一.个人简况
1965年生,博士, 教授,博士生导师,福建农林大学作物科学学院副院长。福建省科技创新领军人才,福建省“百千****才工程”培养对象,福建农林大学“金山****”创新人才,《Plant Cell & Environment》、《Journal of Experiment Botany》、《Phytopathology》、《园艺学报》、《基因组学与应用生物学》等国内外刊物审稿专家,国家自然基金通讯评审专家。
教育背景:
1984/9-1988/7江西农业大学农学系,学士
1988/9-1991/7华中农业大学作物栽培与耕作学专业,硕士
1994/9-1998/7福建农业大学作物栽培与耕作学专业,博士
(1996/10-1998/1韩国国立庆北大学农学院,博士学位论文研究)
工作经历:
1991/7-1994/7原福建农学院农学系,助教
1994/9-1999/7原福建农业大学农学系,讲师
1999/9-2003/7福建农林大学作物学院,副教授
2003/9-2004/10福建农林大学作物学院,教授,博士生导师
2004/10-2009/9福建农林大学生命科学学院,教授,博士导师,副院长
2008/7-2009/1美国加州大学河滨分校植物与植物科学系,访问****
2009/9-2011/9福建农林大学海外学院,教授,博士生导师,主持工作副院长
2011/9--福建农林大学作物学院,教授,博士生导致,副院长
二. 研究领域和方向:
研究领域:
植物生物技术与分子生物学;蔬菜遗传改良与新品种选育,蔬菜设施在无公害栽培
主要研究方向:
1、蔬菜及水稻抗逆功能基因组学研究
(1)应答逆境逆境信号通路的分子剖析
植物抗病及抗逆是通过对病原菌侵染及逆境胁迫信号的感知、信号传导及相关基因表达的改变来实现的,受到复杂的信号传递网络的调控。本实验室长期以来围绕着植物应答逆境胁迫的信号传导网络的分子剖析,以茄科蔬菜辣椒为主要材料,瞄准辣椒在高温高湿下病害发生严重这一重要生产问题,着重研究辣椒应答疫霉及青枯菌等主要病原菌及高温高湿逆境的信号通路交互作用(crosstalk)中小G蛋白、激酶、转录因子(WRKY、NAC、ERF等)等重要节点的结构和功能,提出茄科植物应答高温高湿和抗病偶联的假说,发现了CaROP1、CaWRKY6和CaWRKY6等在辣椒协调应答病原菌侵染及高温高湿逆境过程中起重要的调节作用。
(2)辣椒与疫霉协同进化的分子机制研究
大量的研究发现植物在与病原菌协同进化过程中形成了PTI和ETI彼此交互作用的两个层次抗病机制,但这两个层次的抗病反应之间存在着信号通路和防御反应的重叠,暗示PTI和ETI之间存在着连续分布的中间态。我们以辣椒疫霉的系列非典型分子模式(PAMP)或非典型效应子(effector)为研究对象,发现由SRC2-1和SGT1等参与的辣椒应对elicitin的之字型协同进化关系。
2、辣椒、白萝卜遗传改良
以高温高湿下辣椒等作物抗病分子机制及相关分子标记开发研究为基础,开展分
子标记、杂交育种、生态压力选择等相结合的辣椒等蔬菜高温高湿下抗病种质资源创新和新品种选育,已经选育出闽椒1号、闽椒2号、闽椒3号和闽椒4号以及金玉3号等蔬菜新品种经过省级认定,开展了其高产高效配套栽培技术研究,并在福建及江西等地示范推广应用。
3、蔬菜无公害生产关键技术研究和推广
本方向包括:
(1)诱导抗病小分子化合物筛选与在作物上应用,与美国加州大学河滨分校Thomas Eulgem教授实验室合作,利用对病原菌高效持续高效应答基因启动子与GUS报告基因的转基因植株,建立诱导抗病小分子化合物高通量筛选体系,目前已经筛选获得10种以上小分子化合物,其中DCA在茄科蔬菜和水果荔枝保鲜中表现出较大的应用潜力;
(2)蔬菜育苗基质研发,已经利用木屑等原料,研发出一种高效、廉价的蔬菜育苗基质;
(3)茄科蔬菜涝害恢复技术,通过应用抗病小分子化合物和生长调节剂,通过优化配方,研发出辣椒等茄科植物涝害淹水后的抗病恢复技术;
(4)研究了在闽东南沿海地区青花菜菜田的主要害虫发生规律,并建立了其可持续防治的技术体系。
三.承担的主要课题
1.辣椒含DWE的WRKY基因功能相关及其在协同应答高温及青枯菌中作用解析,国家自然基金,(**),2016-2019
2.CaWRKY40介导辣椒耐高温高湿及抗青枯病分子机制解析,国家自然科学基金,**,2014-01-2017.12,80万元。
3.辣椒高温高湿下抗病功能基因组学研究及高产优质高抗辣椒设施型新品种选育与应用,福建省重大科技专项,66万元,2013.10-2016.12.
