中文关键词低温驯化颗粒污泥全自养脱氮反应自由能污泥形态菌群结构 英文关键词cold acclimationgranular sludgecompletely autotrophic nitrogen removalapparent activation energy of reactionsludge morphologybacterial community structure

作者	单位	E-mail
钱飞跃	苏州科技大学环境科学与工程学院, 苏州 215009	qfywater@163.com
刘雨馨	苏州科技大学环境科学与工程学院, 苏州 215009
王建芳	苏州科技大学环境科学与工程学院, 苏州 215009 苏州科技大学天平学院, 苏州 215009 城市生活污水资源化利用技术国家地方联合工程实验室, 苏州 215009	wjf302@163.com
刘文如	苏州科技大学环境科学与工程学院, 苏州 215009 苏州科技大学天平学院, 苏州 215009 江苏高校水处理技术与材料协同创新中心, 苏州 215009

中文摘要 低温驯化是提高部分亚硝化-厌氧氨氧化（PN/A）组合工艺低温脱氮效能和运行稳定性的有效方法.为探究低温驯化对污泥特性的具体影响，本文从温度敏感性、颗粒外观形态、胞外聚合物（EPS）组成和微生物群落结构等方面，对中高温培养（30℃）和低温驯化（15℃）PN/A颗粒污泥之间的差异性进行了分析.反应热力学的研究结果表明，驯化污泥（G_L）在低温区（10~20℃）的脱氮性能较中高温培养污泥（G_H）有了显著提高，总无机氮去除的表观反应活化能（E_a）降低了28.4%.与G_H相比，G_L的平均粒径减小了25.8%，EPS含量增长了16.6%，颗粒的沉降性能明显下降.由高通量测序结果可知，G_L具有更高的菌群多样性，同时，污泥中好氧氨氧化菌（Nitrosomonas）与厌氧氨氧化菌（Candidatus_Kuenenia）的丰度比值（0.04）远小于G_H的0.34.这意味着颗粒污泥在低温环境中对慢速生长自养菌仍具有较强的截留能力.上述发现为解析PN/A污泥在低温条件下的自适应机制，推动组合工艺的工程化应用提供了重要参考. 英文摘要 Cold acclimation is an effective approach for improving the nitrogen removal performance and operational stability of partial nitritation/ANAMMOX (PN/A) combined processes at low temperatures. To explore the specific effects of cold acclimation on the characteristics of sludge, differentiations in temperature sensitivity, granular morphology, composition of extracellular polymer substance (EPS), and bacterial community structure between PN/A granular sludges cultivated at medium-high temperature (30℃) and acclimated to low temperature (15℃) were investigated in this study. The results of reaction thermodynamics showed that the nitrogen removal performance of the granules acclimated to low temperature (G_L) was significantly higher than that of those cultivated at medium-high temperature (G_H) under the low temperature (10-20℃), and the apparent activation energy (E_a) of total inorganic nitrogen removal for the former was decreased by 28.4%. Compared with G_H, G_L had a smaller average particle size of 25.8% and higher EPS contents of 16.6%, resulting in a significant lower settling property. Based on the high-throughput sequencing results, G_L exhibited a higher diversity of bacterial community, and a lower relative abundance ratio (0.04) of aerobic ammonium-oxidizing bacteria (Nitrosomonas) and anaerobic ammonium-oxidizing bacteria (Candidatus_Kuenenia) than 0.34 for G_H. It indicated that the PN/A granules held a strong ability to retain slow-growing autotrophic bacteria in the system, even under low temperatures. These findings could provide meaningful references for analyzing the self-adaption mechanisms of PN/A sludge to low temperature conditions and promote the industrial application of combined processes.

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