曾佑林

性 别：男

学历：博士

职称：教授

其他：博士生导师(化学)；硕士生导师(有机化学、学科教学(化学))




Youlin Zeng
Professor
Professional Background:
B.S., 1994, Hunan Normal University, Major: Chemistry.
M.S., 1998, Major: Polymer Chemistry and Physics.
Ph.D., 2001, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Major: Carbohydrte Chemistry.
Work Experience, 2004-2006, Hunan Normal University, Organic Chemistry Teaching & Science Research.
Postdoctoral Fellow, 2006-, The University of Toledo
Email: youlinzengcn@gmail.com Phone:(86) **
Office: New Chemical Engineering Building Room 528 Fax:(86)
Skype:z-y-l-cn QQ:
Research Synopsis:
Chemistry and biology of carbohydrates. Glycobiology is the last important frontier in biochemistry. The difficulty for carbohydrate research is caused by the complexity and variety of carbohydrate structures. Carbohydrate molecules become the biomolecules carrying the largest amounts of bio-information. As information carriers, carbohydrates play essential roles in living organisms, including determination of antigenecity of molecules and phenotype of cell, and recognition in many physiological and pathological events. The simulative syntheses of the bioactive oligosaccahrides for verifying important conclusions of bio-functions of natural oligosaccharides and further chemical modification for obtaining more active non-natural oligosaccahrides are very important for study on structure-bio-function relationships.
Gene delivery system The key technology for gene therapy is to design an effective and safe vector for targeted gene delivery. Cationic liposome based non-viral gene vectors are designed, synthesized and evaluated by in vitro transfection. The transfection properties and targeted assay of those gene vectors will be discovered by observing enhanced green fluorescent protein (EGFP) expression from pEGFP-C1 plasmid in HEK293, 293FT, GES-1, GC-1, SW-480, HT-1080, U251, 3T3-L1, MCF-7, Hct-116, HUVEC, HeLa, A549, HBL100-SV40 and NIH3T3 cells. And a structure-activity relationship based on the chemical structure and physical structure of lipids gene delivery vectors will be established by evaluating the effects of the chemical structure and physical structure such as length of hydrophobic chain, length of the linkage between hydrophilic terminal and hydrophobic terminal, hydrophilic terminal types, hydrophilic terminal density, surface zeta potential, particle size and size distribution, on the transfection properties. This structure–activity relationship will be to provide a guiding theory for constructing new cationic liposome-based non-viral targeted gene delivery vectors and their application.
基金与项目
主持项目情况：
1. 环二核苷酸阳离子脂质体载体系统的构建及其免疫刺激活性研究(国家自然科学基金面上项目，直接经费67万元，**，2019.01-2022.12，主持人)； 2.利拉鲁肽长效化研究(动物多肽药物创制国家地方联合工程实验室开放基金，2017KF003，5.0万元，2018.01-2019.12，主持人)；3. 阳离子脂质体的产业化及其在药物中的应用研究(厦门市“双百计划”领军创业人才项目, 2017.01-2019.12, >100万元,主持人)；4.新型糖类脂质体靶向基因载体的设计、合成及转染性能研究(国家自然科学基金面目项目，80万元，**，2013.01-2016.12，主持人)；
5.抗流感病毒的唾液酸类似物的合成以及方法学研究(教育部“留学回国人员科研启动基金”外教司留[2010]609号-03，3.5万元，2011.01-2013.12，主持人)；
