摘要:为探索黄瓜与西芹间作后对黄瓜土壤真菌多样性变化规律的影响，本试验以黄瓜与西芹间作种植模式为处理，黄瓜单作和西芹单作种植模式为对照，进行不同处理土壤ITS真菌群落多样性高通量测序分析。结果表明：黄瓜与西芹间作土壤真菌Alpha多样性指数降低，Observed species指数、Shannon指数、Chao1指数和Simpson指数与对照相比均达到最低值，但差异均不显著（P>0.05）。在门分类水平上，共检测到5个菌门，其中，子囊菌门（Ascomycota）、接合菌门（Zygomycota）和担子菌门（Basidiomycota）为3个主要菌门；黄瓜与西芹间作处理前3个菌门所占比例最高，达95.50%。在属分类水上，共检测到329属，相对丰度比例为前12的菌属占所有检测出菌属的49.4%；黄瓜与西芹间作处理检测的菌属所占比例最高，达50.72%，其次为黄瓜单作和西芹单作，分别为50.47%和47.17%。因此，黄瓜与西芹间作处理改变了黄瓜土壤真菌群落的结构和组成，丰富了黄瓜土壤真菌的群落多样性，为以后设施黄瓜连作土壤微生态环境改善和修复提供科学依据。
关键词:黄瓜与西芹间作/
ITS测序/
土壤真菌/
真菌群落/
多样性指数
Abstract:Cucumber wilt is a soil-borne disease that retards cucumber production. Intercropping can be used to reduce the prevalence of plant soil-borne diseases, and celery is widely used in intercropping because of its allelopathic property. This study was carried out to explore the effect of cucumber and celery intercropping on cucumber fusarium wilt and soil fungal diversity. Soils from celery monoculture, cucumber monoculture, and cucumber and celery intercropping were analyzed for fungal ITS diversity. The amount of OUT and the Alpha diversity of soil fungi were reduced with the intercropping. Observed species index, Shannon index, Chao1 index, and Simpson index showed reducing for the intercropping though change was not significant. Five fungal phyla were identified, among which Ascomycota, Zygomycota, and Basidiomycota were the primary ones, accounting for up to 95.50% of the total phyla with the intercropping. Cucumber and celery intercropping and cucumber monoculture appeared to be in the same cluster of phyla, clearly distinct from celery monoculture. There were 329 fungal genera that were identified, and half of their abundance was accounted for by 12 genera, specifically 50.72%, 50.47%, and 47.17% for cucumber and celery intercropping, cucumber monoculture, and celery monoculture, respectively. The genus cluster distribution was consistent with the phylum distribution. Cucumber and celery intercropping altered the structure and composition of the soil fungal community, specifically enriching fungal diversity. The information provided an insight into the use of biological means to control soil-borne diseases encountered in cucumber production.
Key words:Cucumber and celery intercropping/
ITS fungal sequencingtechnique/
Soil fungi/
Fungal community/
Diversity indexes
PDF全文下载地址:
http://www.ecoagri.ac.cn/article/exportPdf?id=a8173f97-1cc3-466e-8d6c-1eb73e2f66c8
