基本信息科学研究教育教学论文专著
基本信息

顾宁，男，教授，博士生导师；哈尔滨工业大学生命科学与技术学院。

研究领域
顾宁于2010年1月，被哈尔滨工业大学生命与工程系以海外人才的形式引进，并获得国家自然科学基金及哈尔滨工业大学科研启动基金的资助。现在，主要从事有关药理学与内分泌疾病领域的研究.

现主要研究方向：

1. 糖尿病、肥胖症等内分泌疾病相关基因功能的研究

2. 纳米粒子、小分子化合物的药理及毒理研究

3. 环境污染物对健康影响及分子机制研究

现负责科研项目有：

1.【纳米材料对不同发育时期小鼠血糖代谢的影响及其机制的研究】

国家自然科学基金 2017-2020负责人

2.【特定的转录因子重组蛋白质诱导成纤维细胞直接重编程为软骨细胞及其安全性的研究】

国家自然基金 2012-2016 负责人

3.【水环境中TiO2纳米颗粒对血糖代谢的影响极其机制的研究】

城市水资源与水环境国家重点实验室 2015-2016负责人

4.【转录因子重组蛋白改善间充质干细胞增殖能力的研究】

黑龙江省归国留学基金 2012-2015 负责人5.【水环境中的纳米粒子对内分泌系统的毒理效应及其机制的研究】

城市水资源与水环境国家重点实验室2011-2014负责人
6.【关于改善重组蛋白诱导iPS细胞效率的研究】
国家自然科学基金 2011-2011 负责人

工作经历
时间工作经历
2016.1-

2010.1-2015.12

哈尔滨工业大学 生命科学与技术学院 教授

哈尔滨工业大学 生命科学与技术学院 副教授
2009.4-2010.1日本 产业技术综合研究所 研究员
2007.4-2009.3日本 京都大学 学术振兴会（JSPS）研究员


教育经历
2004.4-2007.3日本　京都大学　　　 博士2002.4-2004.3日本　京都大学　　　 硕士1998.4-2002.3日本　京都教育大学　 学士


科研成果
发表论文：

1)Qian Guo, Hailong Hu, Xiaohuan Liu, DaQian Yang, Yao Yin, Boya Zhang, Hongjuan He, Yuri Oh, Qiong Wu, Chuanpeng Liu, Ning Gu, C/EBPβ mediates palmitate-induced musclin expression via the regulation of PERK/ATF4 pathways in myotubes. Am J Physiol Endocrinol Metab. 2019 Apr 9. doi: 10.1152/ajpendo.00478.2018. [Epub ahead of print]

2）Qian Guo，Hailong Hu，Ying Zhoua，Yuheng Yan，Xiangjuan Wei，Xingpei Fan，Daqian Yang，Hongjuan He，Yuri Oh，Kun Chen，Qiong Wu，Chuanpeng Liua，Ning Gu，Glucosamine induces increased musclin gene expression through endoplasmic reticulum stress-induced unfolding protein response signaling pathways in mouse skeletal muscle cells. Food Chem Toxicol, 2019 March; 125: 95-105

3）Hu Hailong, Li Li, Guo Qian, Zong He, Yan Yuheng, Yin Yao, Wang Yu, Oh, Yuri, Feng Yujie, Wu Qiong, Ning Gu,RNA sequencing analysis shows that titaniumdioxide nanoparticles induce endoplasmic reticulum stress, which has a central role in mediating plasma glucose in mice. Nanotoxicology, 2018 May;12(4):341-356.

4) Guo Qian, Jin Sanli, Hu Hailong, Zhou Ying, Yan Yuheng, Zong He, Wang Yu, He Hongjuan, Oh Yuli, Liu Chuanpeng, Ning Gu, Hypoxia in 3T3-L1 adipocytes suppresses adiponectin expression via the PERK and IRE1 unfolded protein response. Biochem Biophys Res Commun. 2017 Nov 4;493(1):346-351

5) Hu Hailong, Li Li, Guo Qian, Jin Sanli, Zhou Ying, Oh Yuri, Feng Yujie, Wu Qiong, Ning Gu, A mechanistic study to increase understanding of titanium dioxide nanoparticles-increased plasma glucose in mice. Food Chem Toxicol, 2016, 95:175-187.

