副教授,硕士生导师,岳麓****
E-mail:cylei@hnu.edu.cn




基本信息

教育背景
2008.9-2014.9 湖南大学 分析化学 理学博士
2004.9-2008.6湖南大学 生物技术 理学学士

工作履历
2020.01-至今 湖南大学化学化工学院 副教授，硕士生导师
2017.03-2019.12 湖南大学化学化工学院 助理教授，硕士生导师
2014.10-2017.03 浙江大学生工食品学院 助理研究员/博士后

学术兼职
担任Anal. Chem.,Adv. Sci, TrAC, Biosens. Bioelectron., Analyst, Adv.Healthc. Mater., Adv. Opt. Mater., Sensor. Actuat. B-Chem.等多个SCI期刊独立审稿人。
研究领域
采用合成生物学策略，设计和构建模块化生物分子识别、信号放大元件，发展高灵敏的生物传感与成像方法。

科研项目
在研国家自然科学基金面上项目和湖南省自然科学基金青年项目各1项，曾主持国家自然科学基金青年基金项目和中国博士后科学基金项目。
学术成果
主要研究方向为化学生物传感与成像，以第一作者和通讯作者身份在Angew. Chem. Int. Ed. (2),Chem. Sci. (1), Sci. ChinaChem.(1),Anal. Chem.(8)等国际国内化学学术期刊上发表20多篇SCI论文，h-index = 21 (googlescholar)。论文发表详情见ORCID:https://orcid.org/0000-0002-3078-5553.
部分研究论文：
F. Liu, R. Chen, W. Song, L. Li,C. Lei*, Z. Nie.Modular combination of proteolysis-responsive transcription and spherical nucleic acids for smartphone-based colorimetric detection of protease biomarkers.Anal. Chem., 2021, 93,3517–3525.
J. Zhao^＃,Z. Tan^＃, L. Wang*,C. Lei*, Z. Nie.A ligation-driven CRISPR-Cas biosensing platform for non-nucleic acid target detections. Chem. Commun., 2021,57, 7051–7054 .
K. Shi, L. Cao, F. Liu, S. Xie, S. Wang,Y. Huang,C. Lei*, Z. Nie.Amplified and label-free electrochemical detection of a protease biomarker by integrating proteolysis-triggered transcription.Biosens. Bioelectron.,2021, 190, 113372.
M. Yang^＃, K. Shi^＃, F. Liu, W. Kang,C. Lei*, Z. Nie*.Coupling of proteolysis-triggered transcription and CRISPR-Cas12a for ultrasensitive protease detection.Sci.China Chem.,2021,64, 330–336.
X. Luo, J. Zhao, X. Xie, F. Liu, P. Zeng,C. Lei*, Z. Nie.Proteolysis-responsive rolling circle transcription assay enablingfemtomolar sensitivity detection of a target protease biomarker.Anal. Chem., 2020, 92,16314–16321.

F. Liu, M. Yang, W. Song, X. Luo, R. Tang, Z. Duan, W. Kang, S. Xie, Q. Liu,C. Lei*, Y. Huang, Z. Nie*, S. Yao.Target-activated transcription for the amplified sensing of protease biomarkers. Chem. Sci., 2020, 11, 2993–2998.
S. Wang, P. Zeng, X. Zhu,C. Lei*, Y. Huang, Z. Nie*.Chimeric peptides self-assembling on titanium carbide MXenes as biosensing interfaces for activity assay of post-translational modification enzymes.Anal. Chem., 2020, 92,8819–8826.
H. Deng, H. Liu, W. Kang,C. Lei*, Z. Nie, Y. Huang*, S. Yao.Biomineralization synthesis of a near-infrared fluorescent nanoprobe for direct glucose sensing in whole blood.Nanoscale, 2020, 12, 864–870.

G. Feng^＃, X. Luo^＃, X. Lu, S. Xie, L. Deng, W. Kang, F. He, J. Zhang,C. Lei*, B, Lin, Y. Huang, Z. Nie*, S. Yao.Engineering of nucleic acids and synthetic cofactors as holo sensors for probing signaling molecules in the cellular membrane microenvironment.Angew. Chem. Int. Ed., 2019, 58, 6590?6594. (Very Important Paper, Back Cover)
S. Wang, W. Song, S. Wei, S. Zeng, S. Yang,C. Lei*, Y. Huang, Z. Nie*, S. Yao. Functional titanium carbide MXenes-loaded entropy-driven RNA explorer for long noncoding RNA PCA3 imaging in live cells.Anal. Chem., 2019, 91,8622?8629.
S. Wang, S. Wei, S. Wang, X. Zhu,C. Lei*, Y. Huang, Z. Nie*, S. Yao.Chimeric DNA-functionalized titanium carbide MXenes for simultaneous mapping of dual cancer biomarkers in living cells.Anal. Chem., 2019,91,1651?1658.
Z. Guo, X. Zhu, S.Wang,C. Lei*, Y. Huang, Z. Nie, S. Yao.Fluorescent Ti₃C₂MXene quantum dots for an alkaline phosphatase assay and embryonic stem cell identification based on the inner filter effect.Nanoscale, 2018, 10, 19579–19585.
X. Zhu, L. Fan, S. Wang,C. Lei*, Y. Huang, Z. Nie*, S. Yao.Phospholipid-tailored titanium carbide nanosheets as a novel fluorescent nanoprobe for activity assay and imaging of phospholipase D.Anal. Chem., 2018, 90, 6742?6748.
C. Lei, H. Dai, Y. Fu*, Y. Ying, Y. Li*. Colorimetric sensor array for thiols discrimination based on urease-metal ion pairs.Anal. Chem., 2016, 88, 8542?8547.
C. Lei, Z. Wang, Z. Nie, H. Deng, H. Hu, Y. Huang*, S. Yao. Resurfaced fluorescent protein as a sensing platform for label-free detection of copper(II) ion and acetylcholinesterase activity.Anal. Chem., 2015, 87, 1974?1980.
C. Lei, Y. Huang, Z. Nie*, J. Hu, L. Li, G. Lu, Y. Han, S. Yao. Supercharged fluorescent protein as a versatile probe for homogenous DNA detection and methylation analysis.Angew. Chem. Int. Ed., 2014, 53, 8358–8362. (Hot Paper)