论文题目: Relationship between soil properties and the bias of N2O reduction by acetylene inhibition technique for analyzing soil denitrification potential

论文编号:
作者: Shuping Qin (秦树平)， Haijing Yuan（袁海静），Wenxu Dong（董文旭），Chunsheng Hu（胡春胜，通讯作者），Oene Oenema，Yuming Zhang（张玉铭）
刊物名称: Soil Biology & Biochemistry
卷期页码: , In press,
发表年度: 2013
DOI: http://dx.doi.org/10.1016/j.soilbio.2013.07.016
摘要: The acetylene inhibition technique (AIT) has been widely used to measure soil denitrification potential (SDP), but has been criticized also for underestimating the actual SDP due to limitations of the AIT. Possible effects of soil properties on the bias of the AIT-derived SDP have not been thoroughly investigated yet. The study presented here therefore aimed at quantifying the relationships between soil texture and nutrient contents and the bias of AIT-derived SDP. A total of 26 soils with a wide range of clay and nutrient contents were incubated according to the standard procedure of AIT for assaying SDP. Incubation flasks were made anaerobic by gas substitution with pure helium (99.999%). Changes in the N₂and N₂O concentrations in the He headspace were measured using gas chromatography. The emission rates of N₂O and N₂were calculated to quantify the bias of the AIT-derived SDP (expressed as the percentage of the actual SDP that was not accounted for by AIT). The results showed that the bias ranged from 8 to 98%. The bias was negatively correlated (P<0.05) with the clay, silt, organic matter and nutrient contents of the soils. These results indicate that the bias of the AIT-derived SDP was higher in low-fertility soils than in fertile soils. We recommend that soil properties are taken into account when interpreting results of AIT-derived SDP values.