博士，教授，博士生导师
E-mail: jinhuang@hnu.edu.cn




基本信息
化学生物传感与计量学国家重点实验室，生物纳米与分子工程湖南省重点实验室，湖南大学化学化工学院教授，博士生导师，湖南大学岳麓****，湖南省****基金获得者，教育部青年****，主要研究领域为生物分析化学。迄今以第一作者/通讯作者在JACS, Angewandte Chemie, ACS Nano, Chemical Science, Analytical Chemistry, Chemical Communications等国际学术期刊上发表论文50余篇，署名论文100余篇，论文被引用3000多次，H-index35。先后主持国家和省部级科研项目10余项。任湖南省化学化工学会第十二届理事会理事，JAT青年编委等。




教育背景
2009-2011：美国佛罗里达大学 联合培养博士
2005-2011：湖南大学化学化工学院 理学博士

2001-2005：湖南大学化学化工学院 理学学士

工作履历
2019-至今：湖南大学化学化工学院 教授
2015-2019：湖南大学化学化工学院 副教授
2012-2015：湖南大学化学化工学院 助理教授
2011-2017：湖南大学化学化工学院 博士后


学术兼职
1. 湖南省化学化工学会第十二届理事会理事（2019-2023）
2. 《Journal of Analysis and Testing》青年编委（2021-2022）
研究领域
生物分析化学/荧光核酸探针
科研项目
主持
国家自然科学基金面上项目，**，“双控制、双放大”脱氧核酶级联电路及在精准分子诊断中的应用，2019/1-2022/12
国家自然科学基金青年项目，**，“开关型”荧光纳米探针用于活细胞内生物分子检测，2013/1-2015/12
湖南省****基金项目（已公示），活细胞内原位荧光成像，2021/1-2023/12
湖南省自然科学基金面上项目，2017JJ2039，核酸信号放大技术用于活细胞内生物分子在线检测，2017/1-2019/12
湖南省自然科学基金青年项目，13JJ4032，结合杂交链式反应和光学纳米材料构建高灵敏的核酸检测平台，2013/1-2015/12
中国博士后面上项目，2013M531779，结合杂交链式反应和氧化石墨烯构建高灵敏的核酸检测平台，2013/1-2014/12
生物反应器工程国家重点实验室开放课题基金项目，基于i-motif的荧光pH探针用于活细胞成像研究，2014/6-2016/6
化学生物传感与计量学国家重点实验室优秀青年人才培养计划项目,2019/1-2019/12
化学生物传感与计量学国家重点实验室交叉学科前沿研究项目，2020/7-2022/7
湖南大学优秀青年人才培育计划项目，2018/1-2018/12
普通高校青年教师成长计划项目,2013/1-2017/12


