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Genomic and proteomic analysis of soybean heritable variations induced by space flight

本站小编 哈尔滨工业大学/2019-10-23

Genomic and proteomic analysis of soybean heritable variations induced by space flight

HeJie, GaoYong, SUN Ye-qing

Dept.of Life Science and Engineering,Harbin Institute of Technology,Harbin 150001,China



Abstract:

To analyze the biological effects of space environment,the diversity of genomic DNA between the space flight soybean 194(4126) with phenotype of good yield and good fruit quality induced by space flight and the soybean with ground control was studied by amplified fragment length polymorphism(AFLP) method,and the polymorphism of space flight soybean 194(4126) was 3.56%.The differences of protein expression of seeds and leaves between the two kinds of soybeans were analysed by two-dimensional electrophoresis,PDQuest software and MALDI-TOF mass spectrometry.Results show that the loss and decrease of protein expression in 194(4126) soybean are subjected to the space fight of seeds,and three special proteins including Dehydrin,MAT1 and ceQORH are identified.It is concluded that the space environment changes the phenotype and genotype of soybeans due to the space flight of seeds.

Key words:  space environment  genomic  proteomic  soybean

DOI:10.11916/j.issn.1005-9113.2009.04.026

Clc Number:S565.1

Fund:


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