牦牛κ-酪蛋白新遗传变异体及进化分析

崔艳华¹, 曲晓军², 董爱军¹, 马莺¹

1.哈尔滨工业大学食品科学与工程学院;2.黑龙江省科学院微生物研究所



摘要:

为明确牦牛乳中κ-酪蛋白中遗传多样性,以κ-酪蛋白基因(CSN3)的IV外显子为研究对象,采用PCR-RFLP方法对麦洼牦牛的CSN3进行分析,旨在从分子水平揭示κ-酪蛋白在牦牛乳中遗传多样性分布.并以CSN3的IV外显子序列建立牛属κ-酪蛋白的系统发育树,从而分析进化关系.结果表明,牦牛乳中κ-酪蛋白多数以纯合体形式存在,并发现了一个新的变异体GU441771.牛属CSN3的系统发育树分析表明,κ-酪蛋白在进化上存在一定的物种特异性.

关键词:  牦牛乳  κ-酪蛋白  遗传多样性  聚合酶链式反应-限制性片段长度多态性

DOI：10.11918/j.issn.0367-6234.2012.02.026

分类号:S879.1

基金项目:国家自然科学基金资助项目(30871953;31071571)



The new variant and phylogenetic analysis of the κ-Casein Gene(CSN3) in yak

CUI Yan-hua¹, QU Xiao-jun², DONG Ai-jun¹, MA Ying¹

1.School of Food Science and Engineering,Harbin Institute of Technology,150090 Harbin,China;2.Institute of Microbiology,Heilongjiang Science Academy,150010 Harbin,China)

Abstract:

A polymorphism of κ-casein gene CSN3 exon IV was identified in Chinese Maiwa Yak by PCR-restriction fragment length polymorphism protocol to reveal the distribution of diversity of κ-casein in Maiwa Yak at the DNA sequence level.The sequences corresponding to the coding region of κ-casein gene exon IV were used for phylogenetic analysis to establish the phylogenetic relationship of CSN3 variants in the Bos genus.Experimental results indicated that most yak milks were with κ-casein BB genotype.A new variant was identified in Maiwa yak breed by analysis and entered into GenBank with accession number GU441771.The phylogenetic analysis presented κ-casein evolution was consistent with species at a degree.

Key words:  yak milk  κ-Casein  polymorphism  polymerase chain reaction-restriction fragment length polymorphism