However, the expression of caspase-3 and caspase-8 was not significantly different between the E6- and E7-transfected cells and the control cells without HPV 16. p53 Protein was not detected in either the transfected or the non-transfected cells. Our study indicates that: (1) HPV 16 E6 and E7 oncoproteins are capable of inhibiting apoptosis in laryngeal squamous carcinoma cells; (2) the mechanism modulated by E6 and E7 involves the over-expression of Bcl-2 and the down-regulation of Bak; (3) the anti-apoptotic pathway is not related to the level of p53, caspase-3, or caspase-8. These results suggest that the dysregulation of apoptotic molecules Bak and Bcl-2 by HPV 16 E6 and E7 plays a role in the prolongation of cell survival, which may subsequently contribute to the development of human laryngeal cancer. (C) 2003 Elsevier Ireland Ltd. All rights reserved.
着者Du J, Chen GG, Vlantis AC, Chan PKS, Tsang RKY, van Hasselt CA
期刊名称Cancer Letters
出版年份2004
月份3
日期8
卷号205
期次1
出版社ELSEVIER SCI IRELAND LTD
页次81 - 88
国际标準期刊号0http://aims.cuhk.edu.hk/converis/portal/Publication/304-3835
电子国际标準期刊号1872-7980
语言英式英语
关键词Bak; Bcl-2; human papillomavirus type 16; laryngeal cancer; p53
Web of Science 学科类别Oncology; ONCOLOGY
