Publication in refereed journal
香港中文大学研究人员 ( 现职)
| 庄立信教授 (肿瘤学系) |
| 杜家辉教授 (病理解剖及细胞学系) |
| 许碧瑜博士 (病理解剖及细胞学系) |
| 邝约瑟教授 (妇产科学系) |
| 罗国炜教授 (病理解剖及细胞学系) |
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Web of Sciencehttp://aims.cuhk.edu.hk/converis/portal/Publication/233WOS source URL
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摘要We have investigated the genetic and epigenetic changes of a newly isolated tumor suppressor gene on 3p21.3, RASSF1A, in nasopharyngeal carcinoma (NPC), Four xenografts, four cell lines and 21 primary tumors were examined. Promoter hypermethylation of the 5 ' CpG island of RASSF1A was detected in 4 of 4 (100%) xenografts, in 3 of 4 (75%) cell lines, and in 14 of 21 (66.7%) primary tumors but not in the normal nasopharyngeal epithelia. Mutations were found in 2 of 21 (9.5%) primary tumors. In the cell lines and xenografts with extensive methylation, no RASSF1A gene expression was found, After treatment with 5 ' -aza-2 ' -deoxycytidine, reexpression and demethylation of the RASSF1A gene were detected in a NPC cell line. These findings suggest that promoter hypermethylation may participate in the transcriptional inactivation of the RASSF1A gene in NPC, The high incidence of RASSF1A alterations suggest that it is the critical target gene on chromosome 3p21.3 involved in the development of NPC.
着者Lo KW, Kwong J, Hui ABY, Chan SYY, To KF, Chan SC, Chow LSN, Teo PML, Johnson PJ, Huang DP
期刊名称Cancer Research
出版年份2001
月份5
日期15
卷号61
期次10
出版社AMER ASSOC CANCER RESEARCH
页次3877 - 3881
国际标準期刊号0008-5472
电子国际标準期刊号1538-7445
语言英式英语
Web of Science 学科类别Oncology; ONCOLOGY
