中文关键词两段反硝化碳氮比固体缓释碳源脱氮微生物群落 英文关键词two-stage denitrificationC/Nsolid slow-release carbon sourcedenitrificationmicrobial community

作者	单位	E-mail
唐义	华南理工大学环境与能源学院, 广州 510006	736302457@qq.com
马邕文	华南理工大学环境与能源学院, 广州 510006 广东省植物纤维高值化清洁利用工程技术研究中心, 广州 510641	ppywma@scut.edu.cn
万金泉	华南理工大学环境与能源学院, 广州 510006 广东省植物纤维高值化清洁利用工程技术研究中心, 广州 510641
王艳	华南理工大学环境与能源学院, 广州 510006 广东省植物纤维高值化清洁利用工程技术研究中心, 广州 510641
叶刚	华南理工大学环境与能源学院, 广州 510006

中文摘要 传统生物脱氮工艺处理废水过程中，出水硝态氮（NO₃^--N）浓度过高是导致总氮（TN）超标的主要原因.通过采用外加碳源提高碳氮比（C/N），强化反硝化过程可有效解决这一难题.利用基于两段反硝化工艺原理研发设计的一体化脱氮反应器，通过在第二段反硝化池投加聚丁二酸丁二醇酯（PBS），采用扫描电子显微镜，对反硝化过程前后PBS材料进行表征，采用扩增子测序，深入探究投加PBS前后，第二段反硝化池中微生物群落结构变化.连续120 d的运行数据表明，COD去除率从95.7%降到90.8%，总氮去除率从51.8%提高到80%，Proteobacteria菌门相对丰富度从36.1%上升到46.1%，Thermomonas菌属相对丰富度从6.47%上升到13.48%.结果表明，投加PBS后，PBS不仅可以为反硝化作用提供碳源，其表面还可作为微生物生长附着的载体，起到良好的挂膜作用，同时提高了反硝化菌丰富度，强化了反硝化过程，脱氮性能明显增强，有效提高系统总氮去除率. 英文摘要 During wastewater treatment using a traditional biological denitrification process, the excessive concentration of nitrate nitrogen (NO₃^--N) in the effluent is the primary cause of excessive total nitrogen (TN) generation. By using an external carbon source to increase the carbon to nitrogen ratio (C/N), the denitrification process can be strengthened, which effectively addresses this problem. Using an integrated denitrification reactor developed based on the two-stage denitrification process principle with the addition of polybutylene succinate (PBS) in the second stage, the denitrification process was analyzed using a scanning electron microscope before and after characterization of PBS materials. Moreover, amplicon sequencing was used for in-depth exploration of changes in the microbial community structure in the second denitrification pool before and after the addition of PBS. The data of a continuous 120-day experiment showed that the COD removal rate dropped from 95.7% to 90.8%, the TN removal rate increased from 51.8% to 80%, the relative abundance of Proteobacteria phylum rose from 36.1% to 46.1%, and the relative abundance of Thermomonas rose from 6.47% to 13.48%. The results show that after the addition of PBS, PBS can not only provide carbon source for denitrification, but its surface can also serve as a carrier for microbial growth and attachment, play a good role in filming, and increase the abundance of denitrifying bacteria and strengthen denitrification. During the nitrification process, denitrification performance was significantly enhanced, effectively improving the TN removal rate of the system.

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