职称职务：教授、博士生导师
研究方向：（1）蛋白质-蛋白质作用的分子机制；
　　　　　(2)生物分子信息与工程；(3)多肽药物筛选与设计
实验室位置：生科院5112室
电话：**
Email:ylwu@whu.edu.cn



学习简历
　1999.9-2002.7武汉大学化学系博士研究生
　1996.9-1999.7南昌大学化学系硕士研究生
　1992.9-1996.7南昌大学化学系本科

工作简历
　2009.11-至今　　　武汉大学生命科学学院，教授、博士生导师
　2008.9-2009.10　　武汉大学生命科学学院，副教授、博士生导师
　2007.10-2008.9　　加拿大Ambrilia生物制药公司，多肽药物设计咨询专家
　2004.11-2008.8　　武汉大学生命科学学院副教授、硕士生导师
　2002.9-2004.10　　武汉大学生命科学学院博士后

主要学术兼职
　　2013.11-至今中国毒理学会生物毒素毒理专业委员会副主任委员
　　2009.11-2013.10中国毒理学会生物毒素毒理专业委员会秘书长

主要研究领域和兴趣
　　主要领域为蛋白质相互作用的分子机制、多肽分子工程及多肽药物发现研究。
　　主要研究兴趣为作用细胞膜离子通道药用蝎活性多肽的基础与应用基础研究。毒蝎在地球上生存进化了4亿多年，在我国具有2000多年的药用史。蝎的主要药用成份为蝎尾毒液中数量众多且结构功能多样的蝎活性多肽（又称为“蝎毒素”或“蝎毒素多肽”），这些多肽通过选择性地作用动物和人细胞膜不同种类膜离子通道蛋白及其它受体蛋白，从而具有抗病毒、抗菌、抗类风湿性关节炎、治疗多发性硬化症等药理功能。通过系统深入研究蝎活性肽的结构功能关系及其与靶标蛋白质的作用机制，建立了蝎活性肽与靶标蛋白相互作用的“人工控制识别”新分子工程技术，筛选设计与鉴定具有抗病毒、抗耐药菌、抗类风湿性关节炎功能等重要药用前景的蝎活性肽，推进我国特有蝎活性多肽的生物医药应用。

教学情况
　　生物信息学，36学时，授课对象为本科生和研究生

承担科研项目
　　先后承担了武汉市科技项目、湖北省杰出青年基金项目、教育部新世纪人才基金项目、国家自然科学基金项目、国家重大多肽新药研发课题、国家科技部973药物筛选课题等。

博士后招聘
　　本实验室常年招聘博士后，热烈欢迎不同学科背景的博士到本实验室从事科研合作研究。

代表性研究论文（*为通讯作者）
1.ZongyunChen,YoutianHu,JingHong,JunHu,WeishanYang,FangXiang,FanYang,ZiliXie,ZhijianCao,WenxinLi,DonghaiLin*,YingliangWu*.Toxinacidicresidueevolutionfunction-guideddesignofdenovopeptidedrugsforimmunotherapeutictargetKv1.3channel.ScientificReports,2015(inpress)
2.JingFeng,WeishanYang,ZiliXie,FangXiang,ZhijianCao,WenxinLiHongzhenHu,ZongyunChen*,YingliangWu*.KvchannelS1-S2linkerworkingasabindingsiteofhumanβ-defensin2forchannelactivationmodulation.JBiolChem,2015(inpress)

3.WeishanYang,JingFeng,FangXiang,ZiliXie,GuoyiZhang,Jean-MarcSabatier,ZhijianCao,WenxinLi,ZongyunChen*,YingliangWu*.Endogenousanimaltoxin-likehumanβ-defensin2inhibitsownK⁺channelsthroughinteractionwithchannelextracellularporeregion.CellularandMolecularLifeScience,2015,72:845-853

