谢胜松
系所
基础兽医学系
职称
副教授
办公房间
动科楼三楼C座
email
ssxie@mail.hzau.edu.cn
讲授课程
主讲《动物生理学》和《动物生理学实验》，参与动物生理学MOOC和SPOC教 学，持续3年开设了本科生创新性实验教学项目《基因组编辑》。

个人简介 谢胜松，华中农业大学副教授，硕士研究生导师，2019年-2020年国际家畜研究所（ILRI）访问****。华中农业大学学士、农学硕士，中科院上海生命科学研究院生物化学与细胞生物学研究所理学博士。2013年4月至2014年11月为中科院生物化学与细胞生物学研究所助理研究员。现华中农业大学动科动医学院基础兽医系，农业动物遗传育种与繁殖教育部重点实验室固定成员，创新人才推进计划重点领域“猪基因组与育种创新团队”核心成员之一。 长期坚持在科研、教学一线，致力于科学研究、人才培养和平台建设。多年来，从事宿主和病原微生物功能基因挖掘、鉴定和机制解析研究，主要研究方向为：1）猪重要致病性病原微生物与宿主互作候选基因鉴定和功能研究，构建了基于猪全基因组CRISPR文库技术的高通量功能基因筛选平台；2）建立与优化基于CRISPR/Cas12系统的核酸可视化检测技术；3）CRISPR/Cas相关软件开发、技术优化及应用，基因编辑猪模型制备。目前共发表SCI论文34余篇，其中第一作者（含共一）5篇；通讯作者（含共同通讯）SCI文章6篇，单篇最高影响因子12.121（Nature Communications, 2020）。主持国家自然基金面上项目1项，校自主创新基金项目1项，作为研究骨干参加科技重大专项子课题1项；主持并结题国家自然基金青年项目1项。获得授权国家发明专利3项，软件著作权登记证书8个。
教育经历 2002.09 - 2006.06， 华中农业大学，动物科学专业毕业，农业学士
2006.09 - 2009.06， 华中农业大学，动物遗传育种与繁殖专业毕业，农学硕士
2009.09 - 2013.03， 中科院上海生命科学研究院生物化学与细胞生物学研究所，生物化学与分子生物学专业毕业，理学博士
工作经历 2013.04-2014.11， 中科院生物化学与细胞生物学研究所、助理研究员
2014.12-至今， 华中农业大学、副教授，硕士研究生导师
研究领域 猪重要致病性病原微生物与宿主互作，基因编辑技术，生物信息学软件与数据库开发。
科研成果 1.Zhao C, Liu H, Xiao T, Wang Z, Nie X, Li X, Qian P, Qin L, Han X, Zhang J, Ruan J, Zhu M, Miao Y, Zuo B, Yang K, Xie S*, Zhao S*. CRISPR screening of porcine sgRNA library identifies host factors associated with Japanese encephalitis virus replication. Nature Communications, 2020, 11:5178. (*Co-Corresponding authors)
2.Xiong Y, Han X, Zhang J, Zhao G, Wang Z, Zhuang R, Nie X, Xie S, Li C, Li X, Liu X, Li K, Zhao S, Ruan J. Identification of ACTB Gene as a Potential Safe Harbor Locus in Pig Genome. Mol Biotechnol, 2020, 26. doi: 10.1007/s12033-020-00276-6.
3.Tao D, Liu J, Nie X, Xu B, Tran-Thi TN, Niu L, Liu X, Ruan J, Lan X, Peng G, Sun L, Ma Y, Li X, Li C, Zhao SH, Xie S*. Application of CRISPR-Cas12a enhanced fluorescence assay coupled with nucleic acid amplification for the sensitive detection of African swine fever virus. ACS Synth Biol, 2020, 9(9):2339-2350.
4.Zhang S, Zhang K, Peng X, Zhan Hui, Lu Jia, Xie S, Zhao S, Li X, Ma Y. Selective sweep analysis reveals extensive parallel selection traits between Large white and Duroc. Evolutionary Applications, 2020, doi: 10.1111/eva.13085.
5.Zhan H, Zhang S, Zhang K, Peng X, Xie S, Li X, Zhao S, Ma Y. Genome-Wide Patterns of Homozygosity and Relevant Characterizations on the Population Structure in Piétrain Pigs. Genes (Basel), 2020, 11(5):577.
6.Chu C, Yu L, Henry-Berger J, Ru Y, Kocer A, Champroux A, Li Z, He M, Xie S, Ma W, Ni M, Ni Z, Guo Y, Fei Z, Gou L, Liu Q, Sharma S, Zhou Y, Liu M, Chen C, Eamens A, Nixon B, Zhou Y, Drevet J, Zhang Y. Knockout of glutathione peroxidase 5 down-regulates the piRNAs in the caput epididymidis of aged mice. Asian J Androl, 2020, doi: 10.4103/aja.aja_3_20.