6. 以糖基磺酸酯为糖苷化供体合成天麻素及其类似物(湖南省教育厅科学研究基金，1.0万元，No.2006，2007.01.-2008.01，主持人)；
7. 几种多糖的分离纯化与构效关系研究(湖南师范大学博士启动基金，2.5万元，化050603，2004.12.-2006.12.，主持人)；
8. 几种氨基酸双保护合成工艺开发(横向，5万元，化080427，2008.09~2009.9，主持人)
参与项目情况：
1. 抗肿瘤寡核苷酸类药物及其体内靶向载体的研发与应用("863计划"资源环境技术领域2007年度专题课题，2007AA02Z172，2008-2010，300万元，组长为张健教授，副组长苏胜培教授，主要参与人)
2. Development of New Carbohydrate Synthesis Methodologies (NIH, $1,194,834, R01 GM72667, PI: Xuefei Huang)
3. 微波化学法处理砷镉重金属废水机理研究(所属课题：湘江水环境重金属污染整治关键技术研究与综合示范，子课题：水口山复合重金属污染控制技术与集成，编号：2009ZX07212 -001-04，主持单位：湖南省环境保护科学研究院，主要参与人)
5.抗流感病毒的唾液酸类似物的合成以及方法学研究(教育部“留学回国人员科研启动基金”外教司留[2010]609号-03，3.5万元，2011.01-2013.12，主持人)； 6. 以糖基磺酸酯为糖苷化供体合成天麻素及其类似物(湖南省教育厅科学研究基金，1.0万元，No.2006，2007.01.-2008.01，主持人)；7. 几种多糖的分离纯化与构效关系研究(湖南师范大学博士启动基金，2.5万元，化050603，2004.12.-2006.12.，主持人)；8. 几种氨基酸双保护合成工艺开发(横向，5万元，化080427，2008.09~2009.9，主持人)
Publications
1. Cui Jian, Peng Zhang, Jing Ma, Shandong Jian,Qianqian Zhang, Bobo Liu, Songping Liang, Meiyan Liu, Youlin Zeng, Zhonghua Liu.The Roles of Fatty-Acid Modification in the Activity of the Anticancer PeptideR-Lycosin-I.Mol. Pharmaceutics, 2018, 15 (10), pp4612–4620.2. PengZhang, Jing Ma, Yujie Yan, Bo Chen, Bobo Liu, Cui Jian, Baode Zhu, Song pingLiang, Youlin Zeng, Zhonghua Liu. Arginine Modification of Lycosin-I to ImproveInhibitory Activity Against Cancer Cells. Org. Biomol. Chem.,2017, 15(44), 9379-9388.3. Bo Li, WanrongGuo, Fan Zhang, Meiyan Liu, Shang Wang, Zhonghua Liu, Shuanglin Xiang, YoulinZeng. Synthesis and evaluation ofL-arabinosebased cationic glycolipids as effective vectors for pDNA and siRNAinvitro. PLOS ONE, 12(7), e**(July 3,2017)4. Bo Li,Liangliang Deng, Meiyan Liu, Youlin Zeng.Evaluation of maltose-based cationic liposomes withdifferent hydrophobic tails for plasmid DNA delivery.Molecules.2017, 22(3), 406.5. ChengxiHe, Shang Wang, Meiyan Liu, Chunyan Zhao, Shuanglin Xiang and Youlin Zeng*, Design,synthesis and in vitro evaluation of D-glucose-based cationic glycolipids forgene delivery. Org. Biomol. Chem.,2016, 14(5), 1611–1622.6. 刘美艳,曾佑林*.甲基2,3,4,6-四-O-苯甲酰基-a-D-吡喃甘露糖苷的合成及结构表征—推荐一个综合药物合成实验.大学化学,2016, 31(4), 60-66.7. Yan Long, Jialing Shi, Haoqiang Zeng, Youlin Zeng, Qian Cai. Enantioselective Formation of Cyano-Bearing All-Carbon Quaternary Stereocenters: Copper-Catalyzed N-Arylation via Kinetic Resolution. Synthesis, 2015, 47(18), 2844-2850.8. Xu Yan, Jinxi Liao, Yongzhi Lu, Jinsong Liu, Youlin Zeng, and Qian Cai, Pd-Catalyzed One-Pot Synthesis of Polysubstituted Acrylamidines from Isocyanides, Diazo Compounds, and Imines. Org. Lett., 2013, 15, 2478-2481. 9. 史明明, 刘美艳, 曾佑林, 苏胜培, 成应向. 硅藻土和膨润土对重金属离子Zn²⁺、Pb²⁺及Cd²⁺的吸附特性. 环境化学, 2012, 31, 162-167. 10. 吴烟苗, 曾佑林, 谢祥林, 徐满才, 低档茶叶中β-胡萝卜素的提取及纯化工艺研究, 中国资源综合利用, 2010, 28, 21-24. 11. Gilbert Wasonga, YouLin Zeng, XueFei Huang, Pre-activation based stereoselective glycosylations: Stereochemical control by additives and solvent. Sci. China Chem., 2011, 54, 66-73. 12. 