Publication in refereed journal
香港中文大学研究人员 ( 现职)
| 陈维智博士 (药剂学院) |
| 凌美麟教授 (微生物学系) |
全文
| 数位物件识别号 (DOI) http://dx.doi.org/10.1128/AAC.47.11.3567-3573.2003 |
引用次数
Web of Sciencehttp://aims.cuhk.edu.hk/converis/portal/Publication/93WOS source URL
其它资讯
摘要A total of 88 salmonella isolates (72 clinical isolates for which the ciprofloxacin MIC was >0.06 mug/ml, 15 isolates for which the ciprofloxacin MIC was less than or equal to0.06 mug/ml, and Salmonella enterica serotype Typhimurium ATCC 13311) were studied for the presence of genetic alterations in four quinolone resistance genes, gyrA, gyrB, parC, and parE, by multiplex PCR amplimer conformation analysis. The genetic alterations were confirmed by direct nucleotide sequencing. A considerable number of strains had a mutation in parC, the first to be reported in salmonellae. Seven of the isolates sensitive to 0.06 mug of ciprofloxacin per ml had a novel mutation at codon 57 of parC (Tyr57-->Ser) which was also found in 29 isolates for which ciprofloxacin MICs were >0.06 mug/ml. Thirty-two isolates had a single gyrA mutation (Ser83-->Phe, Ser83-->Tyr, Asp87-->Asn, Asp87-->Tyr, or Asp87-->Gly), 34 had both a gyrA mutation and a parC mutation (29 isolates with a parC mutation of Tyr57-->Ser and 5 isolates with a parC mutation of Ser80-->Arg). Six isolates which were isolated recently (from 1998 to 2001) were resistant to 4 mug of ciprofloxacin per ml. Two of these isolates had double gyrA mutations (Ser83-->Phe and Asp87-->Asn) and a parC mutation (Ser80-->Arg) (MICs, 8 to 32 mug/ml), and four of these isolates had double gyrA mutations (Ser83-->Phe and Asp87-->Gly), one parC mutation (Ser80-->Arg), and one parE mutation (Ser458-->Pro) (MICs, 16 to 64 mug/ml). All six of these isolates and those with a Ser80-->Arg parC mutation were S. enterica serotype Typhimurium. One S. enterica serotype Typhi isolate harbored a single gyrA mutation (Ser83-->Phe), and an S. enterica serotype Paratyphi A isolate harbored a gyrA mutation (Ser83-->Tyr) and a parC mutation (Tyr57-->Ser); both of these isolates had decreased susceptibilities to the fluoroquinolones. The MICs of ciprofloxacin, levofloxacin, and sparfloxacin were in general the lowest of those of the six fluoroquinolones tested. Isolates with a single gyrA mutation were less resistant to fluoroquinolones than those with an additional parC mutation (Tyr57-->Ser or Ser80-->Arg), while those with double gyrA mutations were more resistant.
着者Ling JM, Chan EW, Lam AW, Cheng AF
期刊名称ANTIMICROBIAL AGENTS AND CHEMOTHERAPY
出版年份2003
月份11
日期1
卷号47
期次11
出版社AMER SOC MICROBIOLOGY
页次3567 - 3573
国际标準期刊号0066-4804
电子国际标準期刊号1098-6596
语言英式英语
Web of Science 学科类别Microbiology; MICROBIOLOGY; Pharmacology & Pharmacy; PHARMACOLOGY & PHARMACY
