姓名: 郭亮 硕/博导： 博导
性别: 男 开设课程：



普通遗传学，Plant Biology & Biotechnology



民族: 汉族 研究方向： 油菜油脂合成和油菜抗逆性的遗传基础和分子机制

职称: 教授 学位: 理学博士

联系方式

办公电话： (办公室)； (实验室)
电子邮件：guoliang@mail.hzau.edu.cn

个人简介

教授、博士生导师，作物遗传改良国家重点实验室PI，油菜遗传改良创新团队成员。2001-2006年先后获四川大学本科和硕士学位，2011年获美国密苏里大学圣路易斯校区细胞与分子生物学博士学位，2012-2014年在美国Donald Danforth Plant Science Center从事博士后研究，2014年10月受聘为华中农业大学教授。兼任农业农村部油菜遗传育种重点实验室副主任、Molecular Breeding执行主编、中国油料作物学报常务编委、湖北遗传学会理事、国际植物脂质会议委员会委员等。

课题组开展以下研究：

1. 油菜代谢生物学与遗传育种：1）油菜油脂合成的遗传基础和分子机制；2）磷脂、鞘脂代谢调控油菜生长、发育和抗逆的分子机制研究; 3）代谢组（脂质组）分析方法的建立与应用。

2. 油菜逆境生物学与遗传育种：耐盐碱、耐渍和抗旱性状的遗传基础和分子机制，抗逆种质资源筛选和利用。

3.抗除草剂油菜研发和产业化研究。课题组利用丰富的油菜种质资源，结合基因组、代谢组和表型组等手段全面解析油菜油脂合成与抗非生物逆境的遗传基础和分子机制，实现油菜优异种质资源→优异基因挖掘→优异基因功能解析→优异基因利用，为培育高含油量和抗逆强的油菜品种打下基础，将重要的QTL/基因应用到油菜的遗传改良中。本课题组计划招博士后2-3名，请将个人材料发至：guoliang@mail.hzau.edu.cn。

科研项目

1. 国家自然科学基金面上项目：利用脂质组分析解析油菜油脂合成的遗传基础，主持（2019-2022）
2. 转基因生物新品种培育重大专项“转基因油菜新品种培育及产业化研究”，主持 （2018-2020）
3. 作物遗传改良国家重点实验室自主课题：参与油脂合成的关键酶蛋白质结构解析，主持 （2018-2020）
4. 兵 团 重 大 科 技 项 目：南疆沙区盐碱地饲料（绿肥）油菜产业化关键技术研究与集成示范，项目骨干 （2018-2020）
5. 国家重大研发计划：“小麦等作物功能基因组研究与应用”，项目骨干 （2016-2020）
6. “国家外海高层次人才引进计划”青年人才项目，主持 （2016-2018）
7. 国家自然科学基金面上项目：植物鞘脂介导的信号传导研究，主持 （2016-2019）
8. 华中农业大学自主科技创新基金:基于脂质组分析解析油菜种子高含油量的分子机制，主持 （2015-2020）
9. 作物遗传改良国家重点实验室科研启动项目：植物脂质组分析方法的建立与应用，主持 （2015-2019）
10. 华中农业大学引进人才科研启动项目：植物脂质代谢与功能研究，主持 （2015-2019）

发明专利及获奖情况

1.湖北青年五四奖章 （2021）
2.华中农业大学第十六届研究生指导教师“教书育人奖”（2018）

3.“国家海外高层次引进人才”（青年项目）（2016）

4.Raju Mehra Awards (University of Missouri-St Louis, 2011)

发表的论文及著作

1. Yang B, Zhang K, Jin X, Yan J, Lu S, Shen Q, Guo L, Hong Y*, Wang X*, Guo L* (2021) Acylation of nonspecific phospholipase C4 determines its function in plant response to phosphate deficiency. Plant Journalhttps://doi.org/10.1111/tpj.15260.

