Publication in refereed journal
香港中文大学研究人员 ( 现职)
| 庄立信教授 (肿瘤学系) |
| 莫树锦教授 (肿瘤学系) |
| 梁承晖教授 (肿瘤学系) |
| 严秉泉教授 (外科学系) |
全文
| 数位物件识别号 (DOI) http://dx.doi.org/10.1002/(SICI)1097-0215(20000301)85:5<599::AID-IJC1>3.0.CO;2-# |
引用次数
Web of Sciencehttp://aims.cuhk.edu.hk/converis/portal/Publication/30WOS source URL
其它资讯
摘要The promyelocytic leukemia (PML) gene, which encodes a transformation and growth suppressor, was first identified at the chromosomal translocation break point t(15;17) in acute promyelocytic leukemia (PML). To determine if the PML gene might be involved in other neoplasias such as lung cancer, PML expression was analyzed by immunohistochemical staining and in situ hybridization. Considerable PML protein expression in the PML-oncogenic domain (POD) structure was found in adenocarcinomas (ADC) and squamous cell carcinomas (SCC) of the lung, but was almost completely absent in all the small cell lung carcinomas (SCLC) examined. In situ hybridization showed that both mRNA and DNA of PML were present in SCLC and in normal lung, suggesting that the decreased protein expression was due to either a defect in translation or protein instability, rather than the consequence of decreased transcription or gene deletion. Double staining showed that PML expression was inversely correlated with the proliferation marker Ki-67 and positively correlated with levels of apoptotic cells in these tumors. To determine if the precursor cells of SCLC, the neuroendocrine-producing cells, express PML, double labeling was performed with PML and chromogranin A, a bio-marker for neuroendocrine cells. Neuroendocrine cells from normal tissues were found to be PML positive, indicating that the lack of PML protein in SCLC is associated with the tumorigenic phenotype and is not the result of cell-lineage specificity. Thus, the decreased PML expression may play an important role in SCLC development. (C) 2000 Wiley-Liss, Inc.
着者Zhang P, Chin W, Chow LTC, Chan ASK, Yim APC, Leung SF, Mok TSK, Chang KS, Johnson PJ, Chan JYH
期刊名称INTERNATIONAL JOURNAL OF CANCER
出版年份2000
月份3
日期1
卷号85
期次5
出版社WILEY-LISS
页次599 - 605
国际标準期刊号0020-7136
电子国际标準期刊号1097-0215
语言英式英语
Web of Science 学科类别Oncology; ONCOLOGY
