摘要:酚酸物质是影响微生物生物量和群落结构的重要因子之一，研究酚酸物质在不同土地利用方式下的变化规律及其与微生物群落结构的关系，有助于更好地理解不同土地利用方式下微生物群落变化的作用机制。以山河屯林业局奋斗林场次生林（SF）、落叶松人工林（LP）、农田地（FL）和撂荒地（AL）为研究对象，测定不同土地利用方式下0-5 cm、5-10 cm和10-20 cm层的土壤总酚、复合态酚、水溶性酚和9种酚酸物质，并采用磷脂脂肪酸法（PLFA）测定这4种土地利用方式的土壤微生物群落。结果表明，各土层的落叶松人工林土壤总酚含量显著高于其他三种土地利用方式。在0-5 cm和5-10 cm土层中，落叶松人工林土壤水溶性酚含量最高，而在10-20 cm土层中，则是次生林显著高于其余三种土地利用方式（P < 0.05）。在0-5 cm土层中，次生林土壤的总PLFA、真菌含量比农田地和撂荒地分别高14.61%、80.91%和55.63%、156.55%，同时，次生林的土壤真菌：细菌（F：B）显著高于落叶松人工林、农田地和撂荒地（P < 0.05）。0-5 cm层和5-10 cm层的土壤总酚与微生物群落（细菌、真菌）分别呈正相关和负相关关系，而在10-20 cm层，三种土壤酚类物质与微生物群落均未达到显著相关（P > 0.05）。冗余分析表明，0-5 cm层土壤中的阿魏酸、2，4-二羟基苯甲酸和β-谷甾醇均对真菌群落和F：B有显著影响（P < 0.05），而在10-20 cm层中，只有β-谷甾醇影响了微生物群落的生长。土地利用方式的变化改变了表层土壤的酚酸物质含量和微生物群落结构，酚酸物质对表层土壤各类群的微生物量有明显的促进作用，但抑制了深层土壤微生物的生长。



Abstract:Presence of phenolic acids is one of the most important factors that influences the soil microbial community. Investigating changes in phenolic acid composition and its relationship to the soil microbial community structure under different land uses can contribute to a better understanding of mechanisms of microbial community change under different land uses. In this study, we selected natural secondary forest (SF), larch plantation (LP), farm land (FL), and abandoned land (AL) as research sites in the Shanhetun Forestry Bureau. Phospholipid fatty acid (PLFA) approaches were employed to detect the soil microbial community structure among the four land uses. The soil total phenol, complex phenol, water-soluble phenol, and nine phenolic acids in 0-5 cm, 5-10 cm, and 10-20 cm depth soil layers in plots at all land use sites were determined. Our findings were that the total soil phenol content in LP was significantly higher than in soil under other land uses in each of the soil layers tested. Water-soluble phenol content in the 0-5 cm and 5-10 cm soil layers in LP were high for the four land uses, while the water-soluble phenol content was highest in SF in 10-20 cm layer (P < 0.05). In the 0-5 cm soil layer, the total PLFA and fungal content of SF were 14.61%, 80.91%, 55.63% and 156.55% higher than that of FL and AL respectively. Meanwhile, the ratio of fungi to bacteria (F:B) in SF was higher than for other land uses (P < 0.05). Total phenol had a positive and negative relationship with the microbial community (bacteria and fungi) in the 0-5 cm and 5-10 cm layers, respectively, while there was no significant correlation between the three soil phenolic substances and the microbial community in the 10-20 cm layer (P > 0.05). Redundancy analysis showed that ferulic acid, 2,4-dihydroxybenzoic acid, and β-sitosterol in the 0-5 cm layer had significant effects on the fungal community and F:B (P < 0.05), but in 10-20 cm layer soil, only β-sitosterol affected the growth of microbial communities. Changes to land use affected the content of phenolic acids and the microbial community structure in top soil. Phenolic acids significantly promoted microbial communities in the top soil, but inhibited microbial growth at greater soil depths.





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