2. Yang B^#, Li M^#, Phillips A, Li L, Ali U, Li Q, Lu S, Hong Y, Wang X* and Guo L* (2021) Non-specific phospholipase C4 hydrolyzes sphingophospholipids and sustains plant root growth under phosphate deficiency. Plant Cell https://doi.org/10.1093/plcell/koaa054.

3. Tang S^#, Zhao H^#, Lu S, Yu L, Zhang G, Zhang Y, Yang Q-Y., Zhou Y, Wang X, Ma W, Xie W* and Guo L* (2021) Genome- and transcriptome-wide association studies provide insights into the genetic basis of natural variation of seed oil content in Brassica napus. Molecular Plant 14:470-487.

4. Cui Y, Zeng X, Xiong Q, Wei D, Liao J, Xu Y, Chen G, Zhou Y, Dong H, Wan H, Liu Z, Li J, Guo L, Jung C, He Y*, Qian W* (2021) Combining quantitative trait locus and co-expression analysis allowed identification of new candidates for oil accumulation in rapeseed. Journal of Experimental Botany 72:1649-1660.

5. Chawla H, Lee H, Gabur I, amilselvan-Nattar-Amutha S, Obermeier C, Song J, Liu K, Guo L, Parkin I and Snowdon R* (2021) Long-read genome sequencing reveals widespread intragenic structural variants in a recent allopolyploid crop plant. Plant Biotechnol J 19:240-250.

6. Song J^#, Liu D^#, Xie W, Yang Z, Guo L, Liu K, Yang Q* and Chen L* (2021) BnPIR: Brassica napus Pan-genome Information Resource for 1,689 accessions. Plant Biotechnol J 19:412-414.

7. Luo T^#, Zhang Y^#, Zhang C, Nelson MN, Yuan J, Guo L and Xu Z* (2021) Genome-wide association mapping unravels the genetic control of seed vigor under low-temperature conditions in rapeseed (Brassica napus L.). Plants 10:426.

8. Tang S, Liu D, Lu S, Yu L, Li Y, Lin S, Li L, Du Z, Liu X, Li X, Ma W, Yang Q* and Guo L* (2020) Development and screening of EMS mutants with altered seed oil content or fatty acid composition in Brassica napus. Plant Journal 104:1410-1422.

9. Dai C, Li Y, Li L, Du Z, Lin S, Tian X, Li S, Yang B, Yao W, Wang J, Guo L* and Lu S* (2020) An efficient Agrobacterium-mediated transformation method using hypocotyl as explants for Brassica napus. Molecular Breeding 40:96.

10. Kong Q, Yang Y, Low PM, Guo L, Yuan L and Ma W* (2020) The function of the WRI1-TCP4 regulatory module in lipid biosynthesis. Plant Signaling & Behavior 15:**.

11. 杜卓霖，郭亮，鲁少平* (2020) 甘蓝型油菜皖油12种子中含芥酸含甘油三酯的空间分布. 油料作物学报.

12. Kong Q, Singh S, Mantyla J, Pattanaik S, Guo L, Yuan L, Benning C and Ma W* (2020) TEOSINTE BRANCHED1/CYCLOIDEA/PROLIFERATING CELL FACTOR 4 interacts with WRINKLED1 to mediate seed oil biosynthesis. Plant Physiology 184:658-665.

13. Kim SC, Guo L and X Wang* (2020) Nuclear moonlighting of cytosolic glyceraldehyde-3-phosphate dehydrogenase regulates Arabidopsis response to heat stress. Nature Communications 11:3439.

14. Dai C, Wu J, Yan G, Duan Z, Wang Z, Kang C, Guo L, Liu K, Tu J, Shen J, Yi B, Fu T, Li X and Ma C* (2020) Roles of Brassica napus DELLA Protein BnaA6.RGA in modulation of drought tolerance by interacting one of ABA signaling component BnaA10.ABF2. Frontiers in Plant Science 11:577.

15. You L^#, Zhang J, Li L, Xiao C, Feng X, Chen S, Guo L, and Honghong Hu* (2020) Involvement of abscisic acid, ABI5, and PPC2 in plant acclimation to low CO₂. Journal of Experimental Botany 71:4093-4108.