氨基酸阳离子脂质体纳米颗粒的制备方法, 中国专利, 授权号: 8.9 13. Youlin Zeng, Zhen Wang, Dennis Whitfield, Xuefei Huang, Installation of Electron-Donating Protective Groups, a Strategy for Glycosylating Unreactive Thioglycosyl Acceptors using the Preactivation-Based Glycosylation Method. J.Org.Chem., 2008, 73, 7952-7962. 14. Adeline Miermont§, Youlin Zeng§, Yuqing Jing, Xin-shan Ye, Xuefei Huang, Syntheses of LewisX and Dimeric LewisX: Construction of Branched Oligosaccharides by a Combination of Preactivation and Reactivity Based Chemoselective One-Pot Glycosylations, J. Org. Chem., 2007, 72, 8958-8961. 15. 梁源, 周庆美, 曾佑林*, 徐满才, 谢青季, 葡萄糖酶电极测定环糊精聚合物中的环糊精单元, 高分子学报, 2007, 11, 1017-1021. 16. 曾佑林*, 孔繁祚, β-甘露糖苷和 β-氨基甘露糖苷的合成, 化学进展, 2006, 18, 907-926. 17. ZengYou-lin, Zhang, Jian-jun, Kong, Fan-zuo, Synthesis of Analogues of the Antiumor (1→6)-Branched (1→3)-Glucohexaose, Chin. J. Chem., 2004, 22, 460-466. 18. Youlin Zeng, Fanzuo Kong, Synthesis of a hexasaccharide fragment of group E streptococci polysaccharide and the tetrasaccharide repeating unit of E. coli O7:K98:H6, Carbohydr. Res., 2004, 339, 1503-1510. 19. Youlin Zeng, Jianjun Zhang, Jun Ning and Fanzuo Kong, Synthesis of a mannose heptasaccharide existing in baker's yeast, Saccharomyces cerevisiae X2180-1A wild-type strain, Carbohydr. Res., 2003, 338, 5-9. 20. Youlin Zeng, Fanzuo Kong, Synthesis of β-D-glucose oligosaccharides from Phytophthora parasitica, Carbohydr. Res., 2003, 338, 2359-2366. 21. Youlin Zeng, Fanzuo Kong, Synthesis of 3,6-Branched β-D-Glucose Oligosaccharides, J. Carbohydr. Chem., 2003, 22, 881-890. 22. Youlin Zeng, Jianjun Zhang, Fanzuo Kong, Synthesis of a mannose heptasaccharide of the pathogenic yeast, Candida glabrata IFO 0622 strain, Carbohydr. Res., 2002, 337, 1367-1371. 23. 曾佑林, 徐满才, 黄明刚, 一种新型亲水性胺基羧酸树脂的合成及其性能研究, 离子交换与吸附, 2002, 18, 272-276. 24. 曾佑林, 徐满才, 朱爱玲, 曾盈, 凝胶型交联聚苯乙烯-异氰尿酸树脂对酚类物质的吸附机理研究, 离子交换与吸附, 2001, 17, 110-116. 25. 陈如意, 黄幼援, 曾佑林, 苏胜培, 聚丙烯/碳酸钙复合材料的组分分析, 精细化工中间体, 2006, 36, 68-70.26. 丙二醇胺衍生物阳离子脂质体纳米颗粒的制备方法, 中国专利, 授权号: 9.5. 27. 曾佑林，何成喜，刘美艳,葡萄糖衍生物阳离子脂质体纳米颗粒的制备方法，发明专利，申请号20**，2014年10月20日。28. 曾佑林，彭嘉勋，刘美艳,氨基葡萄糖衍生物阳离子脂质体纳米颗粒的制备方法，中国发明专利，申请号20**，授权公告号CN B，授权公告日2018.08.24。29. 曾佑林，邓亮亮，刘美艳,甘露糖衍生物阳离子脂质体纳米颗粒的制备方法，发明专利，申请号20**，2014年10月20日。30. 曾佑林，郭婉蓉，刘美艳,半乳糖衍生物阳离子脂质体纳米颗粒的制备方法，发明专利，申请号20**，2015年6月01日。31.曾佑林、张翻、刘美艳，两类阿比特龙衍生物的合成，申请号20**，2017年4月21日。
Honors and Awards
2nd class scholarship of Hunan Normal University (1996, 1997) (awarded to 10% top students every year)
3rd class award of RCEES (2002-2003; 2003-2004)
3rd class award of Mathematical Contest in Modeling (China, 1997)
Personal Website: http://youlinzengcn.googlepages.com/home

近年来发表论文：

论文题目
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1
Carbohydr. Res.
/UPwork/063265.pdf

2
Carbohydr. Res.
/UPwork/****.pdf

3
Carbohydr. Res.
/UPwork/****.pdf

4
J. Carbohydr. Chem.


5
Chin. J. Chem.
/UPwork/****.pdf

6
Carbohydr. Res
/UPwork/****.pdf

7
Ion Exchange and Absorption
/UPwork/****.pdf

8
Ion Exchange and Absorption
/UPwork/****.pdf

9
Progress in Chemistry (Chinese)


10
Acta polymerica Sinica