4.抗病转基因水稻新品种培育,国家转基因生物新品种培育重大专项,2009ZX08001-015B,65万元,2009-2012
5. 激活水稻LURM基因表达化合物筛选及其诱导抗病活性分析,国家自然科学基金,**,2010-2012,30万元
6. CaWRKY5激活植物低温抗性的结构基础和分子机制研究,福建省自然科学基金重点项目,2008J0003,2008-2011,20万元
7. 辣椒种质资源评价与优良自交系及强优势组合的选配,福建省科技重点项目,2008N0099,10万元
8.设施性专用系列新品种创新及产业化工程,福建省种业创新与产业化工程项目,2014-2015,50万元。
9. 闽椒1号产业化关键技术研究与示范推广,福建省农业五新工程项目,25万元
10. 闽椒2号的推广应用,福建省农业科技跨越计划,8万元。
四. 2012年以来发表的通讯作者论文(仅列出Sci论文)
1.The Ectopic Expression of CaRop1 Modulates the Response of Tobacco Plants to Ralstonia solanacearumand Aphids.Front Plant Sci,2016, 7, 1177. IF=4.495
2.A novel leucine-rich repeat protein, CaLRR51, plays as a positive regulator in pepper's response to Ralstonia solanacearum infection Mol Plant Pathol,2016, DOI:10.1111/mpp.12462.IF= 4.335.
3.Pepper CabZIP63 acts as a positive regulator during Ralstonia solanacearum or high temperature–high humidity challenge in a positive feedback loop withCaWRKY40.Journal of Experimental Botany,2016,DOI: 10.1093/jxb/erw069,IF= 5.677
4.CaCDPK15 positively regulates pepper responses to Ralstonia solanacearum inoculation and forms a positive-feedback loop with CaWRKY40 to amplify defense signaling. Scientific Reports,2016, DOI: srep22439 [pii]10.1038/srep22439, IF=5.578
5.SGT1 is required in PcINF1/SRC2-1induced pepper defense responseby interacting with SRC2-1. Scientific Reports,2016, DOI:srep21651 [pii]10.1038/srep21651, IF=5.578
6.CaWRKY6 Functions as Transcriptional Activator of CaWRKY40, Synergistically Regulating
Pepper Resistance to Ralstonia solanacearumand Thermotolerance. Journal of Experimental
Botany,2015,DOI:10.1093/jxb/erv125 ,IF= 5.677
7.Use of enhancer trapping to identify pathogen-induced regulatory events spatially restricted to
plant-microbe interaction sites. Mol Plant Pathol,DOI:10.1111/mpp.12287, IF = 4.724
8.SRC2-1 is required in PcINF1-induced pepper immunity by acting as an interacting partner of PcINF1. Journal of Experimental Botany,2015, DOI:10.1093/jxb/erv161,IF= 5.677
9. Genome-wide Identification and Transcriptional Expression Analysis ofMAPK and MAPKK Genes in Capsicum annuum.Frontiers in Plant Science,2015, DOI:10.3389/fpls.2015.00780,IF=3.948
10.Identification of CDPK and CDPK-related protein kinase (CRK) genes in pepper. Frontiers in Plant Science.2015, DOI:10.3389/fpls.2015.00737,IF=3.948
11. Over-Expression of Rice CBS Domain Containing Protein,OsCBSX3, Confers Rice Resistance to Magnaporthe oryzae Inoculation.Int. J. Mol. Sci. 2015, 16, 1-x; DOI:10.3390/ijms160x000x,IF= 2.862
12. CaWRKY40, a WRKY protein of pepper, plays an important role in the regulation of tolerance to heat stress and resistance to Ralstonia solanacearum infection, Plant Cell Environ,2013,36(4):757-774,IF=5.906,高被引论文.