16. Cai G, Fan C, Liu S, Yang Q, Liu D, Wu J, Li J, Zhou Y, Guo L* and X Wang* (2020) Nonspecific Phospholipase C6 Increases Seed Oil Production in Oilseed Brassicaceae Plants. New Phytologist 226:1055-1073.

17. Sturtevant D^#, Lu S^#, Zhou Z^#, Shen Y^#, Wang S, Song J, Zhong J, Burks D, Yang Z, Yang Q, Cannon A, Herrfurth C, Feussner I, Borisjuk L, Munz E, Verbeck G, Wang X, Azad R, Singleton B, Dyer J, Chen L*, Chapman K* and Guo L* (2020) The genome of jojoba (Simmondsia chinensis): a taxonomically-isolated species that directs wax-ester accumulation in its seeds. Science Advances 6:eaay3240.

18. Song J^#, Guan Z^#, Hu J^#, Guo C, Yang Z, Wang S, Liu D, Wang B, Lu S, Zhou R, Xie W, Cheng Y, Zhang Y, Liu K*, Yang Q*, Chen L* and Guo L* (2020) Eight high-quality genomes reveal pan-genome architecture and ecotype differentiation of Brassica napus. Nature Plants 6:34-45.

19. Lu S^#, Aziz M^#, Sturtevant D, Chapman K* and Guo L* (2020) Heterogeneous distribution of erucic acid in Brassica napus seeds. Frontiers in Plant Science 10:1744.

20. Iqbal S^#, Ali U^#, Fadlalla T, Li Q, Liu H, Lu S* and Guo L* (2020) Genome-wide characterization of phospholipase A & C families and pattern of lysolipids and diacylglycerol changes under abiotic stresses in Brassica napus L. Plant Physiology and Biochemistry 147:101-112.

21. Kong Q, Yang Y, Guo L, Yuan L and Ma W* (2020) Molecular basis of plant oil biosynthesis: insights gained from studying the WRINKLED1 transcription factor. Frontiers in Plant Science 11:24.

22. Li N, Meng H, Li S, Zhang Z, Wang S, Liu A, Li Q, Zhao X, Song Q, Li X, Guo L, Li H, Zuo J and Luo K* (2020) Two novel plastid fatty acid exporters contribute to seed oil accumulation in Arabidopsis. Plant Physiology 182:1910-1919.

23. Ding L^#, Li M^#, Guo X^#, Tang M^#, Cao J, Wang Z, Zhu K, Guo L, Liu S* and Tan X* (2020) Arabidopsis GDSL1 overexpression enhances rapeseed Sclerotinia sclerotiorum resistance and the functional identification of its homolog in Brassica napus. Plant Biotechnol J 18:1255-1270.

24. Chen B^#, Zhang G^#, Li P, Yang J, Guo L, Benning C, Wang X and Zhao J* (2020) Multiple GmWRI1s are redundantly involved in seed filling and nodulation by regulating plastidic glycolysis, lipid biosynthesis, and hormone signaling in soybean (Glycine max). Plant Biotechnol J 18:155-171.

25. Lu S^#, Liu H^#, Jin C, Li Q and Guo L* (2019) An Efficient and Comprehensive Plant Glycerolipids Analysis Approach Based on High-performance Liquid Chromatography-quadrupole Time of Flight Mass Spectrometer. Plant Direct 3:1-13.

26. Zhang Y^#, Ali U^#, Zhang G, Yu L, Fang S, Iqbal S, Li H, Lu S and Guo L* (2019) Transcriptome analysis reveals genes commonly responding to multiple abiotic stresses in rapeseed. Molecular Breeding 39:158.

27. Lu S^#, Fadlalla T^#, Tang S, Li L, Ali U, Li Q and Guo L* (2019) Genome-wide analysis of phospholipase D gene family and profiling of phospholipids under abiotic stresses in Brassica napus. Plant and Cell Physiology 60:1556-1566.

28. 张宇婷，鲁少平，金诚，郭亮* (2019) 甘蓝型油菜皖油20号种子不同部位油脂合成的转录调控分析. 作物学报 45: 381-389.

