更新日期：2018年9月6日
姓 名 Marco Pistolozzi 性 别 男
出生年月 1978年8月 籍贯
民 族 外国血统 政治面貌
最后学历 博士研究生 最后学位 理学博士
技术职称 副教授 导师类别 硕导
行政职务Email marco_pistolozzi@scut.edu.cn
工作单位 生物科学与工程学院 邮政编码 510006
通讯地址 华南理工大学大学城校区B6办公楼
单位电话 **
个人主页 http://kygl.7i3.cas.scut.edu.cn:80/zhuye/**


工作经历
2008/10 – 2011/04，意大利博洛尼亚大学，药物科学学院, 博士后。
2010/10 – 2011/03，英国利物浦大学，电气工程和电子学院，生物纳米工程实验室，访问学者
2012/03 至今，华南理工大学，医药生物学专业，副教授
教育经历
2004年，毕业于意大利博洛尼亚大学，药物化学与技术, 5年（学士 + 硕士学位）
2008年，英国伦敦国王学院药剂学院，光学光谱实验室, 访问学者, 6个月
2008年，毕业于意大利博洛尼亚大学, 药物科学博士学位
研究领域
主要研究药理学的分子互动特性（包括药物-蛋白质/蛋白质-蛋白质相互作用，蛋白质和肽的聚集，酶动力学, 等等）。科研中所使用到的波谱技术（紫外-可见分光光度技术，圆二色谱，荧光），色谱技术（直接相手性色谱，反相色谱法，亲和层析法），亲和技术（表面等离子体共振）和质谱等。
科研项目
2012–2016, 广东省重大科技专项, 广东华南中医药技术创新与产业服务平台, 参与
2013-2015, 广州市科技计划项目, 重大科技, 抗心肌缺血植物单体一类新药的临床前研究, 参与
2014–2015, 中央高校基本科研业务费专项资金资助, 基于伪不可逆结合从复杂蛋白质组中钓取丝氨酸水解酶, 负责人
2014–2015, 年度广东省发酵与酶工程重点实验室开放基金, 采用班布特罗单氨基甲酸酯探针选择性的分离酶, 负责人
2018-2021广州市科技计划项目, 创新环境建设计划, 珠江科技新星专题, 基于固相萃取-超临界流体色谱-质谱联用技术的脑卒中脂质组学研究, 主要完成人
发表论文
1. X. Yang, M. Pistolozzi, Z. Lin*. New trends in aggregating tags for therapeutic protein purification. Biotechnol Lett 40(5), 745-753, 2018. doi: 10.1007/s10529-018-2543-2.

2. H. Su, M. Pistolozzi, X. Shi, X. Sun*, W. Tan*. Alterations in NO/ROS ratio and expression of Trx1 and Prdx2 in isoproterenol-induced cardiac hypertrophy. Acta Biochim Biophys Sin (Shanghai) 49(11), 1022-1028, 2017. doi: 10.1093/abbs/gmx102.

3. J. Shang, X. Dai, Y. Li, M. Pistolozzi, L. Wang*. HybridSim-VS: a web server for large-scale ligand-based virtual screening using hybrid similarity recognition techniques. Bioinformatics 33(21), 3480-3481, 2017. doi: 10.1093/bioinformatics/btx418.

4. Q. Xiao, H. Zhou, H. Wei, H. Du, W. Tan, Y. Zhan, M. Pistolozzi*. A new method to characterize the kinetics of cholinesterases inhibited by carbamates. J Pharm Biomed Anal 144, 175-182, 2017. doi: 10.1016/j.jpba.2017.04.007.

5. H. Zhou, Q. Xiao, W. Tan, Y. Zhan, M. Pistolozzi*. Quantitative estimation of cholinesterase-specific drug metabolism of carbamate inhibitors provided by the analysis of the area under the inhibition-time curve. J Pharm Biomed Anal 144, 167-174, 2017. doi: 10.1016/j.jpba.2017.04.008.

6. J. Wu, Y. Tian, S. Wang, M. Pistolozzi, Y. Jin, T. Zhou, G. Roy, L. Xu, W. Tan*. Design, synthesis and biological evaluation of bambuterol analogues as novel inhibitors of butyrylcholinesterase. Eur J Med Chem 126, 61-71, 2017.

7. K. Shi, G. Chen, M. Pistolozzi, F. Xia, Z. Wu*. Improved analysis of monascus pigments based on their ph-sensitive uv-vis absorption and reactivity properties. Food Addit Contam Part A Chem Anal Control Expo Risk Assess 33(9), 1396-1401, 2016.

