Publication in refereed journal
香港中文大学研究人员 ( 现职)
| 韦妙宜教授 (那打素护理学院) |
| 张锦芳教授 (矫形外科及创伤学系) |
| 曾淑莹教授 (生命科学学院) |
| 徐国荣教授 (生物医学学院) |
| 温志昌教授 (生物医学学院) |
| 陆颖菲女士 (矫形外科及创伤学系) |
| 王言博士 (生物医学学院) |
| 梁伟铖博士 (生物医学学院) |
全文
| 数位物件识别号 (DOI) http://dx.doi.org/10.1002/jcb.25214 |
引用次数
Web of Sciencehttp://aims.cuhk.edu.hk/converis/portal/Publication/3WOS source URL
其它资讯
摘要In the past decade, miRNA emerges as a vital player in orchestrating gene regulation and maintaining cellular homeostasis. It is well documented that miRNA influences a variety of biological events, including embryogenesis, cell fate decision, and cellular differentiation. Adipogenesis is an organized process of cellular differentiation by which pre-adipocytes differentiate towards mature adipocytes. It has been shown that adipogenesis is tightly modulated by a number of transcription factors such as PPAR, KLF4, and C/EBP. However, the molecular mechanisms underlying the missing link between miRNA and adipogenesis-related transcription factors remain elusive. In this study, we unveiled that miR-25, a member of miR-106b-25 cluster, was remarkably downregulated during http://aims.cuhk.edu.hk/converis/portal/Publication/3Thttp://aims.cuhk.edu.hk/converis/portal/Publication/3-L1 adipogenesis. Restored expression of miR-25 significantly impaired http://aims.cuhk.edu.hk/converis/portal/Publication/3Thttp://aims.cuhk.edu.hk/converis/portal/Publication/3-L1 adipogenesis and downregulated the expression of serial adipogenesis-related genes. Further experiments presented that ectopic expression of miR-25 did not affect cell proliferation and cell cycle progression. Finally, KLF4 and C/EBP, two key regulators of adipocyte differentiation, were experimentally identified as bona fide targets for miR-25. These data indicate that miR-25 is a novel negative regulator of adipocyte differentiation and it suppressed http://aims.cuhk.edu.hk/converis/portal/Publication/3Thttp://aims.cuhk.edu.hk/converis/portal/Publication/3-L1 adipogenesis by targeting KLF4 and C/EBP, which provides novel insights into the molecular mechanism of miRNA-mediated cellular differentiation. J. Cell. Biochem. 116: 2658-2666, 2015. (c) 2015 Wiley Periodicals, Inc.
着者Liang WC, Wang Y, Liang PP, Pan XQ, Fu WM, Yeung VSY, Lu YF, Wan DCC, Tsui SKW, Tsang SY, Ma WB, Zhang JF, Waye MMY
期刊名称Journal of Cellular Biochemistry
详细描述To ORKTS: DOI: 10.1002/jcb.25214
出版年份2015
月份11
日期1
卷号116
期次11
出版社WILEY-BLACKWELL
页次2658 - 2666
国际标準期刊号07http://aims.cuhk.edu.hk/converis/portal/Publication/30-2http://aims.cuhk.edu.hk/converis/portal/Publication/312
电子国际标準期刊号1097-4644
语言英式英语
关键词http://aims.cuhk.edu.hk/converis/portal/Publication/3Thttp://aims.cuhk.edu.hk/converis/portal/Publication/3-L1; ADIPOGENESIS; MicroRNA; miR-25
Web of Science 学科类别Biochemistry & Molecular Biology; Cell Biology