29. Li N^#, *, Zhang Y^#, Meng H^#, Li S, Wang S, Xiao Z, Chang P, Zhang X, Li Q, Guo L, Igarashi Y and Luo F* (2019) Characterization of Fatty Acid EXporters involved in fatty acid transport for oil accumulation in the green alga Chlamydomonas reinhardtii. Biotechnology for Biofuels 12:14.

30. Xie K*, Guo L, Bai Y, Liu W, Yan J and Bucher M* (2019) Microbiomics and Plant Health: An Interdisciplinary and International Workshop on the Plant Microbiome. Molecular Plant 12: 1-3.

31. Ali U, Li H, Wang X and Guo L* (2018) Emerging Roles of Sphingolipid Signaling in Plant Response to Biotic and Abiotic Stresses. Molecular Plant 11: 1328-1343.

32. Lu S, Sturtevant D, Aziz M, Jin C, Li Q, Chapman K* and Guo L* (2018) Spatial analysis of lipid metabolites and expressed genes reveals tissue-specific heterogeneity of lipid metabolism in high- and low-oil Brassica napus L. seeds. Plant Journal 94: 915-932.

33. Ali M, Hussain R, Rehman N, She G, Li P, Wan X and Guo L* and Zhao J* (2018) De novo transcriptome sequencing and metabolite profiling analyses reveal the complex metabolic genes involved in the terpenoid biosynthesis in Blue Anise Sage. DNA Research 25: 597-617.

34. Su Y, Li M, Guo L and Wang X* (2018) Different effects of phospholipase Dζ2 and nonspecific phospholipase C4 on lipid remodeling and root hair growth in Arabidopsis response to phosphate deficiency. Plant Journal 94: 315-326.

35. Zhang Q, Berkey R, Blakeslee J, Lin J, Ma X, King H, Liddle A and Guo L, Munnik T, Wang X, Xiao S* (2018) Arabidopsis phospholipase Dα1 and Dδ oppositely modulate EDS1- and SA-independent basal resistance against adapted powdery mildew. Journal of Experimental Botany 69: 3675-3688.

36. Rehman N, Ali M, Ahmad M, Guo L* and Zhao J* (2018) Strigolactones promote rhizobia interaction and increase nodulation in soybean (Glycine max). Microbial Pathogenesis 114: 420-430.

37. Zhang Q, Song P, Qu Y, Wang P, Jia Q and Guo L, Zhang C, Mao T, Yuan M, Wang X and Zhang W* (2017) Phospholipase Dδ negatively regulates plant thermotolerance by destabilizing cortical microtubules in Arabidopsis. Plant, Cell & Environment 40: 2220-2235.

38. Wei F, Fanella B, Guo L* and Wang X* (2016) Membrane glycerolipidome of soybean root hairs and its response to nitrogen and phosphate availability. Scientific Reports 6: 36172.

39. Hong Y*, Zhao J*, Guo L, Kim S, Deng X, Wang G, Zhang G, Li M and Wang X* (2016) Plant phospholipases D and C and their diverse functions in stress responses. Progress in Lipid Research 62: 55-74.

40. Lu S*, Yao S, Wang G, Guo L, Zhou Y, Hong Y and Wang X* (2016) Phospholipase Dε enhances Braasca napus growth and seed production in response to nitrogen availability. Plant Biotechnol J 14:926-937.

41. Wu J, Zhao Q, Yang Q, Liu H, Li Q, Yi X, Cheng Y, Guo L, Fan C and Zhou Y* (2016) Comparative transcriptomic analysis uncovers the complex genetic network for resistance to Sclerotinia sclerotiorum in Brassica napus. Scientific Reports 6:19007.

42. Guo L, Ma F, Wei F, Fanella B, Allen D and Wang X* (2014) Cytosolic Glyceraldehyde-3-Phosphate Dehydrogenase Affects Arabidopsis Cellular Metabolism and Promotes Seed Oil Accumulation. Plant Cell 26:3023-3035.

