中文关键词培养条件厌氧氨氧化颗粒污泥性质微生物群落结构 英文关键词culture conditionsanaerobic ammonium oxidationgranular sludge propertiesmicrobial community structure

作者	单位	E-mail
姜滢	苏州科技大学环境科学与工程学院, 苏州 215009	jying753951@163.com
郭萌蕾	苏州科技大学环境科学与工程学院, 苏州 215009
谢军祥	苏州科技大学环境科学与工程学院, 苏州 215009
常尧枫	苏州科技大学环境科学与工程学院, 苏州 215009
谢嘉玮	苏州科技大学环境科学与工程学院, 苏州 215009
陈重军	苏州科技大学环境科学与工程学院, 苏州 215009 江苏水处理技术与材料协同创新中心, 苏州 215009 江苏省环境科学与工程重点实验室, 苏州 215009	chongjunchen@163.com
沈耀良	苏州科技大学环境科学与工程学院, 苏州 215009 江苏水处理技术与材料协同创新中心, 苏州 215009 江苏省环境科学与工程重点实验室, 苏州 215009

中文摘要 采用膨胀颗粒污泥床（EGSB）和上升式厌氧污泥床（UASB）反应器在不同运行条件下培养厌氧氨氧化颗粒污泥，对比分析颗粒污泥性质和微生物群落的差异性.研究表明接种厌氧氨氧化絮状污泥经过EGSB和UASB反应器运行384 d后，均能实现颗粒化，颗粒污泥平均粒径分别达到1.17 mm和1.21 mm，各范围（<0.2、0.2~1.5、1.5~3和>3 mm）的粒径占比为6.06%、60.05%、25.25%和8.64%，7.40%、58.90%、32.04%和1.66%.扫描电镜结果表明不同运行条件下的污泥菌群均以短杆菌、球型菌为主.高通量测序结果表明，Shannon指数EGSB反应器为7.52高于UASB反应器为7.18；变形菌门（Proteobacteria）是两个反应器各阶段污泥的主要菌门，浮霉菌门（Planctomycetes）从接种时的3.30%增到第384d的12.30%（EGSB）和13.30%（UASB）.EGSB反应器中的主要厌氧氨氧化菌属为Candidatus Brocadia占7.53%，其次为Candidatus Kuenenia 属占1.61%；而在UASB反应器中Candidatus Brocadia属和Candidatus Kuenenia 属分别占比为3.69%和7.54%，Candidatus Kuenenia是其优势厌氧氨氧化菌属.优势菌群丰度与环境因子变化存在联系，Candidatus Brocadia丰度与上升流速（v）、氮容积去除负荷（NRR）呈正相关而与水力停留时间（HRT）呈负相关，Candidatus Kuenenia与氮负荷去除率（NRE）、NRR、HRT呈正相关，而与v呈负相关. 英文摘要 Anaerobic ammonium oxidation (ANAMMOX) granular sludge was cultured during different operating conditions by an expanded granular sludge bed (EGSB) reactor and up-flow anaerobic sludge bed (UASB) reactors, and the characteristics of the granular sludge and microbial community were compared. The results showed that the flocculent ANAMMOX sludge can be granulated after being operated for 384 days by the EGSB and UASB reactors. The average particle size reached 1.17 mm and 1.21 mm, respectively. The particle size ratio of each range (<0.2, 0.2-1.5, 1.5-3, and>3 mm) was 6.06%, 60.05%, 25.25%, and 8.64% in the EGSB reactor, and 7.40%, 58.90%, 32.04%, and 1.66% in the UASB reactor, respectively. The results of scanning electron microscopy showed that the bacterial flora during different operating conditions were mainly Brevibacterium and Cocci aggregates. High-throughput sequencing results showed that the Shannon index of the EGSB reactor was 7.52, higher than the 7.18 of the UASB reactor on day 384; Proteobacteria was the main phylum of the sludge at each stage, and Planctomycetes increased from 3.30% to 12.30% in the EGSB reactor and 13.30% in the UASB reactor on day 384. The main ANAMMOX genera in the EGSB reactor were Candidatus Brocadia, accounting for 7.53%, followed by Candidatus Kuenenia accounting for 1.61%, whereas in the UASB reactor, Candidatus Kuenenia was the dominant anaerobic ammonia genus, accounting for 7.54%, followed by Candidatus Brocadia, which accounted for 3.69%. The proportion of dominant species was related to the change in environmental factors. The proportion of Candidatus Brocadia was positively correlated with the up-flow rate and nitrogen removal rate (NRR), but negatively correlated with hydraulic retention time (HRT). Candidatus Kuenenia was positively correlated with nitrogen removal efficiency (NRE), NRR, and HRT, but negatively correlated with the up-flow rate.

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