6) Gu Ning, Hu Hailong, Guo Qian, Jin Sanlin, Wang Changlin, Oh Yuri, Feng Yujie, Wu Qiong, Effects of oral administration of titanium dioxide fine-sized particles on plasma glucose in mice. Food Chem Toxicol, 2015, 86:124-131.

7) Gu Ning, Guo Qian, Mao Ke, Hu Hailong, Jin Sanlin, Zhou Ying, He Hongjuan, Oh Yuri, Liu Chuanpeng, Wu Qiong, Plasmitate increases musclin gene expression through activation of PERK signaling pathway in C2C12 myotubes. Biochem Biophys Res Commun, 2015, 467: 521-526.

8) Hu Hailong, Guo Qian, Wang Changlin, Ma Xiao, He Hongjuan, Oh Yuri, Feng Yujie, Wu Qiong, Gu Ning, Titanium dioxide nanoparticles increase plasma glucose via reactive oxygen species-induced insulin resistance in mice. J. App. Toxicol. 2015.

9) Hu Hailong, Li Li, Wang Changlin, He Hongjuan, Mao Ke, Ma Xiao, Shi Ruoyu, Oh Yuri, Zhang Fengwei, Lu Ying, Wu Qiong, Gu Ning, 4-Phenylbutyric acid increases GLUT4 gene expression through suppression of HDAC5 but not endoplasmic reticulum stress. Cell Physiol Biochem. 2014;33(6):1899-910.

10) Wang CL, Diao YX, Xiang Q, Ren YK, Gu Ning, Diabetes attenuates the inhibitory effects of endomorphin-2, but not endomorphin-1 on gastrointestinal transit in mice. Eur J Pharmacol. 2014 Sep 5;738:1-7.

11) Wang CL, Xiang Q, Diao YX, Ren YK, Gu Ning, Differential antinociceptive effects of intrathecal administration of C-terminal esterified endomorphin-2 analogues in mice. Eur J Pain. 2014 Sep;18(8):1157-64.

12) Wang CL, Ren YK, Xiang Q, Wang Y, Gu Ning, Lu C, Wang R, Characterization of opioid activities of endomorphin analogs with C-terminal amide to hydrazide conversion. Neuropeptides. 2013 Oct;47(5):297-304.

13) Gu Tiantian, He Hongjuan, Xing Yanjiang, Liu Qi, Gu Ning, Sugimoto Kenkichi, Jiang Huijie and Wu Qiong. Expression of non-coding RNA AB063319 derived from Rian gene during mouse development. J Mol Histol. 2011 Feb 9[Epub ahead of print]

14) Ikegami Dasuke, Iwai T, Ryo Senyi, Gu Ning, Sugiyama Tasuke, Oh Isamu, Yoshikawa Hiroyuki, Tsumaki Nobuyuki. Identification of small molecular compounds and fabrication of its aqueous solution by laser-ablation, expanding primordial cartilage. Osteoarthritis Cartilage. 19(2), 233-41 2011.

15) Gu Ning, Nagatomo Fumiko, Fujino Hidemi, Takeda Isao, Tsuda Kinsuke, Ishihara Akihiko. Effects of hyperbaric oxygenation on oxidative capacity in the soleus and plantaris muscles of type 2 diabetic rats. Diabetes Technol. Ther. 12(2):125-133, Feb, 2010.

16) Gu Ning, Tsuda Mariko, Matsunaga Tetsuro, Adachi Tetsuya, Yasuda Koichiro, Ishihara Akihiko, Tsuda Kinsuke. Glucose regulation of DPP-IV gene expression is mediated by HNF-1alpha in epithelial intestinal cells. Clin Exp Pharm Phys. 35(12), 1433-1439, May, 2008.