7.Zhang D, Hussain A, Manghwar H, Xie K, Xie S, Zhao S, Larkin RM, Qing P, Jin S, Ding F. Genome editing with the CRISPR-Cas system: an art, ethics and global regulatory perspective. Plant Biotechnol J, 2020, 18(8):1651-1669.
8.Fu L, Wang H, Liao Y, Zhou P, Xu Y, Zhao Y, Xie S, Zhao S, Li X. miR-208b modulating skeletal muscle development and energy homoeostasis through targeting distinct targets. RNA Biol, 2020, 17(5):743-754.
9. Zhao C, Wang Y, Nie X, Han X, Liu H, Li G, Yang G, Ruan J, Ma Y, Li X, Cheng H, Zhao S, Fang Y*, Xie S*. Evaluation of the effects of sequence length and microsatellite instability on single-guide RNA activity and specificity. Int J Biol Sci, 2019, 15(12):2641-2653. (*Co-Corresponding authors)
10. Zheng B, Wang X, Liu Y, Li Y, Long S, Gu C, Ye J, Xie S, Cao S. Japanese Encephalitis Virus infection induces inflammation of swine testis through RIG-I—NF-?B signaling pathway.Veterinary Microbiology, 2019, 238:108430.
11. Han X, Xiong Y, Zhao C, Xie S, Li C, Li X, Liu X, Li K, Zhao S, Ruan J. Identification of Glyceraldehyde-3-Phosphate Dehydrogenase Gene as an Alternative Safe Harbor Locus in Pig Genome. Genes (Basel), 2019,10(9):660.
12. Gao H, Wen H, Cao C, Dong D, Yang C, Xie S, Zhang J, Huang X, Huang X, Yuan S, Dong W. Overexpression of MicroRNA-10a in Germ Cells Causes Male Infertility by Targeting Rad51 in Mouse and Human. Front Physiol, 2019, 10:765.
13. Zhao C, Xie S, Wu H, Luan Y, Hu S, Ni J, Lin R, Zhao S, Zhang D, Li X. Quantification of allelic differential expression using a simple fluorescence primer PCR-RFLP-based method. Sci. Rep, 2019, 9(1):6334.
14. Ma H, Zhang S, Zhang K, Zhan H, Peng X, Xie S, Li X, Zhao S, Ma Y. Identifying selection signatures for backfat thickness in Yorkshire pigs highlights new regions affecting fat metabolism. Genes (Basel), 2019, 10(4):254.
15. Xie S#, Zhu Q#, Qu W#, Xu Z, Liu X, Li X, Li S, Ma W, Miao Y, Zhang L, Du X, Dong W, Li H, Zhao C, Wang Y, Fang Y, Zhao S. sRNAPrimerDB: Comprehensive primer design and search web service for small non-coding RNAs. Bioinformatics, 2019, 35(9):1566-1572. (#Co-first authors)
16. Ma Y, Zhang S, Zhang K, Fang C, Xie S, Du X, Li X, Ni D, Zhao S. Genomic analysis to identify signatures of artificial selection and loci associated with important economic traits in Duroc pigs. G3 (Bethesda), 2018, 8(11):3617-3625.
17. Tang C, Ni M, Xie S, Zhang Y, Zhang C, Ni Z, Chu C, Wu L, Zhou Y, Zhang Y. DICER1 regulates antibacterial function of epididymis by modulating transcription of β-defensins. J. Mol. Cell Biol, 2018, 11(5):408-420.
18. Ma Y, Gu L, Yang L, Sun C, Xie S, Fang C, Gong Y, Li S. Identifying artificial selection signals in the chicken genome. PLoS One, 2018,13(4):e**.
19. Ahmad HI, Ahmad MJ, Asif AR, Adnan M, Iqbal MK, Mehmood K, Muhammad SA, Bhuiyan AA, Elokil A, Du X, Zhao C, Liu X*, Xie S*. A review of CRISPR-based genome editing: Survival, evolution and challenges. Curr. Issues Mol. Biol, 2018, 28:47-68. (*Co-Corresponding authors)
20. Zhang C, Zhou Y, Xie S, Yin Q, Tang C, Ni Z, Fei J, Zhang Y. CRISPR/Cas9-mediated genome editing reveals the synergistic effects of β-defensin family members on sperm maturation in rat epididymis. FASEB J, 2018, 32(3):1354-1363.
