汪凯
学术学位硕士生导师

副研究员



wangkai.virus@qq.com

感染性疾病分子生物学重点实验室
http://kaiwang.academic.site




学科信息


个人简介
汪凯，博士，副研究员，硕士生导师。2012年毕业于中国科学院上海巴斯德研究所，获理学博士学位。硕博连读期间主要从事病毒离子通道研究及抗病毒药物筛选；博士后工作期间主要负责传染性疾病分子诊断试剂研发。2015年就职于重庆医科大学，目前主要从事分子病毒学及肝癌代谢研究；围绕代谢与信号转导的交互调控，旨在揭示代谢重编程在肝癌发生发展中的作用，为肝癌治疗提供新的靶点。先后主持2项国家自然科学基金项目；2项重庆市科委及教委课题，1项重庆医科大学“新冠肺炎”应急课题；获国家发明专利1项。在国内外期刊发表40余篇论文，其中以第一作者或通讯作者（含共同）在Cell Research、EMBO Journal、Journal of Clinical Investigation、Cell Death & Differentiation、Clinical Infectious Diseases、Signal Transduct Target Ther.和Oncogene等期刊发表SCI论文20余篇，被引用1000余次，谷歌学术H-index为16。
ORCiD: https://orcid.org/0000-0002-0137-1247


工作经历：
2020.10- 至今重庆医科大学 感染性疾病分子生物学重点实验室 副研究员
2019.10- 2020.9 重庆医科大学 感染性疾病分子生物学重点实验室 副教授
2015.9 -2019.9 重庆医科大学 基础医学院 病原生物学教研室讲师
2013.7 - 2015.7 中国科学院 上海生命科学研究院博士后
2012.5 - 2013.5 中科院上海巴斯德研究所 分子病毒与免疫重点实验室 助理研究员


研究方向：
(1)分子病毒学；
(2)代谢重编程调控肝癌发生发展的分子机制研究。


学术任职：
中国抗癌协会肿瘤代谢专委会青年委员；
Advanced Science、Genes & Disease、Clinical and Translational Medicine、Frontiers in Oncology、Frontiers in Cellular and Infection Microbiology等学术期刊审稿人。


科研项目

国家自然科学基金 (面上项目)，PCK1通过降低O-GlcNAc糖基化修饰抑制肝癌转移的机制研究，主持（在研）。
重庆医科大学“新型冠状病毒肺炎”应急研究专项，SARS-CoV-2假病毒系统的建立及其在抗病毒药物筛选中的应用，主持（已结题）。
重庆市教育委员会雏鹰计划研究项目，糖异生关键酶抑制肝癌细胞增殖和迁移能力初探，主持（已结题）。
重庆市教委科学技术研究计划，PCK1下调β-catenin稳定性抑制肝癌EMT的分子机制研究，主持（在研）。
国家自然科学基金 (青年基金)，PCK1通过Wnt信号通路抑制肝细胞癌的作用机制研究，主持 （已结题）。



