中文关键词重金属污染重金属固定细菌植物促生细菌高通量测序分子生态网络分析 英文关键词heavy metal contaminationheavy metal-immobilizing bacteriaplant growth-promoting bacteriahigh-throughput sequencingphylogenetic molecular ecological network

作者	单位	E-mail
陈兆进	南阳师范学院农业工程学院, 南水北调中线水源区水安全河南省协同创新中心, 南阳 473061	zhaojin_chen@163.com
李英军	南阳师范学院生命科学与技术学院, 南阳 473061
邵洋	南阳师范学院生命科学与技术学院, 南阳 473061
林立安	南阳师范学院生命科学与技术学院, 南阳 473061
徐鸽	南阳师范学院生命科学与技术学院, 南阳 473061
陈彦	南阳师范学院生命科学与技术学院, 南阳 473061
田伟	生态环境部南京环境科学研究所, 南京 210042
姚伦广	南阳师范学院农业工程学院, 南水北调中线水源区水安全河南省协同创新中心, 南阳 473061
韩辉	南阳师范学院农业工程学院, 南水北调中线水源区水安全河南省协同创新中心, 南阳 473061	17657311626@163.com

中文摘要 重金属污染土壤中具有丰富的微生物群落组成，为生物修复提供了微生物资源.本研究以新乡市某电池厂周边Cd污染土壤为研究对象，采用高通量测序和平板分离方法分析其细菌群落组成.传统培养方法表明新乡市重金属污染土壤细菌由厚壁菌门、放线菌门、变形菌门和拟杆菌门这4个门，芽孢杆菌属、节杆菌属和根瘤菌属等30个属组成；高通量测序表明，其由变形菌门、放线菌门和酸杆菌门等25门及400属组成.相较于培养方法，高通量测序群落组成更为丰富.基于高通量测序的分子生态网路分析表明其关键细菌分别为Arthrobacter、Marmoricola、Nocardioides、Ferruginibacter、Flavitalea、Nitrospira和Lysobacter等组成.对分离的159株可培养菌株进行Cd摇瓶吸附实验，结果表明Aneurinibacillus、Arthrobacter和Bacillus等11个属的30株菌具有较好地固定效果.效果验证实验表明，Ochrobactrum sp.1-6、Bacillus sp.2-11和Pseudomonas sp.1-9等6株高效菌株能提高青菜（鸡毛菜）生物量、降低青菜不同组织中的Cd含量.本研究为新乡市重金属污染土壤修复提供菌种资源，同时为重金属污染土壤细菌群落和功能提供参考依据. 英文摘要 There are many types of bacteria in heavy metal-contaminated soils, including plant growth-promoting bacteria and heavy metal-immobilizing bacteria, which may potentially be used for the bioremediation of contaminated soil. In this study, the Cd-contaminated soil around a battery factory in Xinxiang City was collected and its bacterial community composition was analyzed using high-throughput sequencing (HTS) and the plate lineation separation method. The traditional culture method showed that bacteria isolated from contaminated soil belonged to four phyla (Firmicutes, Actinobacteria, Proteobacteria, and Bacteroidetes) and 30 genera including Bacillus, Arthrobacter, and Rhizobium. HTS showed that the soil contained 25 phyla including Proteobacteria, Actinobacteria, and Acidobacteria and 400 genera. The HTS analysis revealed a greater diversity of the bacterial community than the traditional culture method. Network analysis was performed using the relative abundances of bacteria based on the phylogenetic molecular ecological network (pMEN) method. Network analysis showed that the key bacteria included Arthrobacter, Marmoricola, Nocardioides, Ferruginibacter, Flavitalea, Nitrospira, and Lysobacter. The Cd fixation and adsorption abilities of the 159 cultivable strains were determined. The results showed that 30 strains from 11 genera, including Aneurinibacillus, Arthrobacter, and Bacillus, were highly efficient. Six high-efficiency strains were shown to increase the biomass of pak choi (Brassica chinensis L.) and reduce the content of Cd in different pak choi tissues. This study provides candidate bacterial species for the remediation of heavy metal-contaminated soil in Xinxiang City and provides references for the bacterial community and its function in heavy metal-contaminated soil.

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