13. CaWRKY58, encoding a group I WRKY transcription factor of Capsicum annuum, negatively regulates resistance to Ralstonia solanacearuminfection,Mol Plant Pathol,2013,14(2):131-144, IF= 4.485
14.Overexpression of a pepper CaERF5 gene in tobacco plants enhances resistance toRalstonia solanacearuminfection. Functional Plant Biology,2014, 41 (7):758-767, (IF= 3.145) 3区
15. Overexpression of CaWRKY27, a subgroup IIe WRKY transcription factor of Capsicum annuum, positively regulates tobacco resistance to Ralstonia solanacearuminfection. Physiol Plant,2014,15(3): 397-411, IF= 3.138
16. Functional Analysis and Expressional Characterization of Rice Ankyrin Repeat-Containing Protein, OsPIANK1, in Basal Defense against Magnaporthe oryzae Attack. Plos one,2013,8(3) e59699,IF=3.77
17. Overexpression of a Chinese cabbage BrERF11 transcription factor enhances disease resistance to Ralstonia solanacearum in tobacco,Plant Physiology and Biochemistry,2013,62:70-78, IF= 2.83
18. Differential gene expression in pepper (Capsicum annuum) exposed to UV-B,Indian J Exp Biol,2011,49(6):429-437, IF= 1.295
五、发明专利
1.一种辣椒DN突变体在烟草抗高温基因工程中的应用,(申请号:CN5.2 授权公告号: B )
2.一种辣椒DN突变体及其载体的构建和应用,(申请号:CN4.1 授权公告号: B)
3.含W盒的诱导型启动子及构建和在基因工程上的应用,(申请号:CN5.6 授权公告号: B)
4.含S盒的诱导型启动子及构建和在基因工程上的应用,(申请号:CN1.4 授权公告号: B)
5.含GCC盒的诱导型启动子及构建和在基因工程上的应用,(申请号:CN2.9 授权公告号: B)
6.含JERE盒的诱导型启动子及构建和在基因工程上的应用,(申请号:CN3.3 授权公告号: B)
7.一种辣椒DN突变体在烟草抗青枯病基因工程中的应用,(申请号:CN2.2 授权公告号: B)
8.辣椒CaWRKY40基因在烟草抗青枯病基因工程中的应用,(申请号:CN1.4 )
9.辣椒CaWRKY40基因在烟草耐高温基因工程中的应用,(申请号:CN1.8)
10.一种辣椒AP2/ERF转录因子基因及其应用,(申请号:CN2.3 )
11.一种新型蔬菜育苗复合基质及其制备方法,(申请号:CN7.3 )
12.一种白菜AP2/ERF转录因子基因及其应用,(申请号:CN 0.0)
六、育成品种
1.闽椒1号,福建省非主要农作物新品种认定委员会认定,2010
2.闽椒2号,福建省非主要农作物新品种认定委员会认定,2010
3.金玉3号,福建省非主要农作物新品种认定委员会认定,2012
4.闽椒3号,福建省非主要农作物新品种认定委员会认定,2016
5.闽椒4号,福建省非主要农作物新品种认定委员会认定,2016
七、鉴定成果
1.具有保健功能胡萝卜新种质资源创建与应用,经福建省科技厅组织成果鉴定,达同类研究的国际先进水平
2.应答低温的CaWRKY5功能及其结构基础研究,经福建省科技厅组织的成果鉴定,达同类研究的国内领先水平
八、出版教材和专著
1.植保素代谢与植物防御反应,广东科技出版社,2002年(独著)
2.基因工程,科学出版社,2008年(主编)
3.农业总论,中国农业大学出版社(面向二十一世纪教材)(参编)
九.联系方式
1.E-mail:shlhe201304@aliyun.com
2.联系电话:;**
Vitae
Name:Shuilin HE
Unit:
Title:Professor, Associated Dean
Profile:
Dr. He received his Bachelor degreein Agronomy from
Research work:
Functional genomics of plant stress physiology
Publications:
1.The Ectopic Expression of CaRop1 Modulates the Response of Tobacco Plants to Ralstonia solanacearumand Aphids.Front Plant Sci,2016, 7, 1177. IF=4.495
2.A novel leucine-rich repeat protein, CaLRR51, plays as a positive regulator in pepper's response to Ralstonia solanacearum infection Mol Plant Pathol,2016, DOI:10.1111/mpp.12462.IF= 4.335.
