Publication in refereed journal
香港中文大学研究人员 ( 现职)
| 沈祖尧教授 (内科及药物治疗学系) |
| 朱妙婷女士 (微生物学系) |
| 吴浩强教授 (病理解剖及细胞学系) |
| 沈祖尧教授 (内科及药物治疗学系) |
| 唐红文博士 (病理解剖及细胞学系) |
| 朱妙婷女士 (微生物学系) |
| 张丽群博士 (那打素护理学院) |
| 罗?业教授 (病理解剖及细胞学系) |
| 杜家辉教授 (病理解剖及细胞学系) |
| 陈基湘教授 (微生物学系) |
全文
| 数位物件识别号 (DOI) http://dx.doi.org/10.1002/jmv.20138 |
引用次数
Web of Sciencehttp://aims.cuhk.edu.hk/converis/portal/Publication/38WOS source URL
其它资讯
摘要Severe acute respiratory syndrome coronavirus (SARS-CoV) can produce gastrointestinal symptoms. The intestinal tract is the only extrapulmonary site where viable viruses have been detected. This study examined seven established human intestinal cell lines, DLD-1, HICT-116, HT-29, LoVo, LS-180, SW-480 and SW-620, for their permissiveness to SARS-CoV infection. The results showed that only LoVo cells were permissive to SARS-CoV infection as evident by positive findings from indirect immunofluorescence staining for intracellular viral antigens, in situ hybridization for intracellular viral RNA, and electron microscopy for intracellular viral particles. In contrast to Vero cells, SARS-CoV did not produce cytopathic effects on LoVo cells. However, LoVo cells were found to be highly permissive for productive infection with a high viral titre (>3 x 10(7) viral copies/ml) produced in culture supernatant following a few days of incubation. SARS-CoV established a stable persistent chronic infection that could be maintained after multiple passages. Being a cell line of human origin LoVo cells could be a useful in vitro model for studying the biology and persistent infection of SARS-CoV. Our results on the expression of angiotensin-converting enzyme 2 (ACE2), a recently identified cellular receptor for SARS-CoV, in these cell lines indicated that it might not be the sole determinant for cells to be susceptible to SARS-CoV infection. (C) 2004 Wiley-Liss, Inc.
着者Chan PKS, To KF, Lo AWI, Cheung JLK, Chu I, Au FWL, Tong JHM, Tam JS, Sung JJY, Ng HK
期刊名称Journal of Medical Virology
出版年份2004
月份9
日期1
卷号74
期次1
出版社WILEY-LISS
页次1 - 7
国际标準期刊号0146-6615
电子国际标準期刊号1096-9071
语言英式英语
关键词ACE2; coronavirus; in vitro; persistent infection; receptor; SARS
Web of Science 学科类别Virology; VIROLOGY