参与
国家自然科学基金重大项目子课题，**，核酸适配分子成像与靶向定位，2012/1-2016/12
国家自然科学基金重点项目，**，基于RNA疾病标志物的精准诊疗研究，2018/1-2022/12
学术成果
以第一作者或通讯作者身份在国际学术期刊上发表研究论文列表（按时间倒序排列如下）：
2021年：
[52]Lin Lan+,Jin Huang+,Mengtan Liu, Yao Yin, Can Wei, Qinyun Cai*, Xiangxian Meng*. Polymerization and isomerization cyclic amplification for nucleic acid detection with attomolar sensitivity.Chemical Science, 2021, 12, 4509-4518.
[51] Jing Li+, Anmin Wang+, Xiaohai Yang, Kemin Wang, Jin Huang*. Orderly-assembled, self-powered FRET flares for microRNA imaging in live cells. Analytical Chemistry, 2021, 93, 6270-6277.
[50] Qing Lin, Shijun Cai, Bing Zhou, Kemin Wang, Lixin Jian*, Jin Huang*. Dual-microRNA-regulation of singlet oxygen generation by DNA-tetrahedron-based molecular logic device. Chemical Communications, 2021, 57, 3873-3876.
[49] Shijun Cai, Jiaoli Wang, Jing Li, Bing Zhou, Chunmei He, Xiangxian Meng*, Jin Huang*, Kemin Wang. A self-assembled DNA nanostructure as a FRET nanoflare for intracellular ATP imaging.Chemical Communications, 2021,57, 6257-6260.
[48] Yehua Liu, Zhenxiang Liu, Ruiting Liu, Kemin Wang, Hui Shi*, Jin Huang*. A MnO₂ nanosheet-mediated photo-controlled DNAzyme for intracellular miRNA cleavage to suppress cell growth. Analyst, 2021, 146, 3391-3398.
2010-2020年：
[47]Yanjing Yang, Yao He, Zhiwei Deng, Jiacheng Li, Xiufang Li,Jin Huang*, Shian Zhong*. An autonomous self-cleavage DNAzyme walker for live cell microRNA imaging. ACS Applied Bio Materials, 2020, 3, 6310-6318.
[46] Yanjing Yang, Yao He, Zhiwei Deng, Jiacheng Li, Jin Huang*, Shian Zhong*. Intelligent nanoprobe: acid-responsive drug release and in situ evaluation of its own therapeutic effect. Analytical Chemistry, 2020, 92, 12371-12378.
[45] Jing Li, Jiaoli Wang, Shiyuan Liu, Nuli Xie, Ke Quan, Yanjing Yang, Xiaohai Yang, Jin Huang*, Kemin Wang*. Amplified FRET nanoflare: An endogenous mRNA-powered nanomachine for intracellular microRNA imaging. Angewandte Chemie International Edition, 2020,59,20104-20111.
[44] Nuli Xie, Haiyan Wang, Ke Quan, Feng Feng*, Jin Huang*, Kemin Wang*. Self-assembled DNA-based geometric polyhedrons: construction and applications. Trends in Analytical Chemistry, 2020, 126, 115844.
[43] Ke Quan, Cuiping Yi, Xiaohai Yang, Xiaoxiao He, Jin Huang*, Kemin Wang*. FRET-based nucleic acid probes: basic designs and applications in bioimaging. Trends in Analytical Chemistry, 2020, 124, 115784.
[42] Jiaoli Wang, Jing Li, Shiyuan Liu, Xiangxian Meng, Xiaohai Yang, Jin Huang*, Kemin Wang*. An amplified AND logic platform for cell identification. Chemical Communications, 2020, 56, 11267-11270.
[41] Yanan Wu, Jing Li, Ke Quan, Xiangxian Meng, Qiuping Guo, Xiaohai Yang, Jin Huang*, Kemin Wang*. DNAzyme cascade for amplified detection of intracellular miRNA. Chemical Communications, 2020, 56, 10163-10166.
[40] Anmin Wang, Qing Lin, Shiyuan Liu, Jing Li, Jiaoli Wang, Ke Quan, Xiaohai Yang, Jin Huang*, Kemin Wang. Aptamer-tethered self-assembled FRET-flares for micrRNA imaging in living cancer cells. Chemical Communications, 2020, 56, 2463-2466.
[39] Qing Lin, Anmin Wang, Shiyuan Liu, Jing Li, Jiaoli Wang, Ke Quan, Xiaohai Yang, Jin Huang*, Kemin Wang. A DNA-tetrahedron-based molecular computation device for logic sensing of dual microRNAs in living cells. Chemical Communications, 2020, 56, 5303-5306.
[38] Jing Li, Shijun Cai, Bing Zhou, Xiangxian Meng, Qiuping Guo, Xiaohai Yang, Jin Huang*, Kemin Wang*. Photocaged FRET nanoflares for intracelluar microRNA imaging. Chemical Communications, 2020,56, 6126-6129.
[37] Jing Li, Ke Quan, Yanjing Yang, Xiaohai Yang, Xiangxian Meng, Jin Huang*, Kemin Wang*. Engineering DNAzyme cascade for signal transduction and amplification. Analyst, 2020, 145, 1925-1932.
