周桂娴^1,2,3,
马荣荣⁴,
杨宗英⁵,
胡鲲^1,2,3,,
1. 上海海洋大学, 国家水生动物病原库, 上海 201306;
2. 上海海洋大学, 水产科学国家级实验教学示范中心, 上海 201306;
3. 农业部淡水水产种质资源重点实验室, 上海 201306;
4. 宁波大学, 宁波 315000;
5. 南昌市农业科学院, 南昌 330038

作者简介: 周桂娴(1994-),女,硕士研究生,研究方向为水生动物医学,E-mail:243127601@qq.com.

通讯作者: 胡鲲,khu@shou.edu.cn

基金项目: 国家重点研发计划“蓝色粮仓科技创新”专项“水产病害大数据平台及预警模型构建与应用”课题（2019YFD0900102）；上海市科委项目（18391901500）


中图分类号: X171.5

Acute Toxicity of Prometryn, Phoxim, and Methylene Blue and Their Histopathological Effects on Penaeus vannamei

Zhou Guixian^1,2,3,
Ma Rongrong⁴,
Yang Zongying⁵,
Hu Kun^1,2,3,,
1. National Pathogen Collection Center for Aquatic Animals, Shanghai Ocean University, Shanghai 201306, China;
2. National Demonstration Center for Experimental Fisheries Science Education, Shanghai Ocean University, Shanghai 201306, China;
3. Key Laboratory of Freshwater Aquatic Genetic Resources, Ministry of Agriculture, Shanghai 201306, China;
4. Ningbo University, Ningbo 315000, China;
5. Nanchang Academy of Agricultural Sciences, Nanchang 330038, China

Corresponding author: Hu Kun,khu@shou.edu.cn

CLC number: X171.5

-->

摘要
HTML全文
图(0)表(0)
参考文献(25)
相关文章
施引文献
资源附件(0)
访问统计

摘要:采用静水生物实验法评价扑草净、辛硫磷和亚甲基蓝对凡纳滨对虾（Pemaeus vannamei）的急性毒性风险，为其安全性评估和合理使用提供参考。水温28℃下，3种药物对凡纳滨对虾的24 h半数致死浓度（24 h-LC₅₀）为13.517 mg·L^-1、6.32 μg·L^-1和5.769 mg·L^-1；48 h-LC₅₀分别为5.050 mg·L^-1、1.03 μg·L^-1和4.698 mg·L^-1，安全浓度为0.211 mg·L^-1、0.0083 μg·L^-1和0.935 mg·L^-1。用扑草净、辛硫磷和亚甲基蓝48 h-LC₅₀浓度的1/10进行暴露实验，在0、2、4和6 d取肝胰腺组织和肠道组织做病理切片，得到染毒时间和组织发生病理变化的关系。扑草净暴露4 d和6 d后，凡纳滨对虾肝胰腺出现了少量小管结构破坏，管壁细胞缺失，肠道粘膜层的上皮细胞溶解量增多，胞质呈空泡状。辛硫磷暴露4 d和6 d后，凡纳滨对虾的肝胰腺组织有肝管排列疏松，管壁不完整，细胞缺失，少量管壁细胞脱落入管腔等现象；肠道组织也出现了大面积溶解，局部可见上皮细胞脱落等不良现象。随着亚甲基蓝暴露时间增加，凡纳滨对虾肝胰腺和肠道的组织病变越严重，在暴露4 d和6 d后肝胰腺组织的部分肝管结构破坏，细胞缺失，少量管壁细胞坏死，胞核固缩深染或碎裂溶解；肠道组织在暴露6 d后，黏膜层上皮完全丧失，肠腔内可见少量脱落的上皮细胞。
关键词: 扑草净/
辛硫磷/
亚甲基蓝/
凡纳滨对虾/
急性毒性/
组织病理变化