4.FangXiang,ZiliXie,WeishanYang,JingFeng,ZhijianCao,WenxinLi,ZongyunChen*,YingliangWu*.Plectasin,firstanimaltoxin-likefungaldefensingblockingpotassiumchannelsthroughrecognizingchannelporeregion.Toxins,2015,7:34-42

5.LingnaXu,TianLi,HonglianLiu,FanYang,SongpingLiang,ZhijianCao,WenxinLi,YingliangWu*.FunctionalcharacterizationoftwonovelscorpionsodiumchanneltoxinsfromLychasmucronatus.Toxicon,2014,90:318-325

6.ZongyunChen,YoutianHu,JunHu,WeishanYang,Jean-MarcSabatier,MichelDeWaard,ZhijianCao,WenxinLi,SongHan*,YingliangWu*.UnusualbindingmodeofscorpiontoxinBmKTXontopotassiumchannelsreliesonitsdistributionofacidicresidues.BiochemBiophysResCommun,2014,447(1):70-76

7.You-TianHu,JunHu,TianLi,JingjingWei,JingFeng,YimeiDu,ZhijianCao,WenxinLi*,YingliangWu*.OpenConformationofhERGChannelTurretsRevealedbyaSpecificScorpionToxinBmKKx2.Cell&Bioscience,2014,4:18

8.PanpanHou,RongZhang,YongfengLiu,JingFeng,WeiWang,YingliangWu^*andJiupingDing^*.PhysiologicalRoleofKv1.3ChannelinTLymphocyteCellInvestigatedQuantitativelybyKineticModeling.PLoSONE,2014,9(3):e89975

9.TianLi,LingnaXu,HonglianLiu,YawenHe,WenxinLi*,YingliangWu*.CharacterizationofanovelBmaTX47toxinmodulatingsodiumchannels:crucialroleofexpressionvectorsintoxinpharmacologicalactivity.Toxins,2014,6:816-829

10.CaoZJ,DiZY,WuYL*,LiWX*.OverviewofscorpionspeciesandtheirtoxinsfromChina.Toxins,2014,6:796-815

11.ChangXie,TianLi,LingnaXu,CongyaYu,ZhijianCao,WenxinLi*,YingliangWu*.Kv1.3potassiumchannel-blockingtoxinCtri9577,novelgatingmodifierofKv4.3potassiumchannelfromthescorpiontoxinfamily.BiochemBiophysResCommun,2014,444(3):406-410

12.JianMa,YoutianHu,MingxiongGuo,ZanHuang,WenxinLi*,YingliangWu*.hERGPotassiumChannelBlockagebyScorpionToxinBmKKx2EnhancesErythroidDifferentiationofHumanLeukemiaCellsK562.PLoSONE,2013,8(12):e84903

13.CaoZ^#,YuY^#,WuY^#,HaoP^#,DiZ^#,HeY,ChenZ,YangW,ShenZ,HeX,ShengJ,XuX,PanB,FengJ,YangX,HongW,ZhaoW,LiZ,HuangK,LiT,KongY,LiuH,JiangD,ZhangB,HuJ,HuY,WangB,DaiJ,YuanB,FengY,HuangW,XingX,ZhaoG,LiX*,LiY*,LiW*.ThegenomeofMesobuthusmartensiirevealsauniqueadaptationmodelofarthropods.NatCommun,2013,4:2602(并列第一作者)

14.JunHu,SuQiu,FanYang,ZhijianCao,WenxinLi,YingliangWu*.UniquemechanismoftheinteractionbetweenhoneybeetoxinTPNQandrKir1.1potassiumchannelexploredbycomputationalsimulations:Insightsintotherelativeinsensitivityofchanneltowardsanimaltoxins.PLoSONE,2013,8(7):e67213

15.JingFeng,YoutianHu,HongYi,ShijinYin,SongHan,JunHu,ZongyunChen,WeishanYang,ZhijianCao,MichelDeWaard,Jean-MarcSabatier,WenxinLi,YingliangWu*.TwoConservedArginineResiduesfromtheSK3PotassiumChannelOuterVestibuleControlsSelectivityofRecognitionbyScorpionToxins.JBiolChem,2013,288(18):12544-12553

