Publication in refereed journal
香港中文大学研究人员 ( 现职)
| 严秉泉教授 (外科学系) |
| 莫树锦教授 (肿瘤学系) |
| 李明月教授 (外科学系) |
| 陈功教授 (外科学系) |
全文
| 数位物件识别号 (DOI) http://dx.doi.org/10.1002/cncr.23166 |
引用次数
Web of Sciencehttp://aims.cuhk.edu.hk/converis/portal/Publication/17WOS source URL
其它资讯
摘要BACKGROUND. Cigarette smoking is well known to play an important role in the development of lung cancer. Inducible nitric oxide synthase (iNOS) can either promote or inhibit cell proliferation and growth, which makes its role in the development of malignant tumors controversial. The relation between cigarette smoking and iNOS in human lung cancer is unknown. METHODS. The study examined the levels of iNOS/NO in nonsmall-cell lung cancer (NSCLC) tissues of smokers and nonsmokers and in NSCLC cells (NCI-H23) treated by 4-(N-Methyl-N-nitrosamino)-1-(3-pyridyl)-1-butanone (NNK), a potent tobacco-specific carcinogen. RESULTS. The level of iNOS/NO was significantly higher in lung cancer tissues of smokers than that of nonsmokers. Unlike iNOS/NO, the activity of caspase-3 was reduced in the former compared with the latter. The expression of the cleaved caspase-3 was deceased in NCI-H23 cells treated with S-Nitroso-N-acetylpenicillamine (SNAP), an NO donor, whereas treatment with NG-methyl-L-arginine (NMA), an NO inhibitor, caused an increase in cleaved caspase-3. Consistent with the change in caspase-3, SNAP treatment inhibited cell death induced by UCNO1, a potent cell death-inducer. NMA treatment greatly enhanced the sensitivity of the cells to UCNO1. Further, the cells treated by NNK showed an increase in iNOS protein, accompanied by an elevation of cell proliferation. CONCLUSIONS. The study demonstrates that cigarette smoking promotes the level of iNOS/NO but suppresses the activity of caspase-3, which may lead to the proliferation and growth of lung cancer cells.
着者Chen GG, Lee TW, Xu H, Yip JHY, Li M, Mok TSK, Yim ARC
期刊名称Cancer
出版年份2008
月份1
日期15
卷号112
期次2
出版社JOHN WILEY & SONS INC
页次372 - 381
国际标準期刊号0008-543X
电子国际标準期刊号1097-0142
语言英式英语
关键词caspase-3; cell death; cigarette smoking; lung cancer; nitric oxide
Web of Science 学科类别Oncology; ONCOLOGY
