中文关键词水稻土镉污染紫色光合细菌16S测序微生物群落分布 英文关键词paddy soilcadmium pollutionpurple photosynthetic bacteria16S sequencingmicrobial community distribution

作者	单位	E-mail
罗路云	长江师范学院现代农业与生物工程学院, 重庆 408100	luoluyun1992@163.com
金德才	中国科学院生态环境研究中心环境生物技术重点实验室, 北京 100085
王殿东	长江师范学院现代农业与生物工程学院, 重庆 408100
陈昂	湖南省农业科学院植物保护研究所园艺作物病虫害治理湖南省重点实验室, 长沙 410125
张德咏	湖南省农业科学院杂交水稻研究中心, 长沙 410125
曾军	新疆农业科学院微生物应用研究所, 乌鲁木齐 830091
匡炜	湖南省农业科学院水稻研究所, 长沙 410125
张卓	湖南省农业科学院植物保护研究所园艺作物病虫害治理湖南省重点实验室, 长沙 410125	lionkingno.1@163.com
刘勇	湖南省农业科学院植物保护研究所园艺作物病虫害治理湖南省重点实验室, 长沙 410125	haoasliu@163.com

中文摘要 稻田重金属污染每年造成水稻产量的巨大损失，重金属污染问题日益受到人们的关注.因此，了解不同镉污染水平下水稻土微生态及理化性质的变化具有重要意义.本研究通过16S测序技术探讨稻田土壤光合细菌群落对不同镉污染水平的响应.结果表明，随着镉污染程度升高，pH值、总镉和有效镉含量逐渐降低.高镉组、中镉组和低镉组的土壤α多样性差异较小，富集的光合细菌种群具有显著差异，光合细菌群落之间也均具有显著差异.高镉组光合细菌物种之间有效连接明显多于中镉和低镉组，连接更紧密且密度更高.碱性氮、pH、有效磷、有效钾、总氮、总磷、有机质、总镉和有效镉是影响光合细菌群落的重要因素，与光合细菌群落显著相关，解释了59.90%的光合细菌群落变异，其中有效镉含量与Methylorubrum populi、Methylorubrum extorquens、Methylobacterium sp.Leaf125和Rhodopseudomonas sp.AAP120显著正相关（R>0.05，P<0.05）.本研究对了解镉污染对稻田土壤特定功能微生物种群的影响具有重要意义. 英文摘要 Heavy metal pollution in rice fields leads to huge losses in rice yield every year and is thus of increasing concern. Therefore, it is important to understand the changes in the microecology and physicochemical properties of paddy soil under different levels of cadmium pollution. The purpose of this study was to investigate the response of the photosynthetic bacterial community in paddy soil to different cadmium pollution levels using 16S sequencing technology. The results showed that pH, total cadmium, and available cadmium content decreased gradually with the increase in cadmium pollution. The soil α diversity was slightly different in the high cadmium (Cd), medium Cd, and low Cd groups; however, the enriched photosynthetic populations and photosynthetic bacterial communities were significantly different among these groups. The effective connections between photosynthetic bacterial species in the high Cd group were significantly greater than those in the medium and low Cd groups, the connections were closer, and the density was higher. Alkaline nitrogen, pH, available (P/K), total (N/P), organic matter, total cadmium, and available cadmium were important factors affecting the photosynthetic bacterial community and were significantly correlated with the photosynthetic bacterial community, explaining 59.90% of the variation in the photosynthetic bacterial community. Effective Cd content was significantly positively correlated with Methylorubrum populi, Methylorubrum extorquens, Methylobacterium sp. Leaf125, and Rhodopseudomonas sp. AAP120 (R>0.05, P<0.05). This study will provide a theoretical basis for the microbial remediation of cadmium contamination in paddy fields. This study is important for understanding the effects of cadmium pollution on specific functional microbial populations in paddy soils.

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