[36] Nuli Xie, Shiyuan Liu, Hongmei Fang, Yanjing Yang, Ke Quan, Jing Li, Xiaohai Yang, Kemin Wang, Jin Huang*. Three-dimensional molecular transfer from DNA nanocages to inner gold nanoparticle surfaces. ACS Nano, 2019, 13, 4174-4182.
[35] Ke Quan, Jing Li, Jiaoli Wang, Nuli Xie, Qiaomei Wei, Jinlu Tang, Xiaohai Yang, Kemin Wang, Jin Huang*. Dual-microRNA-controlled double-amplified cascaded logic circuits for accurate discrimination of cell subtypes. Chemical Science, 2019, 10, 1442-1449.
[34]Lin Lan, Qiuping Guo, Hemin Nie, Chang Zhou, Qingyun Cai, Jin Huang*, Xiangxian Meng*. Linear-hairpin variable primer RT-qPCR for microRNA. Chemical Science, 2019, 10, 2034-2043.
[33] Yanjing Yang, Shian Zhong, Kemin Wang, Jin Huang*. Gold nanoparticle based fluorescent oligonucleotide probes for imaging and therapy in living systems. Analyst, 2019, 144, 1052-1072.
[32]Qiaomei Wei,Jin Huang*,Jing Li, Jiaoli Wang, Xiaohai Yang, Jianbo Liu, Kemin Wang. DNA nanowire based localized catalytic hairpin assembly reaction for microRNA imaging in live cells.Chemical Science, 2018, 9, 7802-7808.
[31]Jiaoli Wang,Jin Huang*, Ke Quan, Jing Li, Yanan Wu, Qiaomei Wei, Xiaohai Yang, Kemin Wang*. Hairpin-fuelled catalytic nanobeacons for amplified microRNA imaging in live cells.Chemical Communications, 2018, 54, 10336-10339.
[30]Min Ou,Jin Huang*, Xiaohai Yang, Xiaoxiao He, Ke Quan, Yanjing Yang, Nuli Xie, Jing Li, Kemin Wang*. Live cell microRNA imaging via MnO2nanosheets mediated “DD-A” hybridization chain reaction.ChemBioChem, 2018, 19, 147-152.
[29]Jing Li,Jin Huang*, Xiaohai Yang, Yanjing Yang, Ke Quan, Nuli Xie, Yanan Wu, Changbei Ma, Kemin Wang*. Two-color-based nanoflares for multiplexed microRNAs imaging in live cells.Nanotheranostics, 2018, 2, 96-105.
[28]Jing Li,Jin Huang*, Xiaohai Yang, Yanjing Yang, Ke Quan, Nuli Xie, Yanan Wu, Changbei Ma, Kemin Wang*. Gold nanoparticle-based 2’-O-methyl modified DNA probes for breast cancerous theranostics.Talanta, 2018, 183, 11-17.
[27]Min Ou, Jin Huang*, Xiaohai Yang, Ke Quan, Yanjing Yang, Nuli Xie, Kemin Wang*. MnO₂nanosheets mediated “DD-A” FRET binary probes for sensitive detection of intracellular mRNA. Chemical Science, 2017, 8, 668-672.
[26]Yangjin Yang, Jin Huang*, Xiaohai Yang, Xiaoxiao He, Ke Quan, Nuli Xie, Min Ou, Kemin Wang*. Gold nanoparticle based hairpin-locked-DNAzyme probe for amplified miRNA imaging in living cells. Analytical Chemistry, 2017, 89, 5850-5856.
[25]Yanan Wu, Jin Huang*, Xiaohai Yang, Yanjing Yang, Ke Quan, Nuli Xie, Jing Li, Changbei Ma, Kemin Wang*. Gold nanoparticle loaded split-DNAzyme probe for amplified miRNA detection in living cells. Analytical Chemistry, 2017, 89, 8377-8383.
[24]Nuli Xie, Jin Huang*, Xiaohai Yang, Xiaoxiao He, Jianbo Liu, Jiaqi Huang, Hongmei Fang, Kemin Wang*. Scallop-inspired DNA nanomachine: A ratiometric nanothermometer for intracellular temperature sensing. Analytical Chemistry, 2017, 89, 12115-12122.
[23] Nuli Xie, Shiyuan Liu, Xiaohai Yang, Xiaoxiao He,Jin Huang*,Kemin Wang*.DNA tetrahedron nanostructures for biological applications: biosensors and drug delivery. Analyst, 2017, 142, 3322-3332.
[22]Jin Huang, He Wang, Xiaohai Yang, Ke Quan, Yanjing Yang, Le Ying, Nuli Xie, Min Ou, Kemin Wang*. Fluorescence resonance energy transfer-based hybridization chain reaction for in situ visualization of tumor-related mRNA. Chemical Science, 2016, 7, 3829-3835.
[21]Ke Quan, Jin Huang*, Xiaohai Yang, Yanjing Yang, Le Ying, He Wang, Nuli Xie, Min Ou, Kemin Wang*. Powerful amplification cascades of FRET-based two-layer nonenzymatic nucleic acid circuits. Analytical Chemistry, 2016, 88, 5857-5864.