论文情况

Jin Xiang^#, Chang Chen^#, Rui Liu^#, Dongmei Gou^#, Lei Chang, Haijun Deng, Qingzhu Gao, Wanjun Zhang, Lin Tuo, Xuanming Pan, Li Liang, Jie Xia, Luyi Huang, Ke Yao, Bohong Wang, Zeping Hu, Ailong Huang*, Kai Wang*, Ni Tang*, Gluconeogenic enzyme PCK1 deficiency promotes CHK2 O-GlcNAcylation and hepatocellular carcinoma growth upon glucose deprivation. Journal of Clinical Investigation, 2021, 131(8):e144703. (*Co-corresponding author, IF=14.808)
Pai Peng^#, Hai-jun Deng^#, Jie Hu^#, Xiao-yu Wei, Jian-jiang Xue, Ting-ting Li, Liang Fang, Bei-zhong Liu, Ai-shun Jin, Feng-li Xu, Kang Wu, Quan-xin Long, Juan Chen, Kai Wang*, Ni Tang*, Ai-long Huang*, Humoral responses in naive or SARS-CoV-2 experienced individuals vaccinated with an inactivated vaccine. Cell Discovery, 2021, 7:68. (*Co-corresponding author, IF= 10.849)
Kai Wang^#, Quan-Xin Long^#, Hai-Jun Deng^#, Jie Hu^#, Qing-Zhu Gao, Gui-Ji Zhang, Chang-long He, Lu-Yi Huang, Jie-li Hu, Juan Chen*, Ni Tang*, Ai-Long Huang*, Longitudinal Dynamics of the Neutralizing Antibody Response to Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) Infection. Clinical Infectious Diseases, 2021. 73(3):e531-e539. (Co-first author, IF=9.079)
Qiujie Wang^#, Bin Cheng^#, Qiang Xue^#, Qingzhu Gao, Ailong Huang*, Kai Wang*, Ni Tang*, GSTZ1 sensitizes hepatocellular carcinoma cells to sorafenib-induced ferroptosis via inhibition of NRF2/GPX4 axis. Cell Death & Disease, 2021,12(5):426. (*Co-corresponding author, IF=8.469)
Yi Liu^#, Haijun Deng^#, Li Liang, Guiji Zhang, Jie Xia, Keyue Ding, Ni Tang*, Kai Wang*, Depletion of VPS35 attenuates metastasis of hepatocellular carcinoma by restraining the Wnt/PCP signaling pathway. Genes & Diseases, 2021, 8(2): 232-240. (*Co-corresponding author，IF=7.103)
Chang-Long He^#, Lu-Yi Huang^#, Kai Wang^#, Chen-Jian Gu, Jie Hu, Gui-Ji Zhang, Wei Xu, You-Hua Xie*, Ni Tang*, Ai-Long Huang*, Identification of bis-benzylisoquinoline alkaloids as SARS-CoV-2 entry inhibitors from a library of natural products. Signal Transduction and Targeted Therapy, 2021, doi : 10.1038/s41392-021-00531-5. (#Co-first author, IF=18.187)
Jie Hu^# , Pai Peng^#, Kai Wang^#, Liang Fang, Fei-yang Luo, Ai-shun Jin, Bei-zhong Liu*, Ni Tang*, Ai-long Huang*, Emerging SARS-CoV-2 variants reduce neutralization sensitivity to convalescent sera and monoclonal antibodies. Cellular & Molecular Immunology, 2021, doi: 10.1038/s41423-021-00648-1 (#Co-first author, IF=11.53)
Pai Peng^#, Jie Hu^#, Hai-jun Deng, Bei-zhong Liu, Liang Fang, Kai Wang*, Ni Tang*, Ai-long Huang*, Changes of humoral immunity response in SARS-CoV-2 convalescent patients over 8 months. Cellular & Molecular Immunology, 2021, 18(2), 490–491. (*Co-corresponding author, IF=11.53)

