论文题目: AUXIN RESPONSE FACTOR3 Regulates Floral Meristem Determinacy By Repressing Cytokinin Biosynthesis and Signaling

论文编号:
作者: Ke Zhang（张科）, Ruozhong Wang（王若仲）, Hailing Zi（訾海玲）, Yongpeng Li（李永鹏）, Xiuwei Cao（曹秀卫）, Dongming Li（李东明）, Lin Guo（郭琳）, Jianhua Tong（童建华）, Yanyun Pan（潘延云）, Yuling Jiao（焦雨铃）, Renyi Liu（刘仁义）, Langtao Xiao（肖浪涛） and Xigang Liu（刘西岗）
刊物名称: Plant Cell
卷期页码: http://doi.org/10.1105/tpc.17.00705, ,
发表年度: 2018
DOI: http://doi.org/10.1105/tpc.17.00705
摘要: Successful floral meristem (FM) determinacy is critical for subsequent reproductive development and the plant life cycle. Although the phytohormones cytokinin and auxin interact to coregulate many aspects of plant development, whether and how cytokinin and auxin function in FM determinacy remain unclear. Here we show that in Arabidopsis thaliana, cytokinin homeostasis is critical for FM determinacy. In this developmental context, auxin promotes the expression of AUXIN RESPONSE FACTOR3 (ARF3) to repress cytokinin activity. ARF3 directly represses the expression of ISOPENTENYLTRANSFERASE (IPT) family genes and indirectly represses LONELY GUY (LOG) family genes, both of which encode enzymes required for cytokinin biosynthesis. ARF3 also directly inhibits the expression of ARABIDOPSIS HISTIDINE KINASE4 (AHK4), a cytokinin receptor gene, resulting in reduced cytokinin activity. Consequently, ARF3 controls cell division by regulating cell cycle gene expression through cytokinin. In flowers, we show that AGAMOUS (AG) dynamically regulates the expression of ARF3 and IPTs, resulting in coordinated regulation of FM maintenance and termination through cell division. Moreover, genome-wide transcriptional profiling revealed both repressive and active roles for ARF3 in early flower development. Our findings establish a molecular link between AG and auxin/cytokinin and shed light on the mechanisms of stem cell maintenance and termination in the FM.