中文关键词氮沉降细菌群落多样性高通量测序温带草原 英文关键词nitrogen depositionbacterial communitydiversitypyrosequencing techniquetemperate steppe

作者	单位	E-mail
李宗明	长江大学动物科学学院, 荆州 434025 中国科学院生态环境研究中心城市与区域国家重点实验室, 北京 100085	l_zongming@163.com
沈菊培	中国科学院生态环境研究中心城市与区域国家重点实验室, 北京 100085 中国科学院大学, 北京 100049	jpshen@rcees.ac.cn
张丽梅	中国科学院生态环境研究中心城市与区域国家重点实验室, 北京 100085 中国科学院大学, 北京 100049
刘国平	长江大学动物科学学院, 荆州 434025
白文明	中国科学院植物研究所, 北京 100093
贺纪正	中国科学院生态环境研究中心城市与区域国家重点实验室, 北京 100085 中国科学院大学, 北京 100049

中文摘要 随着社会经济发展，活性氮源随人为活动逐年递增，导致氮沉降相应增加，由此带来的生态环境问题也日益严重.本研究基于细菌16S rRNA基因高通量测序技术，分析长期人工模拟氮沉降氮输入量为0、1、4、8、32 g·（m²·a）^-1对干旱半干旱区草原土壤微生物群落结构的影响.结果表明，模拟氮沉降7 a增加了土壤中可利用氮含量，引起草原土壤酸化.高通量测序结果表明，细菌OTU（operational taxonomy unit）数量随氮输入量的增加呈现增加的趋势，高氮处理[8 g·（m²·a）^-1和32 g·（m²·a）^-1]显著高于低氮和对照处理；细菌α多样性指数呈现先增加后降低的趋势.不同氮输入水平下土壤细菌群落结构差异显著，且与土壤pH和氮的有效性显著相关.不同细菌相对丰度在纲的水平对氮输入的响应趋势不同.氮的长期大量输入可直接或间接通过改变土壤性状而影响草地土壤细菌群落结构和多样性. 英文摘要 With the development of economics, the deposition of available nitrogen in the terrestrial ecosystem is increasing dramatically due to anthropic activities, which negatively impacts the sustainability of the ecosystem ecology. In this study, the effect of long-term stimulated nitrogen deposition[with nitrogen addition of 0, 1, 4, 8, and 32 g·(m²·a)^-1] on the microbial community structure of soil was investigated in a temperate steppe in Inner Mongolia using a pyrosequencing technique targeting the bacterial 16S rRNA gene. The results show that the available nitrogen in soil increases with increasing nitrogen addition, resulting in the decrease of the soil pH. The results of pyrosequencing indicate that soil bacterial OTU (operational taxonomy unit) numbers increase with increasing nitrogen deposition, while bacterial α diversity indices show an initial increase and subsequent decrease. Non-metric multidimensional scaling (NMDS) analysis indicates that the bacterial community structure significantly varies among treatments, which can be largely attributed to the changes in the soil pH and nitrogen content due to nitrogen deposition. At the class level, the relative abundance of different bacterial groups shows a varying trend depending on the nitrogen deposition. This study indicates that long-term nitrogen deposition significantly impacts the bacterial community by changing the soil properties.

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