硕导个人简介


u个人简介
廖飞，教授，硕士生导师。重庆市优秀中青年骨干教师、重庆市学术技术带头人后备人选、教育部新世纪优秀人才。J Fluo、Biosens Bioelectron、Sensors and Actuators B: Chemical等杂志审稿人。1986.09~1990.06，兰州大学生物系生物化学专业获理学学士学位；1990.09~1993.06，兰州大学生物系生物物理学专业获理学硕士学位。1993.07~2017.06，重庆医科大学教师；2017.07~，重庆理工大学药学与生物工程学院教师。作为项目负责人，主持科技部863项目1项、国家自然科学基金5项、教育部新世纪优秀人才支持计划1项、重庆市科委重点项目1项。作为通讯作者，在SCI收录杂志发表论文56篇，他引超过260次，h-index达 16。作为第一发明人，获中国发明专利授权18项，美国发明专利1项，转化应用3项。作为副主编参编卫生部八年制临床医学教材1部、参编科学出版社本科教材1部。
研究领域
药物筛选及体外诊断的分析新方法、酶和蛋白质突变体设计筛选
蛋白配体设计和筛选、分离及分析微纳米材料
u承担的主要项目
[1] 酶反应动力学过程分析的应用，国家自然科学青年基金，2003.1-2005.12，19万，主持。
[2] 混合物中靶蛋白高亲合力配体集群筛选方法的建立和应用，国家自然科学基金面上项目，2005.1-2007.12，20万，主持。
[3] 苛求芽孢杆菌胞内尿酸酶的克隆表达、分子改造和应用，国家自然科学基金面上项目，2007.1-2009.12，28万，主持。
[4]教育部新世纪优秀人才支持计划，教育部，2010.1-2012.12，50万，主持。
[5] 以蛋白质芳香族氨基酸残基为共振能量转移供体的均相亲和分析方法，国家自然科学基金面上项目，2011.1-2013.12，32万，主持。
[6] 新型免疫检测技术的研究，科技部863项目，100万，主持。
[7]季也蒙毕赤酵母尿酸酶和苛求芽孢杆菌尿酸酶最适pH影响因素的比较和应用，国家自然科学基金面上项目，2018.1-2021.12，58.5万，主持。