16.ZongyunChen,FanLuo,JingFeng,WeishanYang,DanyunZeng,RuimingZhao,ZhijianCao,MailiLiu,WenxinLi*,LingJiang*,YingliangWu*.GenomicandStructuralCharacterizationofKunitz-typePeptideLmKTT-1aHighlightsDiversityandEvolutionofScorpionPotassiumChannelToxins.PLoSONE,2013,8(4):e60201

17.ChenZongyun,WangBin,HuJun,YangWeishan,CaoZhijian,ZhuoRuiming,LiWenxin*,WuYingliang*.SjAPI,theFirstFunctionallyCharacterizedAscaris-TypeProteaseInhibitorfromAnimalVenoms.PLoSONE.2013,8(3):e57529

18.ZhiLi,Wan-HongLiu,SongHan,Bi-WenPeng,JunYin,Ying-LiangWu*,Xiao-HuaHe*andWen-XinLi.SelectiveinhibitionofCCR^7-T_EMcellsactivationbyanovelpeptidetargetingKv1.3inaratEAEmodel.JBiolChem,2012,287:29479-29494

19.ZhenhuanZhao,WeiHong,ZhengyangZeng,YingliangWu,KanghongHu,XiaohuiTian,WenxinLi*andZhijianCao,*Mucroporin-M1inhibitshepatitisBvirusreplicationbyactivatingtheMAPKpathwayanddown-regulatingHNF4αinvitroandinvivo.JBiolChem,2012,287:30181-30190

20.ZYChen,DYZeng,YTHu,YWHe,NPan,JPDing,ZJCao,MLLiu,WXLi,HYi*,LJiang*,YLWu*.StructuralandFunctionalDiversityofAcidicScorpionPotassiumChannelToxins,PLoSONE,2012,7(4):e35154

21.Zong-YunChen,You-TianHu,Wei-ShanYang,Ya-WenHe,JingFeng,Bin-Wang,Rui-MingZhao,Jiu-PingDing,Zhi-JianCao,Wen-XinLi*,Ying-LiangWu*.Hg1,NovelPeptideInhibitorSpecificforKv1.3ChannelsfromFirstScorpionKunitz-typePotassiumChannelToxinFamily.JBiolChem,2012,287:13813-13821

22.HuiDai,ShijinYin,TianLi,ZhijianCao,YonghuaJi,YingliangWu*,WenxinLi*.Recombinantexpression,purification,andcharacterizationofscorpiontoxinBmαTX14.ProteinExpressionandPurification,2012,82:325-331

23.SuQiu,HongYi,JunHu,ZhijianCao,YingliangWu*andWenxinLi*.TheBindingModeofFusionInhibitorT20ontoHIV-1gp41andRelevantT20-ResistantMechanismsExploredbyComputationalStudy.CurrentHIVResearch,2012,10(2):182-194

24.ZhengFan,LuyangCao,YawenHe,JunHu,ZhiyongDi,YingliangWu,WenxinLi,*andZhijianCao*.Ctriporin,aNewAnti-Methicillin-ResistantStaphylococcusaureusPeptidefromtheVenomoftheScorpionChaerilustricostatus.AntimicrobialAgentsandChemotherapy,2011,55(11):5220-5229

25.LiQ,ZhaoZ,ZhouD,ChenY,HongW,CaoL,YangJ,ZhangY,ShiW,CaoZ,WuY,YanH*,LiW*.Virucidalactivityofascorpionvenompeptidevariantmucroporin-M1againstmeasles,SARS-CoVandinfluenzaH5N1viruses.Peptides,2011,32(7):1518-1525