8. K. Shi, D. Song, G. Chen, M. Pistolozzi, Z. Wu*, L. Quan. Controlling composition and color characteristics of Monascus pigments by pH and nitrogen sources in submerged fermentation. J Biosci Bioeng 120(2), 145-54, 2015.

9. M. Pistolozzi, H. Du, H. Wei, W. Tan*.Stereoselective inhibition of human butyrylcholinesterase by the enantiomers of bambuterol and their intermediates. Drug Metab Dispos 43, 344-352, 2015.

10. D. Tedesco, M. Pistolozzi, R. Zanasi, C. Bertucci*.Characterization of the species-dependent ketoprofen/albumin binding modes by induced CD spectroscopy and TD-DFT calculations. J Pharm Biomed Anal 112, 176-80, 2015.

11. M. Pistolozzi, C. Fortugno, C. Franchini, F. Corbo, M. Muraglia, M. Roy, G. Felix, C. Bertucci*. Species-dependent binding of tocainide analogues to albumin: affinity chromatography and circular dichroism study. J Chrom B 968, 69-78, 2014.

12. T. Zhou, Q.Cheng, C. Zou, T. Zhao, S. Liu, M. Pistolozzi, E. Tan, L. Xu, W. Tan*. Simultaneous determination of bambuterol and its two major metabolites in human plasma by hydrophilic interaction ultra-performance liquid chromatography-tandem mass spectrometry. J Chrom B 967, 225-234, 2014.

13. L. Huang, B. Chen, M. Pistolozzi, Z. Q. Wu*, J. F. Wang. Inoculation and alkali coeffect in volatile fatty acids production and microbial community shift in the anaerobic fermentation of waste activated sludge. Biores Technol 153, 87-94, 2014.

14. M. Pistolozzi, V. Royo, A. C. Pereira, M. L. Silva, R. Silva, W. R. Cunha, K. Vaconcelos, Q. B. Cass, C. H. Martins, J. K. Bastos, G. Varchi, A. Guerrini, C. Bertucci*. Enantiomeric HPLC resolution and absolute stereochemistry assignment of a new poligamain derivative. J Pharm Biomed Anal, 75, 118-22, 2013.

15. M. Naldi, J. Fiori, M. Pistolozzi, A. F. Drake, C. Bertucci, R. L. Wu, K. Mlynarczyk, S. Filipek, A. De Simone, V. Andrisano*. Amyloid β-peptide 25?35 self-assembly and its inhibition: a model undecapeptide system to gain atomistic and secondary structure details of the Alzheimer’s disease process and treatment. ACS Chem Neurosci, 3(11), 952-962, 2012.

16. D. I. Pérez*, M. Pistolozzi, V. Palomo, M. Redondo, C. Fortugno, C. Gil, G. Felix, A. Martinez, C. Bertucci. 5-imino-1,2-4-thiadiazoles and quinazolines derivatives as glycogen synthase kinase 3β (GSK-3β) and phosphodiesterase 7 (PDE7) inhibitors: determination of blood brain barrier penetration and binding to human serum albumin. Eur J Pharm Sci 45(5), 677-684, 2012.

17. C. Bertucci*, M. Pistolozzi, D. Tedesco, R. Zanasi, R. Ruzziconi, A. M. Di Pietra. Stereochemical characterization of fluorinated 2-(phenanthren-1-yl)propionic acids by enantioselective HPLC analysis and electronic circular dichroism detection. J Chrom A 1232, 128-133, 2012.

18. M. Bartolini*, M. Pistolozzi, V. Andrisano, J. Egea, M. G. López, I. Iriepa, I. Moraleda, E. Gálvez, J. Marco-Contelles*, A. Samadi. Chemical and pharmacological studies on enantiomerically pure p-methoxytacripyrines, promising multi-target-directed ligands for the treatment of Alzheimer’s disease. ChemMedChem 6(11), 1990-1997, 2011.

19. C. Bertucci*, A. De Simone, M. Pistolozzi, M. Rosini. Reversible HSA binding of lipocrine: a circular dichroism study. Chirality, 23(9), 827-832, 2011

20. M. Pistolozzi, G. Varchi, A. Degli Esposti, A. Guerrini, G. Sotgiu, M. Ballestri, C. Ferroni, A. Venturini, C. Bertucci*. Camptothecin and thio-camptothecin: the role of sulfur in shifting the hydrolysis equilibrium towards the closed lactone form. ChemMedChem 6(9), 1706-1714, 2011.