Jie Hu^#, Qingzhu Gao^#, Changlong He, Ailong Huang, Ni Tang*, Kai Wang*, Development of cell-based pseudovirus entry assay to identify potential viral entry inhibitors and neutralizing antibodies against SARS-CoV-2. Genes & Diseases, 2020, 7(4):551-557. (*Co-corresponding author，IF=7.103)
Jie Hu^#, Qingzhu Gao^#, Yang Yang^#, Jie Xia^#, Wanjun Zhang, Yao Chen, Zhi Zhou, Lei Chang, Yuan Hu, Hui Zhou, Li Liang, Xiaosong Li, Quanxin Long, Kai Wang*, Ailong Huang*, Ni Tang*, Hexosamine biosynthetic pathway promotes the antiviral activity of SAMHD1 by enhancing O-GlcNAc transferase-mediated protein O-GlcNAcylation. Theranostics, 2021, 11(2):805-823. (*Co-corresponding author, IF=11.556)
Qingzhu Gao^#, Guiji Zhang^#, Yaqiu Zheng^#, Yi Yang^#, Chang Chen, Jie Xia, Li Liang, Chong Lei, Yuan Hu, Xue-Fei Cai, Wenlu Zhang, Hua Tang, Yaxi Chen, Ailong Huang*, Kai Wang*, Ni Tang*, SLC27A5 deficiency activates NRF2/TXNRD1 pathway by increased lipid peroxidation in HCC. Cell Death & Differentiation, 2020, 27(3):1086–1104.(*Co-corresponding author, IF=15.828)
Guiji Zhang^#, Xia Tang^#, Li Liang^#, Wanfeng Zhang, Dewei Li, Xiaoyuan Li, Dachun Zhao, Yaqiu Zheng, Yanhong Chen, Bingtao Hao, Kai Wang*, Ni Tang*, Keyue Ding*, DNA and RNA sequencing identified a novel oncogene VPS35 in liver hepatocellular carcinoma, Oncogene, 2020, 39(16):3229-3244. (*Co-corresponding author, IF=9.867)
Jin Xiang^#, Yuhong Zhang^#, Lin Tuo, Rui Liu, Dongmei Gou, Li Liang, Chang Chen, Jie Xia, Ni Tang, Kai Wang*, Transcriptomic changes associated with PCK1 overexpression in hepatocellular carcinoma cells detected by RNA-Seq. Genes & Diseases, 2020,7(1):150-159. (Corresponding author，IF=7.103)
Chong Lei^#, Qiujie Wang, Ni Tang*, Kai Wang*, GSTZ1-1 downregulates Wnt/β-catenin signalling in hepatocellular carcinoma cells. FEBS Open Bio, 2020, 10(1):6-17. (Corresponding author，IF=2.693)
Jingjing Li^#, Qiujie Wang^#, Yi Yang, Chong Lei, Fan Yang, Li Liang, Chang Chen, Jie Xia, Kai Wang*, Ni Tang*, GSTZ1 deficiency promotes hepatocellular carcinoma proliferation via activation of the KEAP1/NRF2 pathway. Journal of Experimental & Clinical Cancer Research, 2019, 38(1):438. (*Co-corresponding author, IF=11.161)
Fan Yang^#, Jingjing Li^#, Haijun Deng^#, Yihao Wang, Chong Lei, Qiujie Wang, Jin Xiang, Li Liang, Jie Xia, Xuanming Pan, Xiaosong Li, Quanxin Long, Lei Chang, Ping Xu, Ailong Huang*, Kai Wang*, Ni Tang*, GSTZ1-1 Deficiency Activates NRF2/IGF1R Axis in HCC via Accumulation of Oncometabolite Succinylacetone. The EMBO Journal, 2019, 38(15):e101964. (*Co-corresponding author, IF=11.598)
Xuemei Chen^#, Yuan Hu^#, Wenlu Zhang, Ke Chen, Jie Hu, Xiaosong Li, Li Liang, Xuefei Cai, Jieli Hu, Kai Wang*, Ailong Huang*, Ni Tang*, Cisplatin induces autophagy to enhance hepatitis B virus replication via activation of ROS/JNK and inhibition of the Akt/mTOR pathway. Free Radical Biology and Medicine, 2019, 131: 225-236. (*Co-corresponding author, IF=7.376)
