谢文平,
李海山,
陈会明,
于文莲,
崔媛,
刘伟,
宋乃宁,
沈国林
中国检验检疫科学研究院化学品安全研究所, 北京 100123
作者简介: 周丽丽(1982-),女,助理研究员,研究方向为化学品安全,E-mail:zhoull@aqsiqch.ac.cn.
基金项目: 北京市自然科学基金青年基金(8164071)；质检公益项目(201510203-02，201510024)；院基本科研业务费专项基金(2015JK004，2016JK007，2017JK009，2017JK047)中图分类号: X171.5
Metabolomics Study on the Effect of Triclocarban on the Endogenous Substances in Mice Plasma
Zhou Lili,Xie Wenping,
Li Haishan,
Chen Huiming,
Yu Wenlian,
Cui Yuan,
Liu Wei,
Song Naining,
Shen Guolin
Institute of Chemicals Safety, Chinese Academy of Inspection and Quarantine, Beijing 100123, China
CLC number: X171.5
-->
摘要
HTML全文
图
参考文献
相关文章
施引文献
资源附件
访问统计
摘要:三氯卡班被认定为一种新型的环境污染物，可能对动植物和人体产生危害，为探究三氯卡班的有害作用机制，选择C57BL6小鼠作为载体，利用分子生物学和报告基因方法考察了三氯卡班对肝脏的作用，同时利用代谢组学技术考察三氯卡班对血浆内源性代谢物变化的影响。结果表明，三氯卡班是核受体CAR的激活剂，可以提高肝脏CYP2b10的mRNA表达。同时血浆主要的内源性代谢物也产生了显著性变化，血浆中的脂肪酸比例都呈下降趋势，肉毒碱与乙酰肉碱的比例都呈上升趋势，花生四烯酸与肌酸的比例都呈上升趋势，这都与核受体CAR被激活密切相关，由此说明利用代谢组学技术能够全面地反映三氯卡班所造成的对机体的影响。
关键词: 三氯卡班/
C57BL6小鼠/
激活剂/
代谢组学
Abstract:Triclocarban was recently identified as an environmental pollutant that may cause harm to plants, animals and humans. To explore the mechanism underlying this observation, the effects of triclocarban on the liver were investigated using molecular biology and reporter gene methods with C57BL/6 mice as the carrier. Additionally, we used metabolomics technology to investigate the impact of triclocarban on endogenous metabolites in the plasma. The results demonstrated that triclocarban activated the CAR nuclear receptor and improved hepatic CYP2b10 mRNA expression. Furthermore, endogenous plasma metabolites related to CAR nuclear receptor activation were significantly altered. Specifically, plasma aliphatic acid levels were reduced, while carnitine, acetyl carnitine, arachidonic acid and creatine concentrations were elevated. Thus, we conclude that using metabolomics technology can reveal triclocarban-induced effects on the body.
Key words:triclocarban/
C57BL6 mice/
activator/
metabolomics.