17) Gu Ning, Adachi Tetsuya, Matsunaga Tetsuro, Tsujimoto Gozoh, Ishihara Akihiko, Yasuda Koichiro, Tsuda Kinsuke. HNF-1alpha participates in glucose regulation of sucrase-isomaltase (SI) gene expressions in small intestinal epithelial cells. Biochem Biophys Res Commun. 353(3), 617-622, Feb, 2007.

18) Gu Ning, Adachi Tetsuya, Matsunaga Tetsuro, Takeda Jun, Tsujimoto Gozoh, Ishihara Akihiko, Yasuda Koichiro, Tsuda Kinsuke. Mutant HNF-1alpha and mutant HNF-1beta identified in MODY3 and MODY5 downregulate DPP-IV gene expression in Caco-2 cells. Biochem Biophys Res Commun. 346(3), 1016-1023, Aug, 2006.

19) Gu Ning, Adachi Tetsuya, Takeda Jun, Aoki Norihiko, Tsujimoto Gozoh, Ishihara Akihiko, Tsuda Kinsuke, Yasuda Koichiro. Sucrase-isomaltase gene expression is inhibited by mutant hepatocyte nuclear factor (HNF)-1alpha and mutant HNF-1beta in Caco-2 cells. J Nutrl Sci Vitaminol. 52(2), 105-112, Apr, 2006.

19) Gu Ning, Suzuki Naoko, Takeda Jun, Adachi Tetsuya, Tsujimoto Gozoh, Ishihara Akihiko,Tsuda Kinsuke, Yasuda Koichiro. Effect of mutations in HNF-1alpha and HNF-1beta on the transcriptional regulation of human sucrase-isomaltase (SI) in Caco-2 cells. Biochem Biophys Res Commun. 325(1), 308-313, Oct, 2004.


授权专利
1) 顾宁, 胡海龙, 毛轲, 郭倩. 一种促进肌肉细胞中GLUT4基因表达的方法: ZL2**.1. 2016-09-07.

2) 顾宁, 岳磊，胡海龙, 郭倩, 毛轲. SB505124在制备治疗软骨损伤的药物中的应用:ZL2**.8. 2016-01-27.

3) 顾宁, 岳磊, 胡海龙. 一种提高人间充质干细胞分化能力的方法:ZL2**.3. 2016-08-24.

4) 顾宁, 马卓, 胡海龙, 郭倩. 一种新的检测组织中纳米颗粒的方法: ZL2**.7. 2017-01-04.

科研项目

项目名称【特定的转录因子重组蛋白质诱导成纤维细胞直接重编程为软骨细胞及其安全性的研究】

项目来源国家自然基金

开始时间2012-01-01

结束时间2016-01-01

担任角色负责

项目类别横向项目

项目状态完成



项目名称【转录因子重组蛋白改善间充质干细胞增殖能力的研究】

项目来源黑龙江省归国留学基金

开始时间2012-01-01

结束时间2015-01-01

担任角色负责

项目类别横向项目

项目状态完成



项目名称【水环境中的纳米粒子对内分泌系统的毒理效应及其机制的研究】

项目来源城市水资源与水环境国家重点实验室

开始时间2011-01-01

结束时间2014-01-01

担任角色负责

项目类别横向项目

项目状态完成



项目名称【关于改善重组蛋白诱导iPS细胞效率的研究】

项目来源国家自然科学基金

开始时间2011-01-01

结束时间2011-01-01

担任角色负责

项目类别横向项目

项目状态完成



项目名称纳米材料对不同发育时期小鼠血糖代谢的影响及其机制的研究

项目来源国家自然科学基金

开始时间2017

结束时间2020

担任角色负责

项目类别纵向项目

项目状态进行中



项目名称水环境中 TiO2纳米颗粒对血糖代谢的影响极其机制的研究

项目来源城市水资源与水环境国家重点实验室

开始时间2015.01.01

结束时间2016.12.30

担任角色负责

项目类别纵向项目

项目状态进行中



研究领域
干细胞的增殖与分化机制、体细胞重编程等研究

糖尿病，肥胖症等内分泌疾病的发病机制和治疗方法的研究

纳米粒子及小分子化合物的药理研究

团队成员
胡海龙：博士, 研究方向: 纳米毒理与内分泌系统疾病.
郭 倩：博士, 研究方向: UPR信号通路参与棕榈酸诱导的musclin基因表达的初步研究.

