摘要:为了探究添加生物炭对南方红壤杉木人工林土壤养分含量、酶活性及细菌丰度和群落结构及多样性的影响，分析三者之间的作用机制，基于高通量测序技术，通过添加不同生物炭对人工杉木林土壤环境中细菌群落进行培养和测定，同时挑选与土壤C、N循环相关的4种酶，分析4种酶与土壤养分含量及土壤细菌相对丰度的关系。结果表明：添加生物炭总体使得土壤pH值，全磷、有效磷、速效钾等含量有所提高，同时促进土壤-α-葡萄糖苷酶、土壤-β-葡萄糖苷酶及脲酶的活性，对过氧化氢酶的影响不显著；由于生物炭制备原料不同，其自身性质和物质含量也存在差异，因此杉叶炭对土壤养分的提高量大于木屑炭，而木屑炭处理的土壤全碳含量高于杉叶炭；低添加量生物炭对参与碳循环的土壤葡萄糖苷酶活性提高程度大于高添加量生物炭，而与氮循环有关的土壤脲酶活性随着生物炭添加量的增加而提高，尤其3% BL600处理对土壤脲酶影响十分显著；向土壤添加杉叶炭能够提高土壤细菌丰度，而木屑炭降低了土壤细菌丰度，低温炭大于高温炭；添加生物炭对土壤pH值，全碳、全磷、有效磷、速效钾含量等具有直接影响，土壤组分和性质对不同细菌种群生活习性与功能产生不同的影响，土壤酶活性与土壤细菌存在密切的联系，细菌丰度的变化又会引起土壤酶活性的改变，因此土壤酶活性和细菌群落相对丰度对添加生物炭的响应存在一定的相关关系。



Abstract:This paper aims to explore the interaction mechanism of the soil properties and nutrient contents, enzyme activity of the soil, and the abundance and activity of soil bacterial community structure and diversity, and impact analysis of three in Chinese fir plantation's red soil in southern China after biochars application. We cultivated and determined bacterial community in soil environment of the artificial Chinese fir forest by adding different biochars based on high-throughput sequencing technology. Four enzymes related to soil C and N cycling were selected to analyze the relationship between the four enzymes and the soil nutrient content and relative abundance of the soil bacteria. The results showed that the biochar application improved the soil pH, total phosphorus, available phosphorus, available potassium, and promoted the activities of soil-α-glucosidase, soil-β-glucosidase and urease, but had no significant effect on catalase. The increase of the Chinese fir leaf biochar was greater than fir wood chip biochar, while the total carbon content of soil with fir wood chip biochar was higher than that of the Chinese fir leaf biochar because the raw materials for biochar preparation and its own properties and substance content were different. The activity of C cycle soil-glucosidase increased more with low addition of biochar than with high addition of biochar, while the soil urease activity related to the N cycle increased with the increase of the biochar addition, especially 3% BL600 treatment had a significant effect on soil urease. Adding cedar leaf charcoal to soil could increase the soil bacterial abundance, while wood charcoal reduced the soil bacterial abundance, and low temperature charcoal was higher than high temperature charcoal. Adding biochar had direct influence on the soil pH value, total carbon, total phosphorus, available phosphorus and available potassium content. The soil composition and properties had different effects on the living habits and functions of different bacterial populations. The soil enzyme activities were closely related to soil bacteria. The changes in bacterial abundance caused changes in the soil enzyme activities. Therefore, the soil enzyme activities and bacterial communities relative abundance had a certain correlation with the response to the addition of biochar.





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