21. C. Bertucci*, M. Pistolozzi, A. De Simone. Structural characterization of recombinant therapeutic proteins by circular dichroism. Curr Pharm Biotechnol 12(10), 1508-1516, 2011.

22. M. Pistolozzi, C. Bertucci, A. De Simone, F. Mancini, V. Andrisano. Characterization of BACE-1 inhibition mechanism by circular dichroism and fluorescence spectroscopy. J Biotechnol 150(S1), S467, 2010.

23. O. Prezzavento*, A. Campisi, C. Parenti, S. Ronsisvalle, G. Aricò, E. Arena, M. Pistolozzi, G. M. Scoto, C. Bertucci, A. Vanella, G. Ronsisvalle. Synthesis and resolution of cis-(±)-methyl (1R,2S/1S,2R)-2-[(4-hydroxy-4-phenylpiperidin-1-yl)methyl]-1-(4-methylphenyl)cyclopropanecarboxylate [(±)-PPCC)]: new σ receptor ligands with neuroprotective effect. J Med Chem 53(15), 5881-5885, 2010.

24. C. Bertucci*, M. Pistolozzi, A. De Simone. Circular dichroism in drug discovery and development: an abridged review. Anal Bional Chem 398(1), 155-166, 2010.

25. C. Ghelardini, J. F. Desaphy, M. Muraglia, F. Corbo, R. Matucci, A. Dipalma, C. Bertucci, M. Pistolozzi, M. Nesi, M. Norcini, C. Franchini, D. C. Camerino. Effects of a new potent analog of tocainide on HNAV1.7 sodium channels and in vivo neuropathic pain models. Neuroscience 169(2), 863-873, 2010.

26. M. Naldi, M. Pistolozzi, C. Bertucci, A. De Simone, S. Altilia, M. Pierini, C. Franceschi, S. Salvioli, V. Andrisano*. Structural characterization of p53 isoforms due to the polymorphism at codon 72 by mass spectrometry and circular dichroism. J Pharm Biomed Anal 53(2), 200-206, 2010.

27. M. Pistolozzi, C. Franchini, F. Corbo, M. Muraglia, M. De Giorgi, G. Felix, C. Bertucci*. Tocainide analogues binding to Human Serum Albumin: a HPLAC and circular dichroism study. J Pharm Biomed Anal 53(2), 179-185, 2010.

28. C. Bertucci, M. Pistolozzi, G. Felix, U.H. Danielson. HSA binding of HIV protease inhibitors: a high-performance affinity chromatography study. J Sep Sci 32(10), 1625-1631, 2009.

29. M. Pistolozzi, C. Bertucci. Species-dependent stereoselective drug binding to albumin: a circular dichroism study. Chirality, 20(3-4), 552-558, 2008.

30. C. Bertucci, A. Piccoli, M. Pistolozzi. Optical biosensors as a tool for early determination of adsorption and distribution parameters of lead candidates and drugs. Comb Chem High Throughput Screen 10(6), 433-440, 2007.

31. L. Guandalini, M. Norcini, K. Varani, M. Pistolozzi, C. Gotti, C. Bazzicalupi, E. Martini, S. Dei, D. Manetti, S. Scapecchi, E. Teodori, C. Bertucci, C. Ghelardini and M.N. Romanelli. Design, synthesis, and preliminary pharmacological evaluation of new quinoline derivatives as nicotinic ligands. J Med Chem 50(20), 4993-5002, 2007.
出版专著和教材
C. Bertucci, M. Pistolozzi. ELECTRONIC CIRCULAR DICHROISM IN DRUG DISCOVERY, in “Comprehensive chiroptical spectroscopy vol. 2: applications in stereochemical analysis of synthetic compounds, natural products, and biomolecules”, part IV, chapter 25, Eds. N. Berova, P. L. Polavarapu, K. Nakanishi, R. W. Woody, 2012, Hoboken, NJ, Wiley-Blackwell (Wiley & Sons, Inc.), ISBN-10: **; ISBN-13: **54.
教学活动
承担全英文硕士课程《蛋白质化学与药物-蛋白质相互作用研究》, 以及生物制药专业本科课程《药理学》。
指导学生情况
指导过的研究生人数共8名, 已毕业7名