Lin Tuo^#, Jin Xiang^#, Xuanming Pan^#, Jieli Hu, Hua Tang, Li Liang, Jie Xia, Yuan Hu, Wenlu Zhang, Ailong Huang*, Kai Wang*, Ni Tang*, PCK1 negatively regulates cell cycle progression and hepatoma cell proliferation via the AMPK/p27^Kip1 axis. Journal of Experimental & Clinical Cancer Research, 2019, 38(1):50.(*Co-corresponding author, IF=11.161)
Lin Tuo^#, Jin Xiang^#, Xuanming Pan, Qingzhu Gao, Guiji Zhang, Yi Yang, Li Liang, Jie Xia, Kai Wang*, Ni Tang*, PCK1 Downregulation Promotes TXNRD1 Expression and Hepatoma Cell Growth via the Nrf2/Keap1 Pathway. Frontiers in Oncology, 2018, 8: 611. (*Co-corresponding author, IF=6.244)
Qingzhu Gao^#, Kai Wang^#, Ke Chen, Li Liang, Yaqiu Zheng, Yunzhi Zhang, Jin Xiang, Ni Tang *, HBx protein-mediated ATOH1 downregulation suppresses ARID2 expression and promotes hepatocellular carcinoma. Cancer Science, 2017, 108(7): 1328-1337. (#Co-first author)
Kai Wang^#, Wei Lv^#, Jianfei Chen^#, Shiqi Xie, Hongyan Shi, Haojen Hsu, Wenjing Yu, Ke Xu, Chao Bian, Wolfgang B Fischer, Wolfgang Schwarz, Li Feng*, Bing Sun*, PEDV ORF3 encodes an ion channel protein and regulates virus production. FEBS Letters, 2012, 586(4):384-391.
Kai Wang^#, Shiqi Xie, Bing Sun*. Viral proteins function as ion channels. Biochimica et Biophysica Acta (BBA)-Biomembranes, 2011, 1808(2): 510-515.
Shiqi Xie^#, Kai Wang^#, Wenjing Yu, Wei Lv, Ke Xu, Jianwei Wang, Bin Ye, Wolfgang Schwarz, Qi Jin*, Bing Sun*, DIDS blocks a chloride-dependent current that is mediated by the 2B protein of enterovirus 71, Cell Research, 2011, 21(8): 1271-1275. (#Co-first author)
Ronghua Zhang^#, Kai Wang^#, Wei Lv^#, Wenjing Yu, Shiqi Xie, Ke Xu, Wolfgang Schwarz, Sidong Xiong*, Bing Sun*, The ORF4a protein of human coronavirus 229E functions as a viroporin that regulates viral production. Biochimica et Biophysica Acta (BBA)-Biomembranes, 2014, 1838(4): 1088-1095. (#Co-first author)
Ronghua Zhang^#, Kai Wang, Xianqiang Ping, Wenjing Yu, Zhikang Qian, Sidong Xiong*, Bing Sun*, The ns12. 9 accessory protein of human coronavirus OC43 is a viroporin involved in virion morphogenesis and pathogenesis. Journal of Virology, 2015, 89(22): 11383-11395.
Silvia Schwarz^#, Kai Wang, Wenjing Yu, Bing Sun, Wolfgang Schwarz*, Emodin inhibits current through SARS-associated coronavirus 3a protein. Antiviral Research, 2011, 90(1): 64-69.
Silvia Schwarz^#, Daniel Sauter, Kai Wang, Ronghua Zhang, Bing Sun, Anastasia Karioti, Anna Rita Bilia, Thomas Efferth, Wolfgang Schwarz*, Kaempferol derivatives as antiviral drugs against the 3a channel protein of coronavirus. Planta medica, 2014, 80(02/03): 177-182.
Daniel Sauter^#, Silvia Schwarz, Kai Wang, Ronghua Zhang, Bing Sun, Wolfgang Schwarz*, Genistein as antiviral drug against HIV ion channel. Planta medica, 2014, 80(08/09): 682-687.
潘璇明^#，张桂冀，陈雪梅，梁利，唐霓，汪凯*. 果糖-1，6-二磷酸酶1抑制肝癌细胞株HepG2的自噬及增殖. 中华肝脏病杂志, 2019,27( 9 ): 687-692.
向进^#，庹琳，高庆祝，杨翼，梁利，夏杰，唐霓，汪凯*. PCK1影响肝癌细胞迁移能力的研究.重庆医科大学学报,2018, 43(5), 624-628.


知识产权
汪凯，唐霓，庹琳，向进，潘璇明. 6-重氮-5-氧代-L-正亮氨酸在制备预防或治疗肝癌药物中的应用，申请号 CN3.9
唐霓，汪凯，黄爱龙，夏杰，向进，庹琳，潘璇明，刘芮，苟冬梅. 氨基氧乙酸在制备治疗肝细胞肝癌的药物中的应用，授权号 ZL4.0
黄露义，唐霓，汪凯，黄爱龙，何长龙. 双苄四氢异喹啉衍生物在制备抗冠状病毒药物中的应用, 申请号 CN3.9
黄露义，唐霓，汪凯，黄爱龙，杨泱. N-磺酰基杂环衍生物及其制药用途, 申请号 CN6.1.
夏杰，黄爱龙，刘迪娜，唐霓，汪凯，蔡莹，沙雨，潘敏刚. 一种肝纤维化或肝硬化的诊断试剂盒， 申请号 CN7.6







研究方向
分子病毒学；代谢重编程调控肝癌发生发展的分子机制研究。