孔庆然
性 别： 男 出生年月： 1982-07-21
职 称： 副教授 工作单位：
招生学科1： 发育生物学 招生学科2：
办公电话： E-mail： kqr721726@163.com

 开设研究生课程情况
课程编号 课程名称 课程级别 学时
209310 细胞重编程原理 硕士课 32
 近五年主持科研项目概况
(1) 转基因生物新品种培育科技重大专项——动物重要基因克隆与功能验证 子课题主持人；
(2) 国家自然科学基金青年基金——猪早期胚胎发育关键印迹基因挖掘与验证 课题主持人；
(3) 国家自然科学基金面上项目——猪细胞重编程和早期胚胎发育关键母源内源性小干扰RNA的挖掘与功能研究 课题主持人；
(4) 东北农业大学博士启动基金——外源基因在猪ROSA26位点遗传和表达稳定性研究课题主持人。


 近五年项目获奖概况
1） 《东北民猪体细胞核移植及绿色荧光蛋白转基因猪研究》获2009年黑龙江省科学技术奖自然科学类一等奖，排名第七；
2）论文《Green flurescent protein (GFP)transgenic pig produced by somatic cell nuclear transfer》获2010年黑龙江省自然科学技术学术成果奖，排名第四；
3） 《转基因猪中外源基因拷贝数和整合位点研究》获2010年全国教育改革优秀论文大赛一等奖，排名第一；
4）《猪体细胞核移植胚胎早期发育相关基因的表观遗传模式重建机理》获2014年黑龙江省畜牧科技一等奖，排名第二。


 近五年代表性著作、论文
1. Qingran Kong, Tang Hai, Yanshuang Mu, Jing Ma, Tianqing Huang, Qi Zhou, Zhonghua Liu, et al. 2014. Rosa26 locus supports tissue-specific promoter driving transgene expression specifically in pig. PLoS One. 9(9): e107945.
2. Jingyu Li, Yanjun Huan, Bingteng Xie, Qingran Kong*, Zhonghua Liu*, et al. 2014. Identification and characterization of an oocyte factor required for sperm nucleus decondensation in pig. Reproduction 148: 1-10. (corresponding author)
3. ZHOU Yang, ZHU Jiang, JIAO Mingxia, WANG Jiaqiang, KONG Qingran. 2014. The cloning and imprinting analysis of Slc22a18 and Slc22a3 in swine. Acta Veterinaria et Zootechnica Sinica. 45(10): 1600-1607. (corresponding author)
4. Qingran Kong, Bingteng Xie, Jingyu Li, Yanjun Huan, et al. 2014. Identification and characterization of an oocyte factor required for porcine nuclear reprogramming. JBC. 289(10):6960-8.
5. Qingran Kong, Guangzhen Ji, Bingteng Xie, Jingyu Li, et al. 2014. Telomere elongation facilitated by Trichostatin A in cloned embryos and pigs by somatic cell nuclear transfer. Stem cell review and report. 10(3):399-407.
6. Wenteng He*, Qingran Kong*, Yongqian Shi, Bingteng Xie, et al. 2013. Generation and Developmental Characteristics of Porcine Tetraploid Embryos and Tetraploid/diploid Chimeric Embryos. Genomics, Proteomics & Bioinformatics. 11(5): 327-33. (*equal contribution)
7. ZHOU Yang, ZHU Jiang, JIAO Mingxia, WANG Jiaqiang, KONG Qingran. 2013. Cloning and Imprinting Analysis of Asb4 and Grb10 in swine. China Animal Husbandry & Veterinary Medicine. 40(8): 61-67. (corresponding author)
8. Z. Yin*, Q.R. Kong*, Z.P. Zhao, et al. 2012. Position effect variegation and epigenetic modification of a transgene in a pig model. Genetics and Molecular Research. 11: 355-369. (*equal contribution)
9. Kui HU*, Qingran KONG*, Zeping ZHAO, et al. 2012. Assessment of Reproduction and Growth Performance of offspring derived from somatic cell cloned pigs. Animal Science Journal. 83: 639-643. (*equal contribution)
10. Q. R. KONG, M. L. WU, L. ZHANG, et al. 2011. Transgene insertion affects transcription and epigenetic modification of flanking host sequence in transgenic pigs. Cellular and Molecular Biology. 57: 1505-1512.
11. Qingran Kong, Meiling Wu, Zhenkun Wang, et al. 2011. Effect of Trichostatin A and 5-Aza-2'-deoxycytidine on transgene reactivation and epigenetic modification in transgenic pig fibroblast cells. Molecular and Cellular Biochemistry. 355: 157-165.
12. Qingran Kong, Jiang Zhu, et al. 2011. TSA improve transgenic porcine cloned embryo development and transgene expression. Hereditas. 33(7): 749-756.
13. Qingran Kong, Zhonghua Liu. 2011. Inheritance and expression stability of transgene in transgenic animals. Hereditas. 33(5): 504-511.
14. Kong Q, Wu M, Huan Y, Zhang L, Liu H, et al. 2009. Transgene Expression Is Associated with Copy Number and Cytomegalovirus Promoter Methylation in Transgenic Pigs. PLoS ONE. 4(8): e6679.
15. KONG Qing-Ran, WU Mei-Ling, ZHU Jiang, et al. 2009. Transgene Copy Number and Integration Site Analysis in Transgenic Pig. PBB. 36(12): 1617-1625.
16. Qing-Ran KONG, Zhong-Hua LIU. 2009. INTEGRATION SITE ANALYSIS IN TRANSGENIC PIGS BY THERMAL ASYMMETRIC INTERLACED (TAIL)-PCR AND JUNCTION PCR. Reproduction, fertility and development. 22(1): 371.
17. LIU ZhongHua, SONG Jun, WANG ZhenKun, TIAN JiangTian, KONG QingRan, et al. 2008. Green fluorescent protein (GFP) transgenic pig produced by somatic cell nuclear transfer. Chinese Science Bulletin. 53 (5): 556-560.


 近五年授权的专利等情况
1） 发明专利 《一种提高猪体细胞核移植效率的方法》已授权 公开号：ZL7.0 排名第一
2）发明专利 《猪ROSA26启动子及其应用》已授权 公开号：ZL7.8 排名第一
3） 发明专利 《猪ROSA26特异性整合位点及其应用》已授权 公开号：ZL4.0 排名第一


 其他获奖及荣誉称号