Use of Tetrazolium Salt INT for Estimation of Biological Activity of Activated Sludge Cultivated in SBR Process
Jian-Hui Wang1,2, Jun Yin1, Hai Lu1, Ying-Zi Lin2
(1. School of Municipal and Environmental Engineering, Harbin Institute of Technology, Harbin 150090, China; ]2. Key Laboratory of Songliao Aquatic Environment, Ministry of Education, Jilin Jianzhu University, Changchun 130118, China)
Abstract:
The tetrazolium salt 2-(4-Iodophenyl)-3-(4-nitrophenyl)-5-phenyltetrazolium chloride (INT) was used as a tool for estimating the activity of the electron transport system (ETS) in activated sludge in a 40 L sequencing batch reactor (SBR) and domestic sewage as the organic substrate. The activity of INT-ETS during one SBR cycle, and the effect of the ammonia concentration and the concentration of organic matter influent on the INT-ETS activity were investigated. The results show that: the use of INT is reliable in estimating of biological activity of activated sludge of SBR system; Biological activity of organic matter biodegradation, nitrification and denitrification process in SBR system reduce orderly. Obviously, INT-ETS activity reduces from 232.59 mg/(g·h) to 190.65 mg/(g·h) at first and then decreases to 113.88 mg/(g·h) when influent concentration of COD and NH4+-N is 300 mg/L and 40 mg/L respectively. In addition, various influent Nitrogen (NH4+-N are 14.5 mg/L and 42.0 mg/L) and organic shock loading (COD are 293 mg/L and 685 mg/L) experimentations cure prove that operational conditions have no obvious effect on INT-ETS variation rule. However, the time of the appearance of feature points marking different reaction phase is influenced.
Key words: ETS activity activated sludge SBR tetrazolium salt
DOI：10.11916/j.issn.1005-9113.2014.02.015
Clc Number:X703.1
Fund:
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Use of Tetrazolium Salt INT for Estimation of Biological Activity of Activated Sludge Cultivated in
本站小编 哈尔滨工业大学/2019-10-23
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