26.Han,Song;Yin,Shijin;Yi,Hong;Mouhat,Stéphanie;Qiu,Su;Cao,Zhijian;Sabatier,Jean-Marc;Wu,Yingliang*;Li,Wenxin*.Protein-ProteinRecognitionControlbyModulatingElectrostaticInteractions.JournalofProteomeResearch,2010,9:3118-3125

27.YingWu,YuXiong,ShengWang,HongYi,HuiLi,NaPan,FrankTHorrigan,YingliangWu*,JiupingDing*.Inter-subunitcouplinginthePoreofBKchannels,JournalofBiologicalChemistry,2009,284(35):23353-23363

28.SuQiu,HongYi,HuiLiu,ZhijianCao,YingliangWu*,WenxinLi*.MolecularInformationofCharybdotoxinBlockadeintheLargeConductanceCalcium-activatedPotassiumChannel.JournalofChemicalInformationandModeling,2009,49(7):1831-1838

29.ShijinYin,LingJiang,HongYi,SongHan,DaiwenYang,MailiLiu,HuiLiu,ZhijianCao,YingliangWu*,WenxinLi*.DifferentresiduesinchannelturretdeterminingtheselectivityofADWX-1inhibitorpeptidebetweenKv1.1andKv1.3channels.JournalofProteomeResearch,2008,7(11):4890-4897

30.GeliangGan,HongYi,MaorongChen,LiangSun,WenxinLi,YingliangWu*andJiupingDing*.Structuralbasisfortoxin-resistanceofb4associatedcalcium-activatedpotassium(BK)channels.JournalofBiologicalChemistry,2008,283:24177-24184

31.SongHan,HongYi,Shi-JinYin,Zong-YunChen,HuiLiu,Zhi-JianCao,Ying-LiangWu*,andWen-XinLi*.StructuralBasisofaPotentPeptideInhibitorDesignedforKv1.3Channel,aTherapeuticTargetofAutoimmuneDisease.JournalofBiologicalChemistry,2008,283:19058-19065

32.YiH,QiuS,CaoZJ,WuYL*,LiWX*.MolecularbasisofinhibitorypeptidemaurotoxinrecognizingKv1.2channelexploredbyZDOCKandmoleculardynamicsimulations.PROTEINS:Structure,Function,andBioinformatics,2008,70:744-54

33.JinL*,WuY*.MolecularMechanismoftheSeaAnemoneToxinShKRecognizingtheKv1.3ChannelExploredbyDockingandMolecularDynamicSimulations.JournalofChemicalInformationandModeling,2007,47:1967-1972

34.HongYi,ZhijianCao,ShijinYin,ChaoDai,YingliangWu*,WenxinLi*.InteractionSimulationofhERGK⁺ChannelwithitsspecificBekm-1peptide:InsightsintotheSelectivityofMolecularRecognition.JournalofProteomeResearch,2007,6:611-620

35.YingliangWu,HongYi,ZhijianCao,WenxinLi.ConformationofTrimericEnvelopeGlycoproteins:TheCD4-dependentMembraneFusionMechanismofHIV-1.JournalofBiomolecularStructure&Dynamics,2007,25:1-9

36.YingliangWu,ZhijianCao,HongYi,DaheJiang,XinMao,HuiLiuandWenxinLi.SimulationoftheinteractionbetweenScyTxandsmallconductancecalcium-activatedpotassiumchannelbydockingandMM-PBSA.BiophysicalJournal,2004,87(1):105-112

37.Wu,YL;Hu,B*;Jiang,ZC;Chen,SZ.LowtemperaturevaporizationforICP-AESdeterminationofpalladiumingeologicalsamplesusingsampleintroductionofgaseouspalladiumoxinate.JournalofAnalyticalAtomicSpectrometry,2002,17(2):121-124

38.Wu,YL;Hu,B;Peng,TY;Jiang,ZC*.Low-temperaturevolatilizationofBeacetylacetonateforsampleintroductioninETV-ICP-AES.AnalyticalChimicaActa,2001,439(1):153-158