Abstract:The acute toxicity risk of three chemicals (prometryn, phoxim, and methylene blue) on Penaeus vannamei was evaluated using hydrostatic biological method. At the temperature of 28℃, the 24 h median lethal concentration (24 h-LC₅₀) of prometryn, phoxim, and methylene blue was 13.517 mg·L^-1, 6.32 μg·L^-1 and 5.769 mg·L^-1, respectively. And the 48 h-LC₅₀ was 5.050 mg·L^-1, 1.03 μg·L^-1 and 4.698 mg·L^-1, respectively. Meanwhile, the safe concentrations of prometryn, phoxim, and methylene blue were 0.211 mg·L^-1, 0.0083 μg·L^-1, and 0.935 mg·L^-1, respectively. Exposure experiments were conducted with the chemical concentration of 10% LC₅₀ at 48 h. Hepatopancreas and intestinal tissues were taken for pathological sections at 0, 2, 4 and 6 d, and the relationship between the time of infection and pathological changes in tissues was obtained. After 4 d and 6 d of prometryn exposure, a small number of tubular structures were destroyed in the hepatopancreas of Penaeus vannamei, and the wall cells were missing. At the same time, the dissolved amount of epithelial cells in the intestinal mucosa was increased, and the cytoplasm was vacuole. After exposure to phoxim for 4 d and 6 d, the hepatopancreas of Penaeus vannamei showed loose arrangement of hepatic ducts, incomplete wall, loss of cells, and a small number of wall cells shed into the lumen. Intestinal tissue also showed a large area of dissolution, local epithelial cell shedding and other adverse phenomena. With the increase of exposure time of methylene blue, tissue lesions were more serious in hepatopancreas and intestinal tract of Penaeus vannamei. At 4 d and 6 d after exposure, some hepatic duct structures in hepatopancreas were damaged, cells were lost, a small number of wall cells were necrotic, and nuclei were hyperchromatic or fragmented and dissolved. After 6 d of exposure, the mucosa epithelium was completely lost, and a small number of epithelial cells were shed in the intestinal lumen.
Key words:prometryn/
phoxim/
methylene blue/
Penaeus vannamei/
acute toxicity/
histopathologic change.

刘丽娟, 王玮云, 张华威, 等. 菲律宾蛤仔对扑草净的生物富集与消除规律[J]. 食品科学, 2016, 37(21):252-256Liu L J, Wang W Y, Zhang H W, et al. Accumulation and elimination of prometryn in Ruditapes philippinarum[J]. Food Science, 2016, 37(21):252-256(in Chinese)

Navarro S, Vela N, Giménez M J, et al. Persistence of four s-triazine herbicides in river, sea and groundwater samples exposed to sunlight and darkness under laboratory conditions[J]. The Science of the Total Environment, 2004, 329(1-3):87-97

徐英江, 刘慧慧, 任传博, 等. 莱州湾海域表层海水中三嗪类除草剂的分布特征[J]. 渔业科学进展, 2014, 35(3):34-39Xu Y J, Liu H H, Ren C B, et al. Distributions of the triazine herbicides in the surface seawater of Laizhou Bay[J]. Progress in Fishery Sciences, 2014, 35(3):34-39(in Chinese)

李如茵, 王洪艳, 应锦玲, 等. 气相色谱法测定贝类中扑草净残留方法建立[J]. 农产品加工, 2019(1):49-51 Li R Y, Wang H Y, Ying J L, et al. Study on determination of prometryn residue in shellfish by gas chromatography[J]. Farm Products Processing, 2019(1):49-51(in Chinese)

汪鹏鹏, 张松林, 靳佳, 等. 辛硫磷和敌百虫协同抑制斑马鱼乙酰胆碱酯酶(AChE)活性[J]. 生态毒理学报, 2017, 12(1):298-304Wang P P, Zhang S L, Jin J, et al. Phoxim and trichlorfon synergistically reduce acetylcholinesterase (AChE) activity of zebrafish (Brachydanio rerio)[J]. Asian Journal of Ecotoxicology, 2017, 12(1):298-304(in Chinese)

杨先乐, 湛嘉, 黄艳平. 有机磷农药对水生生物毒性影响的研究进展[J]. 上海水产大学学报, 2002, 11(4):378-382Yang X L, Zhan J, Huang Y P. Progress on research of toxic effect of organophosphorous pesticides on aquatic organism[J]. Journal of Shanghai Fisheries University, 2002, 11(4):378-382(in Chinese)

汤施展, 陈中祥, 白淑艳, 等. 5种水产药物对虹鳟幼鱼的急性毒性[J]. 水产学杂志, 2015, 28(6):33-37Tang S Z, Chen Z X, Bai S Y, et al. Acute toxicity of five drugs in aquaculture on rainbow trout Oncorhynchus mykiss juveniles[J]. Chinese Journal of Fisheries, 2015, 28(6):33-37(in Chinese)

Rico A, van den Brink P J. Probabilistic risk assessment of veterinary medicines applied to four major aquaculture species produced in Asia[J]. The Science of the Total Environment, 2014, 468-469:630-641

Robertson P K J, Black K D, Adams M, et al. A new generation of biocides for control of Crustacea in fish farms[J]. Journal of Photochemistry and Photobiology B:Biology, 2009, 95(1):58-63