[20]Yanjing Yang, Jin Huang*, Xiaohai Yang, Ke Quan, He Wang, Le Ying, Nuli Xie, Min Ou, Kemin Wang*. Aptazyme-gold nanoparticle sensor for amplified molecular probing in living cells. Analytical Chemistry, 2016, 88, 5981-5987.
[19]Yanjing Yang, Jin Huang*, Xiaohai Yang, Ke Quan, Nuli Xie, Min Ou, Jinglu Tang, Kemin Wang*.Aptamer-based FRET nanoflares for imaging potassium ions in living cells. Chemical Communications,2016, 52, 11386-11389.
[18]Jin Huang, He Wang, Xiaohai Yang, Yanjing Yang, Ke Quan, Le Ying, Nuli Xie, Min Ou, Kemin Wang*. A supersandwich fluorescence in situ hybridization strategy for highly sensitive and selective mRNA imaging in tumor cells. Chemical Communications,2016, 52, 370-373.
[17]Nuli Xie, Jin Huang*, Xiaohai Yang, Yanjing Yang, Ke Quan, He Wang, Le Ying, Min Ou, Kemin Wang*. DNA tetrahedron-based molecular beacon for tumor-related mRNA detection in living cells. Chemical Communications,2016, 52, 2346-2349.
[16] Le Ying, Nuli Xie, Yanjing Yang, Xiaohai Yang, Qifeng Zhou, Bincheng Yin, Jin Huang*, Kemin Wang*. A cell-surface-anchored ratiometric i-motif sensor for extracellular pH detection. Chemical Communications,2016, 52, 7818-7821.
[15]Nuli Xie,Jin Huang*, Xiaohai Yang, Yanjing Yang, Ke Quan, Min Ou, Hongmei Fang, Kemin Wang*.Competition-mediated FRET-switching DNA tetrahedron molecular beacon for intracellular molecular detection. ACSsensors, 2016, 1, 1445-1452.
[14]Yanjing Yang+, Jin Huang+, Xiaohai Yang, Ke Quan, He Wang, Le Ying, Nuli Xie, Min Ou, Kemin Wang*. FRET nanoflares for intracellular mRNA detection: avoiding false positive signals and minimizing effects of system fluctuations. Journal of the American Chemical Society, 2015,137, 8340-8343.
[13] Ke Quan+, Jin Huang+, Xiaohai Yang, Yanjing Yang, Le Ying, He Wang, Yong He, Kemin Wang*. An enzyme-free and amplified colorimetric detection strategy via target-aptamer binding triggered catalyzed hairpin assembly. Chemical Communications,2015, 51, 937-940.
[12]Ke Quan+, Jin Huang+, Xiaohai Yang, Yanjing Yang, Le Ying, He Wang, Yong He, Kemin Wang*.An enzyme-free and amplified colorimetric detection strategy: assembly of gold nanoparticles through target-catalytic circuits. Analyst, 2015, 140, 1004-1007.
[11]Jin Huang, Le Ying, Xiaohai Yang, Yanjing Yang, Ke Quan, He Wang, Nuli Xie, Min Ou, Qifeng Zhou, Kemin Wang*. Ratiometric fluorescent sensing of pH values in living cells by dual-fluorophore-labeled i-motif nanoprobes. Analytical Chemistry, 2015, 87, 8724-8731.
[10] Jin Huang, Xiaohai Yang, Xiaoxiao He, Kemin Wang*, Jianbo Liu, Hui Shi, Qing Wang, Qiuping Guo, Dinggeng He. Design and bioanalytical applications of DNA hairpin-based fluorescent probes. Trends in Analytical Chemistry, 2014, 53, 11-20.
[9] Jin Huang, Yong He, Xiaohai Yang, Kemin Wang*, Le Ying, Ke Quan, Yanjing Yang, Bincheng Yin. I-motif-based nano-flares for sensing pH changes in live cells. Chemical Communications, 2014, 50, 15768-15771.
[8]Jin Huang, Yong He, Xiaohai Yang, Kemin Wang*, Ke Quan, Xiaoping Lin. Splitaptazyme-based molecular beacons for amplified detection of adenosine. Analyst, 2014, 139, 2994-2997.
[7]Jin Huang, Yong He, Xiaohai Yang, Ke Quan, Kemin Wang*. Inhibited aptazyme-based catalytic molecular beacon for amplified detection of adenosine.Chinese Chemical Letters, 2014, 25, 1211-1214.
[6]Jin Huang, Xiaohai Yang, Xiaoxiao He, Kemin Wang*, Yong He, Ke Quan. A new strategy for designing graphene oxide-based DNA hairpin probe: fluorescence upon switching the orientation of sticky end.Chemical Communications, 2013, 49, 9827-9829.

