Publication in refereed journal
香港中文大学研究人员 ( 现职)
| 陈德章教授 (肿瘤学系) |
| 吴香玲教授 (病理解剖及细胞学系) |
| 郑淑?博士 (病理解剖及细胞学系) |
| 王如玲小姐 |
| 吕伟欣教授 (生物医学学院) |
| 洪波博士 (肿瘤学系) |
全文
| 数位物件识别号 (DOI) http://dx.doi.org/10.1016/j.bcp.2009.10.021 |
引用次数
Web of Sciencehttp://aims.cuhk.edu.hk/converis/portal/Publication/13WOS source URL
其它资讯
摘要YC-1 has recently been demonstrated to have potent anti-invasion and anti-metastatic activity in several cancer models, in addition to its anti-proliferation activity. However, the mechanism underlying its anti-invasion/anti-metastatic activity is largely unknown. Nasopharyngeal carcinoma (NPC) is a highly metastatic head and neck cancer in Southeast Asia. Here, we demonstrated that YC-1 inhibited invasiveness and proliferation of NPC cells, with the latter being accompanied by PARP cleavage, S-phase arrest and activation of Chk1/Chk2. We aimed at identifying novel anti-invasion mechanisms of YC-1 in NPC by a functional proteomic platform, the reverse phase protein array (RPPA). Our study revealed for the first time that multiple invasion-related signaling proteins (beta-catenin, caveolin, Src and EGFR), as well as several growth-related proteins (AMPK alpha, phospho-acetyl-CoA carboxylase (p-ACC), HER-2 and mTOR), which were previously un-described signaling proteins altered by YC-1, were found to be down-modulated by YC-1 in NPC cells. We hypothesized that YC-1-mediated downregulation of these invasion proteins contributed to its anti-invasion activity in NPC cells. Overexpression of EGFR, activated Src or caveolin, but not beta-catenin reversed the inhibitory effects of YC-1 on NPC cell invasion, with EGFR and activated Src having additional effects on rescuing NPC cells from YC-1-mediated growth inhibition. In summary, we have identified several novel anti-invasion mechanisms of YC-1 that could impact NPC, and possibly other cancers as well. (C) 2009 Published by Elsevier Inc.
着者Hong B, Lui VWY, Hui EP, Lu YL, Leung HSY, Wong EYL, Cheng SH, Ng MHL, Mills GB, Chan ATC
期刊名称Biochemical Pharmacology
出版年份2010
月份3
日期15
卷号79
期次6
出版社Elsevier
页次842 - 852
国际标準期刊号0006-2952
电子国际标準期刊号1873-2968
语言英式英语
关键词Anti-invasion; Anti-proliferation; Nasopharyngeal carcinoma (NPC); Reverse phase protein array; YC-1 mechanisms
Web of Science 学科类别Pharmacology & Pharmacy; PHARMACOLOGY & PHARMACY