43. Wang X, Guo L, Wang G and Li M (2014) PLD: Phospholipase Ds in Plant Signaling, Phospholipases in Plant Signaling, Springer: 3-26 (Book chapter).

44. Yao H, Wang G, Guo L and Wang X* (2013) Phosphatidic Acid Interacts with WEREWOLF MYB and Regulates Its Nuclear Localization and Function in Arabidopsis. Plant Cell 25:5030-5042.

45. Kim S, Guo L and Wang X* (2013) Phosphatidic Acid Binds to Cytosolic Glyceraldehyde-3-Phosphase Dehydrogenase and Promotes Its Cleavage in Arabidopsis. J Biol Chem 288:11834-11844.

46. Guo L, Devaiah S, Narasimhan R, Pan X, Zhang Y, Zhang W and Wang X* (2012) Cytosolic glyceraldehyde-3-phosphate dehydrogenases interact with phospholipase Dδ to transduce hydrogen peroxide signals in stress response in Arabidopsis. Plant Cell 24:2200-2212.

47. Guo L, Mishra G, Markham J, Li M, Tawfall A, Welti R and Wang X* (2012) Connections between sphingosine kinase and phospholipase D in the abscisic acid signaling pathway in Arabidopsis. J Biol Chem 287:8286-8296.

48. Guo L and Wang X* (2012) Crosstalk between phospholipase D and sphingosine kinase in plant stress signaling. Frontiers in Plant Science 3:1-7.

49. Guo L, Mishra G, Taylor K and Wang X* (2011) Phosphatidic acid binds and stimulates Arabidopsis sphingosine kinases. J Biol Chem 286:13336-13345.

50. Li M, Bahn S, Guo L, Musgrave W, Berg H, Welti R and Wang X* (2011) Patatin-related phospholipase pPLAIIIbeta-induced changes in lipid metabolism alter cellulose content and cell elongation in Arabidopsis. Plant Cell 23:1107-1123.

51. Guo L, Qing R, He W, Xu Y, Tang L, Wang S and Chen F* (2008) Identification and characterization of a plastidial ω3-fatty acid desaturase gene from Jatrophacurcas. Chin J Appl Environ Biol 14:469-474.

52. Niu B, Guo L, Zhao M, Luo T, Zhang R, Zhang F, Hou P, Zhang Y, Xu Y, Wang S and Chen F* (2008) Molecular cloning, characterization, and expression of an ω-3 fatty acid desaturase gene from Sapium sebiferum. J Biosci Bioeng 106:375-380.

53. 何玮，郭亮，王岚，杨威，唐琳，陈放* (2007) 麻疯树种质资源遗传多样性的 ISSR 分析. 应用与环境生物学报 13: 466-470.54. Xiao M, Li Q, Wang L, Guo L, Li J, Tang L, Xu Y and Chen F* (2006) ISSR analysis of genetic diversity and genetic structure in natural populations of an endangered species Sinopodophyllum hexandrum (Royle) Ying from the Tibetan region of

Sichuan province, China. J Integr Plant Biol 48:1140-1146.

55. Li Q, Xiao M, Guo L, Wang L, Tang L, Xu Y, Yan F and Chen F* (2005) Genetic diversity and genetic structure of an endangered species, Trillium tschonoskii. Biochemical Genetics 43:445-458.

56. Xiao M, Li Q, Guo L, Luo T, He W, Wang L and Chen F* (2005) AFLP analysis of genetic diversity of the endangered species Sinopodophyllum hexandrum (Royle) Ying in the Tibetan region of Sichuan province, China. Biochemical Genetics 44:47-60.

57. 李群，肖猛，郭亮，李静，段文霞，陈放*，王丽 (2005) 四川省珍稀濒危植物延龄草遗传多样性分析. 北京林业大学学报 27:1-6.

58. 戎芳，王胜华，赵小光，郭亮，魏琴，唐琳，徐莺，陈放* (2005) 麻疯树外植体愈伤组织中毒蛋白的 Westem-blot 鉴定. 四川大学学报: 42:206-209.