3.Pepper CabZIP63 acts as a positive regulator during Ralstonia solanacearum or high temperature–high humidity challenge in a positive feedback loop withCaWRKY40.Journal of Experimental Botany,2016,DOI: 10.1093/jxb/erw069,IF= 5.677
4.CaCDPK15 positively regulates pepper responses to Ralstonia solanacearum inoculation and forms a positive-feedback loop with CaWRKY40 to amplify defense signaling. Scientific Reports,2016, DOI: srep22439 [pii]10.1038/srep22439, IF=5.578
5.SGT1 is required in PcINF1/SRC2-1induced pepper defense responseby interacting with SRC2-1. Scientific Reports,2016, DOI:srep21651 [pii]10.1038/srep21651, IF=5.578
6.CaWRKY6 Functions as Transcriptional Activator of CaWRKY40, Synergistically Regulating
Pepper Resistance to Ralstonia solanacearumand Thermotolerance. Journal of Experimental
Botany,2015,DOI:10.1093/jxb/erv125 ,IF= 5.677
7.Use of enhancer trapping to identify pathogen-induced regulatory events spatially restricted to
plant-microbe interaction sites. Mol Plant Pathol,DOI:10.1111/mpp.12287, IF = 4.724
8.SRC2-1 is required in PcINF1-induced pepper immunity by acting as an interacting partner of PcINF1. Journal of Experimental Botany,2015, DOI:10.1093/jxb/erv161,IF=5.677
9. Genome-wide Identification and Transcriptional Expression Analysis ofMAPK and MAPKK Genes in Capsicum annuum.Frontiers in Plant Science,2015, DOI:10.3389/fpls.2015.00780,IF=3.948
10.Identification of CDPK and CDPK-related protein kinase (CRK) genes in pepper. Frontiers in Plant Science.2015, DOI:10.3389/fpls.2015.00737,IF=3.948
11. Over-Expression of Rice CBS Domain Containing Protein,OsCBSX3, Confers Rice Resistance to Magnaporthe oryzae Inoculation.Int. J. Mol. Sci. 2015, 16, 1-x; DOI:10.3390/ijms160x000x, IF= 2.862
12. CaWRKY40, a WRKY protein of pepper, plays an important role in the regulation of tolerance to heat stress and resistance to Ralstonia solanacearum infection, Plant Cell Environ,2013,36(4):757-774,IF=5.906
13. CaWRKY58, encoding a group I WRKY transcription factor of Capsicum annuum, negatively regulates resistance to Ralstonia solanacearuminfection,Mol Plant Pathol,2013,14(2):131-144, IF= 4.485
14.Overexpression of a pepper CaERF5 gene in tobacco plants enhances resistance toRalstonia solanacearuminfection. Functional Plant Biology,2014, 41 (7):758-767, (IF= 3.145) 3区
15. Overexpression of CaWRKY27, a subgroup IIe WRKY transcription factor of Capsicum annuum, positively regulates tobacco resistance to Ralstonia solanacearuminfection. Physiol Plant,2014,15(3): 397-411, IF= 3.138
16. Functional Analysis and Expressional Characterization of Rice Ankyrin Repeat-Containing Protein, OsPIANK1, in Basal Defense against Magnaporthe oryzae Attack. Plos one,2013,8(3) e59699,IF=3.77
17. Overexpression of a Chinese cabbage BrERF11 transcription factor enhances disease resistance to Ralstonia solanacearum in tobacco,Plant Physiology and Biochemistry,2013,62:70-78, IF= 2.83
18. Differential gene expression in pepper (Capsicum annuum) exposed to UV-B,Indian J Exp Biol,2011,49(6):429-437, IF= 1.295
Contact:
1.Phone:86-, Mobile phone: 86-**
2.E-mail:shlhe201304@aliyun.com