周 莹：硕士, 研究方向: 葡萄糖胺调控Musclin、CTRP15及FNDC5基因表达及机制的初步研究

闫玉恒：硕士, 研究方向: 糖尿病机制研究.

宗 贺：硕士, 研究方向: 糖尿病机制研究.

讲授课程
《生物制品》

研究生学位课，了解生物制品的制备方法和基本要求，掌握生物制品制备的基本原理及制备技术，了解生物制品领域的最新进展及发展趋势。

《生命科学基础与应用》
校管核心课课，旨在扩大学生的知识面，培养学生生命科学的思维方式，将生命科学的思想、方法运用到他们所从事的工作领域。

《纳米技术与基因研究》
新生研讨课，以纳米技术在基因领域的研究为重点，讲授纳米技术在基因靶向医疗、基因检测诊断等领域的最新研究进展，与同学们共同分析存在的科学问题及解决方法，探讨纳米技术在生命医学领域的未来发展方向与前景。



招生信息
招收硕士：1-2名
招收博士：1名

论文期刊

论文标题Effects of hyperbaric oxygenation on oxidative capacity in the soleus and plantaris muscles of type 2 diabetic rats.

作者Gu Ning, Nagatomo Fumiko, Fujino Hidemi, Takeda Isao, Tsuda Kinsuke, Ishihara Akihiko.

期刊名称Diabetes Technol

期卷12(2):125-



论文标题 Glucose regulation of DPP-IV gene expression is mediated by HNF-1alpha in epithelial intestinal cells.

作者Gu Ning, Tsuda Mariko, Matsunaga Tetsuro, Adachi Tetsuya, Yasuda Koichiro, Ishihara Akihiko, Tsuda Kinsuke.

期刊名称Clin Exp Pharm Phys

期卷35(12), 14



论文标题HNF-1alpha participates in glucose regulation of sucrase-isomaltase (SI) gene expressions in small intestinal epithelial cells.

作者Gu Ning, Adachi Tetsuya, Matsunaga Tetsuro, Tsujimoto Gozoh, Ishihara Akihiko, Yasuda Koichiro, Tsuda Kinsuke.

期刊名称Biochem Biophys Res Commun.

期卷353(3), 61



论文标题Mutant HNF-1alpha and mutant HNF-1beta identified in MODY3 and MODY5 downregulate DPP-IV gene expression in Caco-2 cells.

作者Gu Ning, Adachi Tetsuya, Matsunaga Tetsuro, Takeda Jun, Tsujimoto Gozoh, Ishihara Akihiko, Yasuda Koichiro, Tsuda Kinsuke.

期刊名称Biochem Biophys Res Commun.

期卷346(3), 10



论文标题Sucrase-isomaltase gene expression is inhibited by mutant hepatocyte nuclear factor (HNF)-1alpha and mutant HNF-1beta in Caco-2

作者Gu Ning, Adachi Tetsuya, Takeda Jun, Aoki Norihiko, Tsujimoto Gozoh, Ishihara Akihiko, Tsuda Kinsuke, Yasuda Koichiro.

期刊名称J Nutrl Sci Vitaminol

期卷52(2), 105



论文标题Effect of mutations in HNF-1alpha and HNF-1beta on the transcriptional regulation of human sucrase-isomaltase (SI) in Caco-2 cel

作者 Gu Ning, Suzuki Naoko, Takeda Jun, Adachi Tetsuya, Tsujimoto Gozoh, Ishihara Akihiko,Tsuda Kinsuke, Yasuda Koichiro.

期刊名称Biochem Biophys Res Commun

期卷325(1), 30



论文标题4-PBA Increases GLUT4 Gene Expression through Suppression of HDAC5

作者Hailong Hu, Li Li, Changlin Wang, Hongjuan He, Ke Mao, Xiao Ma, Ruoyu Shi, Yuri Oh, FengWei Zhang, Ying Lu, Qiong Wu, Ning Gu

期刊名称Cell Physiol Biochem

期卷2014;33:18



论文标题Diabetes attenuates the inhibitory effects of endomorphin-2, but not endomorphin-1 on gastrointestinal transit in mice.

