Publication in refereed journal
香港中文大学研究人员 ( 现职)
| 董钰娟博士 (外科学系) |
| 于君教授 (内科及药物治疗学系) |
| 吴兆文教授 (外科学系) |
| 梁荣华博士 (外科学系) |
| 康伟教授 (病理解剖及细胞学系) |
| 刘晓东博士 (麻醉及深切治疗学系) |
| 吴宗华博士 (内科及药物治疗学系) |
| 赵俊红博士 (内科及药物治疗学系) |
| 沈祖尧教授 (内科及药物治疗学系) |
| 陈家亮教授 (内科及药物治疗学系) |
| 张宁先生 (内科及药物治疗学系) |
全文
| 数位物件识别号 (DOI) http://dx.doi.org/10.1158/1541-7786.MCR-13-0061 |
引用次数
Web of Sciencehttp://aims.cuhk.edu.hk/converis/portal/Publication/41WOS source URL
其它资讯
摘要Dysregulated microRNA (miRNA) expression was profiled through a miRNA array comparison between human colorectal cancer tumors and their adjacent normal tissues. Specifically, using laser capture microdissection, miR-133a was shown to be significantly downregulated in primary colorectal cancer specimens compared with matched adjacent normal tissue. Ectopic expression of miR-133a significantly suppressed colorectal cancer cell growth in vitro and in vivo. Cell-cycle analysis revealed that miR-133a induced a G(0)/G(1)-phase arrest, concomitant with the upregulation of the key G(1)-phase regulator p21(Cip1). We further revealed that miR-133a markedly increased p53 protein and induced p21(Cip1) transcription. Studies in silico revealed that the 3'UTR of the ring finger and FYVE-like domain containing E3-ubiquitin protein ligase (RFFL), which regulates p53 protein, contains an evolutionarily conserved miR-133a binding site. miR-133a repressed RFFL-3'UTR reporter activity and reduced RFFL protein levels, indicating that miR-133a directly bound to RFFL mRNA and inhibited RFFL translation. Moreover, miR-133a sensitized colon cancer cells to doxorubicin and oxaliplatin by enhancing apoptosis and inhibiting cell proliferation. These data add weight to the significance of miR-133a in the development of CRC. (C) 2013 AACR.
着者Dong YJ, Zhao JH, Wu CW, Zhang LJ, Liu XD, Kang W, Leung WW, Zhang N, Chan FKL, Sung JJY, Ng SSM, Yu J
期刊名称Molecular Cancer Research
出版年份2013
月份9
日期1
卷号11
期次9
出版社American Association for Cancer Research
页次1051 - 1060
国际标準期刊号15http://aims.cuhk.edu.hk/converis/portal/Publication/41-7786
电子国际标準期刊号1557-3125
语言英式英语
Web of Science 学科类别Cell Biology; CELL BIOLOGY; Oncology; ONCOLOGY