Lv Y, Qin Y T, Svec F, et al. Molecularly imprinted plasmonic nanosensor for selective SERS detection of protein biomarkers[J]. Biosensors and Bioelectronics, 2016, 80:433-441

段渝峰, 任鹏宇. 40%乙草胺·扑草净悬乳剂的配方研制[J]. 山东化工, 2015, 44(15):55-57Duan Y F, Ren P Y. The formula development of 40% acetochlor-prometryn suspoemulsion[J]. Shandong Chemical Industry, 2015, 44(15):55-57(in Chinese)

穆瑞珍, 何雷, 王国卿. 30%辛硫磷水乳剂的研究[J]. 山东化工, 2009, 38(9):25-26,30 Mu R Z, He L, Wang G Q. Study on 30%phoxim emulsion in water[J]. Shandong Chemical Industry, 2009, 38(9):25-26,30(in Chinese)

周永欣, 章宗涉, 等. 水生生物毒性试验方法[M]. 北京:农业出版社, 1989:112-114

秦志清, 樊海平, 薛凌展, 等. 常用渔药对异育银鲫"中科3号"幼鱼的急性毒性试验[J]. 科学养鱼, 2016(10):55-56

张彩明, 程鹏君. 4种化学品对脊尾白虾的急性毒性研究[J]. 中国渔业质量与标准, 2014, 4(1):21-25Zhang C M, Cheng P J. Acute toxicity of four kinds of chemicals to Palaemon carincauda[J]. Chinese Fishery Quality and Standards, 2014, 4(1):21-25(in Chinese)

周冬仁, 盛鹏程, 孙博弈, 等. 氰戊菊酯与溴氰菊酯对凡纳对虾的急性毒性[J]. 贵州农业科学, 2018, 46(7):103-105Zhou D R, Sheng P C, Sun B Y, et al. Acute toxicity of fenvalerate and deltamethrin to Penaeus vannamei[J]. Guizhou Agricultural Sciences, 2018, 46(7):103-105(in Chinese)

葛家春, 付龙龙. 重磅权威消息!江苏"杀青苔死蟹事件"涉事产品检测结果出炉:主成分为1种药物,对植物有杀灭作用. 南方农村报,2019-04-19

Stara A, Kristan J, Zuskova E, et al. Effect of chronic exposure to prometryne on oxidative stress and antioxidant response in common carp (Cyprinus carpio L.)[J]. Pesticide Biochemistry and Physiology, 2013, 105(1):18-23

U.S. Environmental Protection Agency (US EPA). Registration Elibility Decision (RED)-Prometryn[R]. Washington DC:US EPA, 1996

Hu X, Li X C, Sun B B, et al. Effects of enrofloxacin on cytochromes P4501A and P4503A in Carassius auratus gibelio (crucian carp)[J]. Journal of Veterinary Pharmacology and Therapeutics, 2012, 35(3):216-223

Wang Y H, Lv L, Yu Y J, et al. Single and joint toxic effects of five selected pesticides on the early life stages of zebrafish (Danio rerio)[J]. Chemosphere, 2017, 170:61-67

Xu Y J, Tian X H, Zhang X Z, et al. Simultaneous determination of malachite green, crystal violet, methylene blue and the metabolite residues in aquatic products by ultra-performance liquid chromatography with electrospray ionization tandem mass spectrometry[J]. Journal of Chromatographic Science, 2012, 50(7):591-597

陈万光, 屈菊平, 周芬娜. 亚甲基蓝对高体鳑鮍鱼苗的急性毒性研究[J]. 广东农业科学, 2010, 37(5):131-132Chen W G, Qu J P, Zhou F N. Study on the acute toxicity of methylene blue to young Rhodeus ocellatus[J]. Guangdong Agricultural Sciences, 2010, 37(5):131-132(in Chinese)

王丹生, 李娟, 王旭. 6种常用渔药对团头鲂幼鱼的急性毒性试验[J]. 辽东学院学报:自然科学版, 2011, 18(2):145-149,153 Wang D S, Li J, Wang X. Acute toxicity test of six aquaculture chemicals on larval of Megalobrama amblycephale[J]. Journal of Eastern Liaoning University:Natural Science, 2011, 18(2):145-149,153(in Chinese)

刘建勇, 彭树锋. 亚甲基蓝对南美白对虾(Pemaeus vannamei Boon)幼体的毒性研究[J]. 海洋湖沼通报, 2005(1):49-53 Liu J Y, Peng S F. Acute toxicity of methylene blue to zoea mysis and post larva of Penaeus vannamei[J]. Transaction of Oceanology and Limnology, 2005(1):49-53(in Chinese)