作者Wang CL, Diao YX, Xiang Q, Ren YK, Gu N.

期刊名称Eur J Pharmacol

期卷2014 Sep 5



论文标题Differential antinociceptive effects of intrathecal administration of C-terminal esterified endomorphin-2 analogues in mice.

作者Wang CL, Xiang Q, Diao YX, Ren YK, Gu N.

期刊名称Eur J Pain.

期卷2014 Sep;1



论文标题Characterization of opioid activities of endomorphin analogs with C-terminal amide to hydrazide conversion.

作者Wang CL, Ren YK, Xiang Q, Wang Y, Gu N, Lu C, Wang R.

期刊名称Neuropeptides

期卷2013 Oct;4



论文标题 Expression of non-coding RNA AB063319 derived from Rian gene during mouse development.

作者Gu Tiantian, He Hongjuan, Xing Yanjiang, Liu Qi, Gu Ning, Sugimoto Kenkichi, Jiang Huijie and Wu Qiong.

期刊名称 J Mol Histol.



论文标题Identification of small molecular compounds and fabrication of its aqueous solution by laser-ablation, expanding primordial cart

作者Ikegami Dasuke, Iwai T, Ryo Senyi, Gu Ning, Sugiyama Tasuke, Oh Isamu, Yoshikawa Hiroyuki, Tsumaki Nobuyuki.

期刊名称Osteoarthritis Cartilage.

期卷19(2), 233



论文标题Characteristics of skeletal muscle in rats with diabetes, hypertension, obesity,and hyperlipidemia.

作者Nagatomo Fumiko, Gu Ning, Fujino Hidemi, Takeda Isao, Tsuda Kinsuke, Ishihara Akihiko.

期刊名称J Atheroscler Thromb

期卷16(5), 576



论文标题 Effects of hyperbaric exposure with high oxygen concentration on reactive oxygen metabolites in type II collagen-induced arthri

作者Nagatomo Fumiko, Gu Ning, Fujino Hidemi, Okiur Tomonori, Morimatsu Fumiki, Takeda Isao, Ishihara Akihiko.

期刊名称 Free Radical Research



论文标题Association of UCP2 and UCP3 polymorphisms with autonomic nervous system functionin young Japanese males.

作者Matsunaga Tetsuro, Gu Ning, Yamazaki Hanae, Tsuda Mariko, Adachi Tetsuya, Yasuda ,Koichiro, Moritani, Toshio, Tsuda Kinsuke, Non

期刊名称J Hypertens.

期卷 27(2), 30



论文标题 Bone density of the femur and fFiber cross-sectional area and oxidative enzyme activity of the tibialis anterior muscle in type

作者Okiura Tomonori, Nagatomo Fumiko, Gu Ning, Taguchi Yasuki, Morimatsu Fumiki and Ishihara Akihiko.

期刊名称J Physiol Sci.

期卷58(4), 221



论文标题ZFAT expression in B and T lymphocytes andidentification of ZFAT-regulated genes.

作者Koyanagi Midori, Nakabayashi Kazuhiko, Fujimoto Takahiro, Gu Ning, Baba Iwai, Sasazuki Takehiko and Shirasawa Senji.

期刊名称Genomics.

期卷91(5), 451



论文标题Effects of hyperbaric exposure with high oxygenconcentration on glucose and insulin levels and skeletal muscle fiber properties

作者Yasuda Koichiro, Adachi Tetsuya, Gu Ning, Matsumoto Akiko, Matsunaga Tetsuro, Tsujimoto Gozoh, Ishihara Akihiko.

期刊名称Muscle Nerve

期卷35(3), 337



论文标题 Association of beta-adrenoceptor polymorphisms with cardiac autonomic modulation in Japanese males.

作者Matsunaga Tetsuro, Yasuda Koichiro, Adachi Tetsuya, Gu Ning, Yamamura Tsubasa, Moritani Toshio, Tsujimoto Gozoh, Tsuda Kinsuke.