u代表性成果（通讯作者论文及第一发明人授权专利）
[1]Ampholytic ion-exchange materials coated with small zwitterions for high-efficacy purification of ionizable soluble biomacromolecules. Int J Biol Macromol. 2018. doi: 10.1016/j.ijbiomac.2018.08.020.
[2]High-throughput estimation of specific activities of enzyme/mutants in cell lysates through immunoturbidimetric assay of proteins. Anal Biochem 2017, 534(1):91-98.
[3]Achievement of linear response for competitive bioaffinity assay of ligand: criteria of optimized interaction system. RSC Adv 2016, 6(112): 110858-110865.
[4]Extracorporeal delivery of a therapeutic enzyme. Sci Report 2016; 6:30888.
[5]Crystal structure of Bacillus fastidious uricase reveals an unexpected folding of the C-terminus residues crucial for thermostability under physiological conditions. Appl Micriobiol Biotechnol 2015; 99(19):7973-7986.
[6]Approximated maximum adsorption of His-tagged enzyme/mutants on Ni2+-NTA for comparison of specific activities. Int J Biol Macromol 2015; 74: 211-217.
[7]Microplate-based method to screen inhibitors of isozymes of cyclic nucleotide phosphodiesterase fused to SUMO. J Enzyme Inhibit Med Chem2014, 29(6):836-839.
[8]Facile quantitative comparison of specific activities of fusion-tagged enzyme/mutants in cell lysates via prediction of their maximum adsorption by anti-tag antibody immobilized in microplate wells. RSC Advance 2014; 4(56): 29925-29932.
[9]Fluorometric titration approach for calibration of quantity of binding site of purified monoclonal antibody recognizing epitope/hapten nonfluorescent at 340 nm. Anal Chem 2014, 86(12):5667-5672.
[10]Selective and sensitive homogenous assay of serum albumin with 1-anilinonaphthalene-8-sulphonate as a biosensor. Anal Chim Acta 2014, 829(1):60-67.
[11]Resonant-Mie-scattering of aggregates of phosphomolybdate and papaverine for measuring activities and screening inhibitors of cyclic nucleotide phosphodiesterase isozymes. Anal Chim Acta 2013, 804(1):215-220.
[12]Spectrophotometric-dual-enzyme-simultaneous-assay in one reaction solution (SDESA): chemometrics and experimental models. Anal Chem 2013, 85 (4):2143-2154.
[13]Facile one-step coating approach to magnetic submicron particles with poly(ethylene glycol) coats and abundant accessible carboxyl groups. Int J NanoMed2013, 8:791-807.
[14]Classification of difference between inhibition constants of an inhibitor to facilitate identifying the inhibition type. J Enzyme Inhibit Med Chem2013, 28(1):205-213.
[15]Facile spectrophotometric assay of molar equivalents of N-hydroxysuccinimide esters of monomethoxyl poly-(ethylene glycol) derivatives. Chem Cent J 2012; 6(1):142.
[16]Method to screen aromatic ligands in mixtures for quantitative affinities to target using magnetic separation of bound ligands along with HPLC and UV photometry detection. Michrochim Acta2012; 176(1):243-249.
[17]An improved malachite green assay of phosphate: mechanism and application. Anal Biochem 2011; 409(1):144-149.
[18]Homogeneous noncompetitive assay of protein via Förster-resonance-energy-transfer with tryptophan residue(s) as intrinsic donor(s) and fluorescent ligand as acceptor. Biosens Bioelectron 2009; 25(1):112-117.
[19]The measurement of cyclic nucleotide phosphodiesterase 4 activities via the quantification of inorganic phosphate with malachite green. Anal Chim Acta2009; 636(1):105-110.
[20]An integration strategy to estimate the initial rates of enzyme reactions with much expanded linear ranges using uricases as models. Anal Chim Acta 2009; 631(1): 22-28.
[21]廖飞，杨晓兰，胡小蕾，冯一然，种惠敏，李雨薇。一种均相定量比较未纯化酶及其突变体比活性的方法。中国发明专利，ZL 5.X (2018).
[22]廖飞，杨晓兰，胡小蕾，许榜田，王昕，陈春艳，秦家林，谭登，黄亚。双-(对-烷氧基苯丙烯酮)类谷胱甘肽-S-转硫酶潜抑制剂。中国发明专利，ZL 4.4 (2018).
[23]廖飞，龙高波，杨海，冯虎。微纳米材料、其表面用亲水物质共价修饰的产物及制备方法。中国发明专利，ZL4.X (2017).
[24]廖飞，杨晓兰，胡小蕾，秦家林，何琛雄，李元丽，刘霖。一种荧光滴定测定单抗活性位点浓度的方法。中国发明专利，ZL5.6 (2016).
[25]廖飞，杨晓兰，胡小蕾，黄亚，陈远翔，李元丽。一种双试剂法测定尿酸含量试剂盒的配方。中国发明专利，ZL4.1 (2016).
[26]廖飞，杨晓兰，刘红博，党济政，龙高波，李元丽，刘霖。用多波长吸收单通道同步测量多种酶活性的方法。中国发明专利ZL 6.8 (2014).
[27]廖飞，杨晓兰，龙高波，蒲军，刘红博，李元丽。用两端带羧基的直链亲水聚合物包被纳米磁芯制备微纳米磁性材料的方法及其应用。中国发明专利ZL 9.5 (2014).
[28]廖飞，杨晓兰，袁拥华，赵运胜，冯娟，张纯，谢燕玲。作用于血液中低分子物质的大分子药物的体外给药装置及其应用。中国发明专利ZL 0.X (2014).
[29]廖飞，杨晓兰，谢燕玲，邓萍，赵运胜，朱莎，甘志勇，廖娟，刘冬，于明安。联用磁分离与色谱分析测定混合物中配体亲和力的方法。中国发明专利ZL 8.2 (2012).
[30]廖飞，赵运胜，杨晓兰，李想，张纯，冯娟，卜友泉，谢燕玲。一种重组表达苛求芽孢杆菌胞内尿酸酶及其聚乙二醇修饰与应用。中国发明专利 ZL1.1 (2012).

u联系方式
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