Publication in refereed journal
香港中文大学研究人员 ( 现职)
| 刘允怡教授 (外科学系) |
| 庄立信教授 (肿瘤学系) |
| 梁惠棠教授 (肿瘤学系) |
| 彭心鸣小姐 (肿瘤学系) |
| 赖宝山教授 (外科学系) |
| 王昭春教授 (病理解剖及细胞学系) |
全文
| 数位物件识别号 (DOI) http://dx.doi.org/10.1053/jhep.2000.19349 |
引用次数
Web of Sciencehttp://aims.cuhk.edu.hk/converis/portal/Publication/61WOS source URL
其它资讯
摘要The current paucity of cytogenetic information on hepatocellular carcinoma (HCC) reflects the difficulties in culturing hepatocytes in vitro. Here, we report on the successful culture of 15 HCC cases. Chromosome aneuploidy ranging from a near-diploid to hyperhexaploid karyotype was found, but their complete karyotypic interpretations were hampered by the presence of many unidentifiable rearrangements. Spectral karyotyping (SKY) was used to elucidate structural changes in these HCC samples and 3 liver cancer cell lines (PLC/PRF/5, Hep3B, and HepG2). Frequent structural abnormalities were found on chromosomes 1 (13 of 15 cases;3 of 3 cell lines), 8 (10 of 15 cases; 2 of 3 cell lines), 17 (9 of 15 cases; 3 of 3 cell lines), and 19 (9 of 15 cases; 1 of 3 cell lines). In particular, the chromosome regions 1p13-q21, 8p12-q21, 17p11-q12, 17q22, and 19p10-q13.1 were involved in multiple rearrangements. SKY analysis also suggested several previously undescribed breakpoints in HCC. These breakpoints, predominantly pericentromeric, clustered around the chromosome bands 2q33-q34, 3p13-q12, 4p14-q12, 5p10-q11, 7p12-q11, 10q10-q11, 11q10, 11q113-q21,12q10-q13,12q22-q23,13q10-q14,15q10, 18p11-q11, 20p11-q13.1, 21q10, and 22q10. When tumor sizes were compared, a significantly higher number of structural abnormalities was found in tumors larger than 4 cm (P = .007). Rearrangements such as t(1;8), t(1;11), t(1;19), and t(17;21) that were identified in both primary tumors and cell lines might represent markers that reflect proliferative advantages. Although SKY analysis did not indicate consistent translocations, it suggested nonrandom breakpoints, predominantly in the pericentromeric region, on a number of chromosomes. These breakpoint clusters may thus prove to be more important in the liver carcinogenesis and targets for further molecular investigations.
着者Wong N, Lai P, Pang E, Leung TWT, Lau JWY, Johnson PJ
期刊名称Hepatology
出版年份2000
月份11
日期1
卷号32
期次5
出版社W B SAUNDERS CO
页次1060 - 1068
国际标準期刊号0270-9139
电子国际标準期刊号1527-3350
语言英式英语
Web of Science 学科类别Gastroenterology & Hepatology; GASTROENTEROLOGY & HEPATOLOGY
