中文关键词黄海微型浮游生物高通量测序技术内转录间隔区(ITS)多样性 英文关键词Yellow Seamicro-planktonhigh-throughput sequencing technologyinternal transcribed space (ITS)diversity

作者	单位	E-mail
张莉	辽宁大学环境学院, 沈阳 110036 中国环境科学研究院, 环境基准与风险评估国家重点实验室, 北京 100012 中国环境科学研究院流域水生态保护技术研究室, 北京 100012	zhangli_03_18@163.com
林佳宁	中国环境科学研究院, 环境基准与风险评估国家重点实验室, 北京 100012 中国环境科学研究院流域水生态保护技术研究室, 北京 100012
张远	中国环境科学研究院, 环境基准与风险评估国家重点实验室, 北京 100012 中国环境科学研究院流域水生态保护技术研究室, 北京 100012	zhangyuan@craes.org.cn
王书平	中国环境科学研究院, 环境基准与风险评估国家重点实验室, 北京 100012 中国环境科学研究院流域水生态保护技术研究室, 北京 100012
张晓娇	中国环境科学研究院, 环境基准与风险评估国家重点实验室, 北京 100012 中国环境科学研究院流域水生态保护技术研究室, 北京 100012 大连海洋大学水产与生命学院, 大连 116023

中文摘要 微型真核浮游生物是海洋生物群落的重要组成部分，对维持海洋生态系统的稳定发挥着重要的作用.本研究对黄海水环境样本的标记基因ITS进行Illumina高通量测序，探讨了黄海微型真核浮游生物的多样性及群落结构特征，并分析了其与环境因子之间的关系.结果表明：①基于标记基因ITS的高通量测序技术可获得较丰富的真菌、浮游植物、浮游动物种类，其中真菌OTUs > 浮游植物OTUs > 浮游动物OTUs，真菌reads数 > 浮游动物reads数 > 浮游植物reads数.②不同水域微型浮游生物的物种丰度及多样性程度不同，Ace指数及Chao1指数表现为南黄海 > 北黄海 > 东海北部 > 冷水团，Simpson指数及Shannon-Wiener指数表现为东海北部 > 北黄海 > 南黄海 > 冷水团，冷水团水域的物种丰度及多样性最低.③随着温度的升高，真菌、浮游植物、浮游动物reads数、OTUs均呈现出上升的趋势；随着盐度的增加，真菌的reads数、OTUs升高，而浮游植物、浮游动物的reads数、OTUs下降，其中浮游植物的reads数、OTUs显著下降（P<0.05）；在北纬30°~40°范围内，随着纬度的升高，真菌、浮游动物的Shannon-Wiener指数上升，而浮游植物Shannon-Wiener指数下降；与海岸线的距离越大，真菌、浮游植物、浮游动物Shannon-Wiener指数越低.本研究首次以ITS为扩增子高通量测序研究海洋微型真核浮游生物，丰富了微型真核浮游生物的认识，揭示了高通量测序技术在该海域研究的可行性，为其他海域微型真核浮游生物的研究提供了借鉴. 英文摘要 Eukaryotic micro-plankton plays a key role in the marine biotic community and in maintaining the stability of the marine ecosystem. In order to determine the diversity and characteristics of eukaryotic micro-plankton in the Yellow Sea, Illumina high-throughput sequencing, based on gene markers of ITS, was conducted. An analysis of the relationship between community structure and environmental factors was conducted as well. The results are summarized in the following. ① Various of species of fungi, phytoplankton, and zooplankton were obtained by high-throughput sequencing technology; for OTUs, the order is fungi > phytoplankton > zooplankton, and for reads, the order is fungi > zooplankton > phytoplankton. ② In different waters, species abundance and diversity differed from each other. The Ace index and Chao1 index demonstrated an ordering of southern Yellow Sea > northern Yellow Sea > northern East Sea > Cold Water Mass; however, for the Simpson index and Shannon-Wiener index, the tendency followed the order of northern East Sea > northern Yellow Sea > southern Yellow Sea > Cold Water Mass. Species abundance and diversity in the Cold Water Mass were lowest. ③ The reads and OTUs of fungi, phytoplankton, and zooplankton increased as temperature rose. The reads and OTUs of fungi increased as salinity increased, while there was an opposite trend for phytoplankton and zooplankton. Latitude was another significant factor. In the range of 30° to 40° north latitude, the Shannon-Wiener index for fungi and zooplankton indicated an increasing tendency with an increase in latitude, while the Shannon-Wiener index for phytoplankton showed an opposite trend. With a greater distance from the coast, the Shannon-Wiener index of fungi, phytoplankton and zooplankton was lower. This is the first study that amplifies the use ITS in the research of marine eukaryotic micro-plankton, giving an enriched understanding of eukaryotic micro-plankton. The feasibility of applying high-throughput sequencing technology in the sea is proven, and this will provide a reference for other researchers.

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