[5]Kemin Wang*,Jin Huang, Xiaohai Yang, Xiaoxiao He, Jianbo Liu. Recent advances in fluorescent nucleic acid probes for living cell studies.Analyst, 2013, 138, 62-71.
[4]Jin Huang, Yanrong Wu, Yan Chen, Zhi Zhu, Xiaohai Yang, Chaoyong James Yang*, Kemin Wang*, Weihong Tan*. Pyrene-excimer probes based on the hybridization chain reaction for the detection of nucleic acids in complex biological fluids. Angewandte Chemie International Edition,2011, 50, 401-404.
[3]Jin Huang, Yan Chen, Liu Yang, Zhi Zhu, Guizhi Zhu, Xiaohai Yang, Kemin Wang*, Weihong Tan*. Amplified detection of cocaine based on strand displacement polymerization reaction and fluorescence resonance energy transfer.Biosensors and Bioelectronics, 2011, 28, 450-453.
[2]Xiaohai Yang+, Jin Huang+, Kemin Wang*, wei Li, Liang Cui, Xiaoping Li. Angiogenin-mediated photosensitizer-aptamer conjugate for photodynamic therapy. ChemMedChem, 2011, 6, 1778-1780.
[1] Jin Huang, Zhi Zhu, Suwussa Bamrungsap, Guizhi Zhu, Mingxu You, Xiaoxiao He, Keming Wang*, Weihong Tan*. Competition-mediated pyrene-switching aptasensor: probing lysozyme in human serum with a monomer-excimer fluorescence switch.Analytical Chemistry,2010, 82, 10158-10163.

奖励与荣誉
一、指导学生获奖情况
（1）学生毕业论文评优情况：
1. 指导应乐的毕业论文《基于荧光共振能量转移的i-motif探针用于细胞内外PH值检测》被评为湖南省优秀硕士毕业论文（2018）。
2. 指导欧敏的毕业论文《MnO2纳米片介导的“DD-A”FRET核酸探针用于细胞内RNA检测》被评为湖南省优秀硕士毕业论文（2019）。
3. 指导杨艳婧的毕业论文《基于金纳米颗粒的荧光核酸探针用于细胞内传感》被评为湖南省优秀博士毕业论文（2019）。

（2）学生奖学金获评情况：
2015年：博士生杨艳婧获国家奖学金和校奖学金；博士生全珂获校奖学金；硕士生应乐获国家奖学金。
2016年：博士生全珂获国家奖学金和校奖学金；博士生谢努力获校奖学金；硕士生欧敏获国家奖学金。
2017年：博士生杨艳婧获校奖学金；硕士生吴亚南获国家奖学金。
2018年：博士生谢努力获国家奖学金；硕士生韦巧梅获国家奖学金。
二、个人获奖情况
2020年入选教育部****奖励计划（青年****）