中文关键词厌氧氨氧化(ANAMMOX)空间异质性颗粒污泥絮体主流 英文关键词ANAMMOXspatial heterogeneitygranular sludgeflocsmainstream

作者	单位	E-mail
宋家俊	苏州科技大学环境科学与工程学院, 苏州 215009	875078465@qq.com
朱明奕	苏州科技大学环境科学与工程学院, 苏州 215009
郭凯成	苏州科技大学环境科学与工程学院, 苏州 215009
汪倩	苏州科技大学环境科学与工程学院, 苏州 215009
刘文如	苏州科技大学环境科学与工程学院, 苏州 215009 城市生活污水资源化利用技术国家地方联合工程实验室, 苏州 215009 江苏省水处理技术与材料协同创新中心, 苏州 215009	liuwenru1987@126.com
沈耀良	苏州科技大学环境科学与工程学院, 苏州 215009 城市生活污水资源化利用技术国家地方联合工程实验室, 苏州 215009 江苏省水处理技术与材料协同创新中心, 苏州 215009

中文摘要 在控制进水TN浓度<50 mg·L^-1、水力停留时间为2.0 h和水温为20℃条件下，采用连续流完全混合式反应器对比研究了限NH₄⁺和限NO₂^-厌氧氨氧化系统的脱氮效能及微生物种群特征.结果表明，尽管两厌氧氨氧化反应器维持了类似的TN去除负荷［0.45~0.5 kg·（m³·d）^-1］和TN去除率（70%左右），但限NH₄⁺厌氧氨氧化反应器中ΔNO₃^-/ΔNH₄⁺呈现更快的上升趋势.批式试验及高通量测序结果表明，限NH₄⁺厌氧氨氧化反应器比限NO₂^-反应器具有更为显著的功能及微生物种群空间异质性.Candidatus_Brocadia是两反应器中的优势厌氧氨氧化菌属，限NH₄⁺条件下Candidatus_Brocadia在大粒径颗粒污泥中的富集水平（53.9%）显著高于絮体污泥（19.1%）；而在限NO₂^-条件下颗粒污泥与絮体污泥中Candidatus_Brocadia的相对丰度差别不大，分别为28.1%和21.3%.两反应器中均有Nitrospira存在且主要生存于絮体污泥中，对O₂的需求应是驱动Nitrospira于絮体污泥中生长的关键因素；此外，限NH₄⁺（即NO₂^-富余）环境可以促进Nitrospira的生长繁殖.综上，提出了基于选择性排泥的限NH₄⁺厌氧氨氧化系统优化运行策略. 英文摘要 The performance and microbial characteristics of ammonium-limited and nitrite-limited ANAMMOX reactors were studied in two continuously stirred tank reactors. The influent TN concentrations were controlled below 50 mg·L^-1. The hydraulic retention time and water temperature were maintained at 2.0 h and 20℃, respectively. Results showed that though both ANAMMOX reactors demonstrated similar TN removal loading rates[0.45-0.5 kg·(m³·d)^-1] and TN removal efficiencies (around 70%), the ΔNO₃^-/ΔNH₄⁺ ratio of the ammonium-limited ANAMMOX reactor showed a faster upward trend. Batch tests and high-throughput sequencing results indicated that the ammonium-limited ANAMMOX reactor had more significant functional and population heterogeneity than the nitrite-limited ANAMMOX reactor. Candidatus_Brocadia was the predominant ANAMMOX bacteria in both reactors. The relative abundance of Candidatus_Brocadia in large granules (53.9%) was significantly higher than that in flocs (19.1%) under the ammonium-limited conditions, whereas only a small difference in relative abundance of Candidatus_Brocadia was observed between the granules (28.1%) and flocs (21.3%) in the nitrite-limited ANAMMOX reactor. Nitrospira-like NOB were detected in both ANAMMOX reactors, which primarily inhabited flocs, seemingly driven by the availability of oxygen. Moreover, the ammonium-limited (i.e., excess nitrite) conditions seemingly favored the growth of Nitrospira. Building upon these results, a control strategy for optimal operation of the ammonium-limited ANAMMOX reactor was proposed based on selective floc discharge.

PDF全文下载地址:

https://www.hjkx.ac.cn/hjkx/ch/reader/create_pdf.aspx?file_no=20201233&flag=1&journal_id=hjkx&year_id=2020