21. Cheng Y, Wei Z, Xie S, Peng Y, Yan Y, Qin D, Liu S, Xu Y, Li G, Zhang L. Alleviation of toxicity caused by overactivation of Pparα through Pparα-inducible miR-181a2. Molecular Therapy-Nucleic Acids, 2017, 9:195-206.
22. Zhao C, Zheng X, Qu W, Li G, Li X, Miao Y, Han X, Liu X, Li Z, Ma Y, Shao Q, Li H, Sun F*, Xie S*, Zhao S*. CRISPR-offinder: A CRISPR guide RNA design and off-target searching tool for user-defined protospacer adjacent motif. Int. J. Biol. Sci, 2017, 13(11):1470-1478. (*Co-Corresponding authors)
23. Xu J, Yao G, Ru Y, Xie S*. Expression of tamoxifen-inducible CRE recombinase in Lcn5-CreERT2 transgenic mouse caput epididymis. Mol. Reprod. Dev, 2017, 84(3):257-264.
24. Chen R, Du J, Ma L, Wang L, Xie S, Yang C, Lan X, Pan C, Dong W. Comparative microRNAome analysis of the testis and ovary of the Chinese giant salamander. Reproduction, 2017, 154(3):169-179.
25. Chu C, Zheng G, Hu S, Zhang J, Xie S, Ma W, Ni M, Tang C, Zhou L, Zhou Y, Liu M, Li Y, Zhang Y. Epididymal region-specific miRNA expression and DNA methylation and their roles in controlling gene expression in rats. PLoS One, 2015, 10(4):e**.
26.Apopo S, Liu H, Jing L, Du X, Xie S, Gong Y, Xu R, Li S. Identification and profiling of microRNAs associated with white and black plumage pigmentation in the white and black feather bulbs of ducks by RNA sequencing. Anim Genet, 2015,46(6):627-35.
27. Xie S, Shen B, Zhang C, Huang X, Zhang Y. sgRNAcas9: A software package for designing CRISPR sgRNA and evaluating potential off-target cleavage sites. PLoS One, 2014, 9(6):e100448.
28. Ma W, Hu S, Yao G, Xie S, Ni M, Liu Q, Gao X, Zhang J, Huang X, Zhang Y. An androgen receptor-microrna-29a regulatory circuitry in mouse epididymis. J. Biol. Chem, 2013, 288(41):29369-29381.
29. Xie S#, Xu J#, Ma W, Liu Q, Han J, Yao G, Huang X, Zhang Y. Lcn5 promoter directs the region-specific expression of cre recombinase in caput epididymidis of transgenic mice. Biol. Reprod, 2013, 88(3):71. (#co-first author)
30. Zhang J, Xie S, Ma W, Teng Y, Tian Y, Huang X, Zhang Y. A newly identified microRNA,mmu-miR-7578,functions as a negative regulator on inflammatory cytokines tumor necrosis factor-α and interleukin-6 via targeting Egr1 in vivo. J. Biol. Chem, 2013, 288(6):4310-20.
31. Sun N, Liu D, Chen H, Liu X, Meng F, Zhang X, Chen H, Xie S, Li X, Wu Z. Localization, expression change in PRRSV infection and association analysis of the porcine TAP1 gene. Int. J. Biol. Sci, 2012, 8(1):49-58.
32. Ma W, Xie S, Ni M, Huang X, Hu S, Liu Q, Liu A, Zhang J, Zhang Y. MicroRNA-29a inhibited epididymal epithelial cell proliferation by targeting nuclear autoantigenic sperm protein (NASP). J. Biol. Chem, 2012, 287(13):10189-99.
33. Xie S#, Li X#, Liu T, Cao J, Zhong Q, Zhao S. Discovery of porcine microRNAs in multiple tissues by a Solexa deep sequencing approach. PLoS One, 2011, 6(1):e16235. (#co-first author)
34. Xie S, Huang T, Shen Y, Li X, Zhang X, Zhu M, Qin H, Zhao S. Identification and characterization of microRNAs from porcine skeletal muscle. Anim. Genet, 2010, 41(2):179-90.?
科研项目 1. 基于CRISPR筛选发现的敲除猪CALR基因抑制乙型脑炎病毒增殖的抗病毒机制研究，国家自然科学基金面上项目，2021.01，58万，主持，进行中；
2. 基于聚焦型CRISPR/Cas9文库策略筛选与鉴定参与猪PRV感染的关键基因，中央高校基本科研业务费专项资金资助项目，2018.01，30万，主持，进行中；
3. 抗病高产转基因猪新品种培育，转基因生物新品种培育科技重大专项，2016.01，50万，研究骨干，进行中；
4. 小鼠附睾piRNA时空表达特性及其生物学功能研究，国家自然科学基金青年项目，2014.01，20万，主持，已结题。
主要奖励