期刊名称Am Heart J.

期卷154(4), 75



论文标题Fiber type distribution, succinate dehydrogenese and peroxisome proliferator- activatedreceptor gamma coactivator- 1alpha gene e

作者 Adachi Tetsuya, Kikuchi Naoko, Yasuda Koichiro, Anahara Reko, Gu Ning, Matsunaga Tetsuro, Yamamura Tsubasa, Tsujimoto Gozou, Ts

期刊名称Exp Physiol.

期卷92(2), 449



论文标题 Hyperbaric exposure with high oxygen concentration inhibits growth-associated increase in the glucose level of diabetic Goto-Ka

作者Yasuda Koichiro, Adachi Tetsuya, Gu Ning, Tsuda Kinsuke, Kikuchi Naoko, Matsunaga Tetsuro, Ishihara Akihiko.

期刊名称Diabetes Obes Metab.

期卷8(6), 714-



论文标题Alpha-adrenoceptor gene variants and autonomic nervous system function in a young healthy Japanese population.

作者Matsunaga Tetsuro, Yasuda Koichiro, Adachi Tetsuya, Gu Ning, Yamamura Tsubasa, Moritani Toshio, Tsujimoto Gozoh, Tsuda Kinsuke.

期刊名称J Hum Genet

期卷52(1), 28-



论文标题Association of C825T Polymorphism of G protein beta3 subunit with the autonomic nervous system in young healthy japanese individ

作者Matsunaga Tetsuro, Nagasumi Kae, Gu Ning, Nishikino Mariko, Ueda Yukihiko, Moritani Toshio, Aoki Norihiko, Tsuda Kinsuke, Yasuda

期刊名称Am J Hypertens.

期卷18(4), 523



论文标题T393C polymorphism of GNAS1 associated with the autonomic nervous system in young, healthy Japanese subjects.

作者 Yasuda Koichiro, Matsunaga Tetsuro, Moritani Toshio, Nishikino Mariko, Gu Ning, Yoshinaga Mariko, Nagasumi Kae, Yamamura Tsubas

期刊名称Clin Exp Pharm Phys

期卷31(9), 597



论文标题Titanium dioxide nanoparticles increase plasma glucose via reactive oxygen species-induced insulin resistance in mice

作者Hu Hailong, Guo Qian, Wang Changlin, Ma Xiao, He Hongjuan, Oh Yuri, Feng Yujie, Wu Qiong, Gu Ning,

期刊名称J. App. Toxicol.



论文标题 Effects of oral administration of titanium dioxide fine-sized particles on plasma glucose in mice

作者Gu N, Hu H, Guo Q, Jin S, Wang C, Oh Y, Feng Y, Wu Q

期刊名称Food Chem Toxicol

期卷86:124-31



论文标题Palmitate increases musclin gene expression through activation of PERK signaling pathway in C2C12 myotubes

作者Gu N, Guo Q, Mao K, Hu H, Jin S, Zhou Y, He H, Oh Y, Liu C, Wu Q

期刊名称Biochem Biophys Res Commun

期卷467(3):521-6



论文标题A mechanistic study to increase understanding of titanium dioxide nanoparticles-increased plasma glucose in mice

作者Hailong Hu, Li Li, Qian Guo, Sanli Jin, Ying Zhou, Yuri Oh, Yujie Feng, Qiong Wu, Ning Gu*

期刊名称Food Chem Toxicol

期卷95:175-187



专利
1) 顾宁, 胡海龙, 毛轲, 郭倩. 一种促进肌肉细胞中GLUT4基因表达的方法: 中国, CN**A. 2014-05-28.

2) 顾宁, 胡海龙, 郭倩, 毛轲. SB505124在制备治疗软骨损伤的药物中的应用: 中国, CN**A. 2014-08-06.

3) 顾宁, 岳磊, 胡海龙. 一种提高人间充质干细胞分化能力的方法: 中国, CN**A. 2014-08-27.

4) 顾宁, 马卓, 胡海龙, 郭倩. 一种新的检测组织中纳米颗粒的方法: 中国, CN**A. 2014-12-10.