Publication in refereed journal
香港中文大学研究人员 ( 现职)
| 彭智培教授 (眼科及视觉科学学系) |
| 林顺潮教授 (外科学系) |
| 蔡光伟教授 (妇产科学系) |
| 包立怡教授 (药剂学院) |
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引用次数
Web of Sciencehttp://aims.cuhk.edu.hk/converis/portal/Publication/17WOS source URL
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摘要High-throughput screening for single nucleotide polymorphisms (SNPs) or mutations can be achieved by inexpensive technologies. We modified the original protocols of conformation-sensitive gel electrophoresis (CSGE) to increase throughput several fold to 1.3 samples/min, which is about five times faster than denaturing high-performance liquid chromatography (DHPLC). The modifications include decreasing the gel thickness, increasing the number of lanes to 96, and increasing the number of samples per lane to seven. This high-throughput CSGE method is fast, robust, and as simple as the original protocols. Together with a two-stage strategy for screening homozygotes and the replacement of ethidium bromide with SYBR(R) Gold DNA dye staining, this protocol is a reliable and cost-effective alternative for laboratories that require high-throughput screening.
着者Leung YF, Tam POS, Tong WC, Baum L, Choy KW, Lam DSC, Pang CP
期刊名称Biotechniques
出版年份2001
月份2
日期1
卷号30
期次2
出版社EATON PUBLISHING CO
页次334 - +
国际标準期刊号0736-6205
语言英式英语
Web of Science 学科类别Biochemical Research Methods; BIOCHEMICAL RESEARCH METHODS; Biochemistry & Molecular Biology; BIOCHEMISTRY & MOLECULAR BIOLOGY
