中文关键词亚硝化厌氧氨氧化微生物包埋溶解氧脱氮效能 英文关键词partial nitrificationanaerobic ammonia oxidation (ANAMMOX)microorganism immobilization technologydissolved oxygen (DO)nitrogen removal efficiency

作者	单位	E-mail
许静怡	同济大学环境科学与工程学院, 上海 200092	1631263@tongji.edu.cn
杜俊	同济大学环境科学与工程学院, 上海 200092
杨一烽	同济大学环境科学与工程学院, 上海 200092
吕锋	同济大学环境科学与工程学院, 上海 200092
夏四清	同济大学环境科学与工程学院, 上海 200092	siqingxia@tongji.edu.cn

中文摘要 分别采用SBR反应器和MBR反应器驯化培养亚硝化污泥和厌氧氨氧化（anaerobic ammonia oxidation，ANAMMOX）污泥，并通过微生物包埋技术将两类污泥分别包埋，构建亚硝化-厌氧氨氧化（partial nitrification-ANAMMOX，PN/A）双菌层系统.短期实验证明该系统中亚硝化菌（ammonia oxidizing bacteria，AOB）和ANAMMOX菌在不同阶段分别起主导作用，维持系统的酸碱平衡，并实现NH₄⁺-N的高效去除（98.8%）.长期实验表明，在溶解氧受限时，PN/A双菌层系统能够有效提高系统对溶解氧的利用效率，并增强系统的稳定性和脱氮效能.在溶解氧为1.0 mg·L^-1，进水NH₄⁺-N质量浓度分别为200 mg·L^-1和400 mg·L^-1时，对照组脱氮效率仅为58.1%和61.4%，而PN/A双菌层系统脱氮效率均稳定在80%左右；溶解氧为3.0 mg·L^-1，进水NH₄⁺-N质量浓度为400 mg·L^-1时，PN/A双菌层系统总氮去除率达87.9%，总氮积累负荷（NLR）为0.4 kg·（m³·d）^-1，总氮去除负荷（NRR）为12.8 mg·（g·h）^-1. 英文摘要 Nitrification and anaerobic ammonia oxidation (ANAMMOX) sludge was acclimated via SBR and MBR reactors respectively, both of which were embedded with microbial immobilization technology and spliced together to construct a PN/A double-bacteria-layer system. A short-term experiment proved that ammonia-oxidizing bacteria (AOB) and ANAMMOX bacteria in the system played dominant roles separately in different stages, which maintained the acid-base balance and achieved efficient removal of NH₄⁺-N (98.8%). A long-term experiment showed that the PN/A double-bacteria-layer system improved the utilization of dissolved oxygen (DO) and enhanced the stability and nitrogen removal efficiency, with limited dissolved oxygen. The nitrogen removal efficiency of the PN/A double-bacteria-layer system remained stable at~80% when the influent NH₄⁺-N was 200 mg·L^-1 or 400 mg·L^-1 in the presence of 1.0 mg·L^-1 DO, whereas those of the control group were only 58.1% and 61.4%, respectively. When influent NH₄⁺-N and DO were 400 mg·L^-1 and 3.0 mg·L^-1, respectively, the PN/A double-bacteria-layer system achieved a nitrogen removal efficiency, nitrogen loading rate (NLR), and nitrogen removal rate (NRR) of 87.9%, 0.4 kg·(m³·d)^-1, and 12.8 mg·(g·h)^-1, respectively.

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