论文题目: miR172b Controls the Transition to Autotrophic Development Inhibited by ABA in Arabidopsis

论文编号:
作者: Zou, YM (邹艳敏); Wang, YN (王幼宁); Wang, LX (Wang, Lixiang); Yang, L (Yang, Lei); Wang, R (Wang, Rui); Li, X (李霞)
刊物名称: PLoS ONE
卷期页码: 卷: 8, 期: 5, 文献号: e64770
发表年度: 2013
DOI: http://dx.doi.org/10.1371/journal.pone.0064770
摘要: Seedling establishment is a critical phase in the life of plants when they are the most vulnerable to environment. Growth arrest at post-germinative stage under stress is the major adaptive strategy to help germinating seedlings to survive a spectrum of stressful conditions. ABA signaling is the key pathway to control stress-induced developmental arrest. However, mechanisms controlling the phase transition under abiotic stress are not fully understood. Here, we described miR172b as a new key regulator controlling transition of germinating seedlings from heterotrophic to autotrophic growth under osmotic stress inArabidopsis. We showed that miR172b and its targetSNZwere co-expressed during early seedling development. Expression of miR172b andSNZwas low after radicle emergence and sharply increased at the checkpoint to autotrophic development under normal conditions. Interestingly, activation of miR172b andSNZwas completely abolished by ABA and osmotic stress. miR172b overexpression andsnz-1exhibited increased sensitivity to ABA and osmotic stress during specific post-germinative stage, and resulted in higher expression ofABI3,ABI5and downstream genes, such asEm6andRAB18, than wild type under ABA treatment. Our results revealed that miR172b is a critical regulator specifically controlling cotyledon greening during post-germinative growth by directly targetingSNZunder ABA treatment and osmotic stress.