Publication in refereed journal
香港中文大学研究人员 ( 现职)
| 梁承晖教授 (肿瘤学系) |
| 庄立信教授 (肿瘤学系) |
| 莫树锦教授 (肿瘤学系) |
| 罗国炜教授 (病理解剖及细胞学系) |
| 杜家辉教授 (病理解剖及细胞学系) |
| 王昭春教授 (病理解剖及细胞学系) |
全文
| 数位物件识别号 (DOI) http://dx.doi.org/10.1016/S0046-8177(00)80216-6 |
引用次数
Web of Sciencehttp://aims.cuhk.edu.hk/converis/portal/Publication/49WOS source URL
其它资讯
摘要Nasopharyngeal carcinoma (NPC) is a commonly occurring tumor in southern China. Although several causative factors have now been recognized, the genetic basis underlying its tumorigenesis is still unclear. To identify potential chromosomal aberrations for further investigations, comparative genomic hybridization (CGH) analysis was applied to the study of genomic imbalances in 10 NPC biopsy specimens. Before CGH analysis, the tumor cell content within the biopsy specimens was enriched by tissue microdissection, and universal genome amplification was performed on the recovered DNA. Recurrent chromosomal gains were detected on Iq (6 of 10 cases), 2q (5 of 10 cases), 3q (7 of 10 cases), 6p (8 of 10 cases), 6q (5 of 10 cases), 7q11.2 (4 of 10 cases), 8q (6 of 10 cases), 11q13, 12, and 15q (8 of 10 cases each), 17q (6 of 10 cases), and 20q (5 of 10 cases). Common losses were identified on 3p (5 of 10 cases), 9p (5 of 10 cases), 11q14-qter (8 of 10 cases), and 14q (5 of 10 cases). Among these aberrations, 7, 8, and 11 gains were further investigated on a series of NPC tissue samples, by interphase fluorescent in situ hybridization (FISH), for the incidence of alpha-satellites: 7, 8, and 11 c-myc and Int-2. Low-level increases of alpha-satellite 7 (9 of 34 cases; 26.5%), alpha-satellite 8 (15 of 34 cases; 44%), and alpha-satellite 11 (8 of 32 cases; 25%) were detected, whereas high-level copy gains of c-myc (21 of 34 cases; 62%) and Int-2 (26 of 34 cases; 76.5%) were more frequently found. Our series is the first to identify genomic overrepresentations of c-myc and Int-2 in NPC. The high incidence of Int-2 amplifications strongly suggests a role of this proto-oncogene in the pathogenesis of NPC. Copyright (C) 2000 by W.B. Saunders Company.
着者Fan CS, Wong N, Leung SF, To KF, Lo KW, Lee SW, Mok TSK, Johnson PJ, Huang DP
期刊名称Human Pathology
出版年份2000
月份2
日期1
卷号31
期次2
出版社W B SAUNDERS CO
页次169 - 178
国际标準期刊号0046-8177
电子国际标準期刊号1532-8392
语言英式英语
关键词amplifications; c-myc; Int-2; nasopharyngeal carcinoma
Web of Science 学科类别Pathology; PATHOLOGY
